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The Epstein-Barr Virus Nuclear Antigen 2 (EBNA2), a Protein Required for B Lymphocyte Immortalization, Induces the Synthesis of Type I Interferon in Burkitts Lymphoma Cell Lines
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K. Kanda
Veröffentlicht/Copyright:
1. Juni 2005
Abstract
Epstein-Barr virus nuclear antigen 2 (EBNA2), a protein involved in cell transformation, interferes with the cellular response to type I interferons (IFN-α/β). We investigated the function of conditionally expressed EBNA2 in the context of the IFN response in Burkitt's lymphoma cell lines. Expression of EBNA2 led to the transcriptional activation of both endogenous or transfected IFN-stimulated genes (ISGs), genes which contain within their promoters either the interferon-stimulated response element (ISRE) or the gamma interferon activation site (GAS). In search of a molecular mechanism for the transcriptional induction of ISGs, we observed an EBNA2-dependent synthesis of IFN-β mRNA at low levels and the secretion of low amounts of IFN. A transfected IFN-β promoter responded to EBNA2 activation, and a sequence closely resembling a RBP-Jκ binding site was pinpointed as a potential target of EBNA2 activity. EBNA2-dependent transcriptional induction of the IFN-β promoter occurred in EBV-negative Burkitt's lymphoma cells, indicating that other EBV genes were not required for the induction of IFN-β synthesis.
Published Online: 2005-6-1
Published in Print: 1999-2-1
Copyright © 1999 by Walter de Gruyter GmbH & Co. KG
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Artikel in diesem Heft
- Editors Note
- Shoulder Hopping on Giants
- Moving from Zurich to Vienna Up or Down?
- The IMP and European Molecular Biology: Moving the Centre Eastwards
- Max, the Vienna Biocenter and Other Spin Off
- The Functions of Breast Cancer Susceptibility Gene 1 (BRCA1) Product and Its Associated Proteins
- Lymphocyte Antigen Receptor Signal Integration and Regulation by the SHC Adaptor
- New Insights into Signal Recognition and Elongation Arrest Activities of the Signal Recognition Particle
- Being at the Right Place at the Right Time: The Role of Nuclear Transport in Dynamic Transcriptional Regulation in Yeast
- Plectin: A Cytolinker by Design
- Regulation of the Sea Urchin Early H2A Histone Gene Expression Depends on the Modulator Element and on Sequences Located near the 3′ End
- A Ribosomal Orphon Sequence from <I>Xenopus laevis</I> Flanked by Novel Low Copy Number Repetitive Elements
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- A Novel Way to Induce Erythroid Progenitor Self Renewal: Cooperation of c-Kit with the Erythropoietin Receptor
- An Unexpected Role for p53 in Augmenting SV40 Large T Antigen-Mediated Tumorigenesis
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- Regulation of Human c-Abl Tyrosine Kinase Activity in <I>Xenopus</I> Oocytes and Acceleration of Progesterone-Induced G2/M Transition by Oncogenic Forms
- Cmdr1, a Chicken P-Glycoprotein, Confers Multidrug Resistance and Interacts with Estradiol
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