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A Role of Poly (ADP-Ribose) Polymerase in NF- B Transcriptional Activation
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P. O. Hassa
Published/Copyright:
June 1, 2005
Abstract
The transcription factor NF-κB plays a critical role in immune and inflammatory responses. Here we show that poly (ADP ribose) polymerase (PARP) is required for specific NF-κB transcriptional activation in vivo. The activation of the HIV-LTR promoter and an NF-κBdependent artificial promoter was drastically reduced in PARP (_/_) cells, independently of the signaling pathway through which NF-bB was induced. Furthermore NF-κB-dependent gene activation was restored in vivo by the expression of PARP in PARP (_/_) cells. Finally, we show that both NF-κB and PARP formed a stable immunoprecipitable nuclear complex. This interaction did not need DNA binding. Our results suggest that PARP is an important cofactor in the activation cascade of NF-κB-dependent target genes.
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Published Online: 2005-06-01
Published in Print: 1999-07-01
Copyright © 1999 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
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- Cyclin Dependent Kinases and Regulation of the Fission Yeast Cell Cycle
- Paper of the Year 1998
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- Prospects for the Precise Engineering of Plant Genomes by Homologous Recombination
- The Glycosphingolipidoses from Disease to Basic Principles of Metabolism
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- Genetically Engineered and Synthetic Allergen Derivatives: Candidates for Vaccination against Type I Allergy
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- Chimeric Restriction Enzymes: What Is Next?
- Viroids with Hammerhead Ribozymes: Some Unique Structural and Functional Aspects with Respect to Other Members of the Group
- Mutagenesis via Insertional or Restriction Enzyme-Mediated Integration (REMI) as a Tool to Tag Pathogenicity Related Genes in Plant Pathogenic Fungi
- Role of Mitochondria in Parkinson Disease
- Mitochondria Harbouring Mutant mtDNA a Cuckoo in the Nest?
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- The Role of Chemokines in Cutaneous Allergic Inflammation
- Mutations of Calcium Channel beta Subunit Genes in Mice
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