Adenine Nucleotide N-Glycosidase Activity of the A-Chain of Cinnamomin Characterized by 1H-Nuclear Magnetic Resonance
-
Abstract
Plant ribosome-inactivating proteins specifically cleave an N-glycosidic bond of a unique adenosine in the largest ribosomal RNA, releasing an adenine from ribosomes of different sources. Here, 1H-nuclear magnetic resonance is used to analyze the enzymatic products of the A-chain of cinnamomin, a type-II ribosome-inactivating protein (RIP) acting on the nucleotides in situ. The enzymatic activities of the RIP on nine nucleotides are compared. Cinnamomin A-chain can cleave the N-glycosidic bond and release an adenine base from adenine nucleotides except 5′-ATP; however, it cannot act on 5′-GMP, 5′-CMP, and 5′-UMP. The A-chain in the mixture of cinnamomin A- and B-chain exhibits higher activity toward adenine nucleotides than the A-chain alone does, suggesting that the B-chain can conformationally stabilize the A-chain. Intact cinnamomin also exhibits lower activity toward adenine nucleotides. However, cinnamomin B-chain and heat-denatured intact cinnamomin cannot hydrolyze all the tested nucleotides. We conclude that hydrolysis of the N-C glycosidic bond of nucleotide compounds by cinnamomin A-chain has a base preference, and the negatively charged phosphate group(s) reduces the recognition ability of the A-chain to adenine nucleotide.
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Highlight: GTP Binding Proteins Central Regulators in Cell Biology
- Signal Transduction and Post-Transcriptional Gene Expression
- Regulation of GTPases in the Bacterial Translation Machinery
- GTPase Mechanisms and Functions of Translation Factors on the Ribosome
- The Role of Heterotrimeric G Proteins in Platelet Activation
- Upstream and Downstream of Ran GTPase
- Nogo-A, a Potent Inhibitor of Neurite Outgrowth and Regeneration
- Rho GTPases as Targets of Bacterial Protein Toxins
- A Conserved Gβ Binding (GBB) Sequence Motif in Ste20p/PAK Family Protein Kinases
- Identification of a CpG Island in the Human LRP-2 Gene and Analysis of Its Methylation Status in Parathyroid Adenomas
- Theoretical Description of the Direct Exponential Amplification and Sequencing (DEXAS) Method
- Adenine Nucleotide N-Glycosidase Activity of the A-Chain of Cinnamomin Characterized by 1H-Nuclear Magnetic Resonance
- Msb4p, a Protein Involved in Cdc42p-Dependent Organization of the Actin Cytoskeleton, Is a Ypt/Rab-Specific GAP
- Muscle Phosphorylase Kinase Is Not a Substrate of AMP-Activated Protein Kinase
- Cationic Lipopolyamines Induce Degradation of PrPSc in Scrapie-Infected Mouse Neuroblastoma Cells
- Glycosylphosphatidylinositol-Specific Phospholipase D of Human Serum Activity Modulation by Naturally Occurring Amphiphiles
- Retrieval of the mrp2 Gene Encoded Conjugate Export Pump from the Canalicular Membrane Contributes to Cholestasis Induced by tert-Butyl Hydroperoxide and Chloro-Dinitrobenzene
- Matrix Metalloproteinases-2, -3, -7, -9 and -10, But Not MMP-11, Are Differentially Expressed in Normal, Benign Tumorigenic and Malignant Human Keratinocyte Cell Lines
- Human Keratinocyte Cell Lines Differ in the Expression of the Collagenolytic Matrix Metallo-proteinases-1, -8, and -13 and of TIMP-1
- Inducibility of the Streptomyces traRts107-Ptra Expression Cassette in Mycobacterium smegmatis
- Shortest Known Prion Protein Allele in Highly BSE-Susceptible Lemurs
- Prostaglandin Deficiency Promotes Sensitization of Adenylyl Cyclase
Articles in the same Issue
- Highlight: GTP Binding Proteins Central Regulators in Cell Biology
- Signal Transduction and Post-Transcriptional Gene Expression
- Regulation of GTPases in the Bacterial Translation Machinery
- GTPase Mechanisms and Functions of Translation Factors on the Ribosome
- The Role of Heterotrimeric G Proteins in Platelet Activation
- Upstream and Downstream of Ran GTPase
- Nogo-A, a Potent Inhibitor of Neurite Outgrowth and Regeneration
- Rho GTPases as Targets of Bacterial Protein Toxins
- A Conserved Gβ Binding (GBB) Sequence Motif in Ste20p/PAK Family Protein Kinases
- Identification of a CpG Island in the Human LRP-2 Gene and Analysis of Its Methylation Status in Parathyroid Adenomas
- Theoretical Description of the Direct Exponential Amplification and Sequencing (DEXAS) Method
- Adenine Nucleotide N-Glycosidase Activity of the A-Chain of Cinnamomin Characterized by 1H-Nuclear Magnetic Resonance
- Msb4p, a Protein Involved in Cdc42p-Dependent Organization of the Actin Cytoskeleton, Is a Ypt/Rab-Specific GAP
- Muscle Phosphorylase Kinase Is Not a Substrate of AMP-Activated Protein Kinase
- Cationic Lipopolyamines Induce Degradation of PrPSc in Scrapie-Infected Mouse Neuroblastoma Cells
- Glycosylphosphatidylinositol-Specific Phospholipase D of Human Serum Activity Modulation by Naturally Occurring Amphiphiles
- Retrieval of the mrp2 Gene Encoded Conjugate Export Pump from the Canalicular Membrane Contributes to Cholestasis Induced by tert-Butyl Hydroperoxide and Chloro-Dinitrobenzene
- Matrix Metalloproteinases-2, -3, -7, -9 and -10, But Not MMP-11, Are Differentially Expressed in Normal, Benign Tumorigenic and Malignant Human Keratinocyte Cell Lines
- Human Keratinocyte Cell Lines Differ in the Expression of the Collagenolytic Matrix Metallo-proteinases-1, -8, and -13 and of TIMP-1
- Inducibility of the Streptomyces traRts107-Ptra Expression Cassette in Mycobacterium smegmatis
- Shortest Known Prion Protein Allele in Highly BSE-Susceptible Lemurs
- Prostaglandin Deficiency Promotes Sensitization of Adenylyl Cyclase
