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Theoretical Description of the Direct Exponential Amplification and Sequencing (DEXAS) Method
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M. Ingr
Veröffentlicht/Copyright:
5. Juli 2005
Abstract
We present a theoretical description of the method of DNA sequencing with simultaneous exponential PCR amplification of the template (DEXAS). Based on the theory of probability, the formula determining the optimal ratio of concentrations of deoxy- and dideoxynucleotides in the reaction mixture is derived, as well as the length distribution of sequenced DNA fragments. The prediction of the number of mutations is given and the theoretically determined aspects of DEXAS are compared with the corresponding quantities of classical sequencing methods. Some other experimentally observed effects are also discussed.
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Published Online: 2005-07-05
Published in Print: 2000-06-21
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
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- Inducibility of the Streptomyces traRts107-Ptra Expression Cassette in Mycobacterium smegmatis
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Artikel in diesem Heft
- Highlight: GTP Binding Proteins Central Regulators in Cell Biology
- Signal Transduction and Post-Transcriptional Gene Expression
- Regulation of GTPases in the Bacterial Translation Machinery
- GTPase Mechanisms and Functions of Translation Factors on the Ribosome
- The Role of Heterotrimeric G Proteins in Platelet Activation
- Upstream and Downstream of Ran GTPase
- Nogo-A, a Potent Inhibitor of Neurite Outgrowth and Regeneration
- Rho GTPases as Targets of Bacterial Protein Toxins
- A Conserved Gβ Binding (GBB) Sequence Motif in Ste20p/PAK Family Protein Kinases
- Identification of a CpG Island in the Human LRP-2 Gene and Analysis of Its Methylation Status in Parathyroid Adenomas
- Theoretical Description of the Direct Exponential Amplification and Sequencing (DEXAS) Method
- Adenine Nucleotide N-Glycosidase Activity of the A-Chain of Cinnamomin Characterized by 1H-Nuclear Magnetic Resonance
- Msb4p, a Protein Involved in Cdc42p-Dependent Organization of the Actin Cytoskeleton, Is a Ypt/Rab-Specific GAP
- Muscle Phosphorylase Kinase Is Not a Substrate of AMP-Activated Protein Kinase
- Cationic Lipopolyamines Induce Degradation of PrPSc in Scrapie-Infected Mouse Neuroblastoma Cells
- Glycosylphosphatidylinositol-Specific Phospholipase D of Human Serum Activity Modulation by Naturally Occurring Amphiphiles
- Retrieval of the mrp2 Gene Encoded Conjugate Export Pump from the Canalicular Membrane Contributes to Cholestasis Induced by tert-Butyl Hydroperoxide and Chloro-Dinitrobenzene
- Matrix Metalloproteinases-2, -3, -7, -9 and -10, But Not MMP-11, Are Differentially Expressed in Normal, Benign Tumorigenic and Malignant Human Keratinocyte Cell Lines
- Human Keratinocyte Cell Lines Differ in the Expression of the Collagenolytic Matrix Metallo-proteinases-1, -8, and -13 and of TIMP-1
- Inducibility of the Streptomyces traRts107-Ptra Expression Cassette in Mycobacterium smegmatis
- Shortest Known Prion Protein Allele in Highly BSE-Susceptible Lemurs
- Prostaglandin Deficiency Promotes Sensitization of Adenylyl Cyclase
