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Transient Transfection of Mammary Epithelial Cells with a PEI/DNA/Adenovirus System

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Published/Copyright: June 1, 2005
Biological Chemistry
From the journal Biological Chemistry Volume 380 Issue 2

Abstract

Studies on epithelial cells often require the transient expression of exogenous proteins in polarized epithelial cells. However, the major limitation of this approach has been the difficulty of obtaining transient gene expression in polarized epithelial cell cultures. We report here on the application of a polyethylenimine (PEI)/DNA/adenovirus system for efficient transient gene expression in mammary epithelial cells. Based on luciferase assay and FACScan analysis the PEI/DNA/adenovirus system is shown to be an effective and simple method for transfecting epithelial cells.

Published Online: 2005-6-1
Published in Print: 1999-2-1

Copyright © 1999 by Walter de Gruyter GmbH & Co. KG

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  1. Editors Note
  2. Shoulder Hopping on Giants
  3. Moving from Zurich to Vienna Up or Down?
  4. The IMP and European Molecular Biology: Moving the Centre Eastwards
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  6. The Functions of Breast Cancer Susceptibility Gene 1 (BRCA1) Product and Its Associated Proteins
  7. Lymphocyte Antigen Receptor Signal Integration and Regulation by the SHC Adaptor
  8. New Insights into Signal Recognition and Elongation Arrest Activities of the Signal Recognition Particle
  9. Being at the Right Place at the Right Time: The Role of Nuclear Transport in Dynamic Transcriptional Regulation in Yeast
  10. Plectin: A Cytolinker by Design
  11. Regulation of the Sea Urchin Early H2A Histone Gene Expression Depends on the Modulator Element and on Sequences Located near the 3′ End
  12. A Ribosomal Orphon Sequence from <I>Xenopus laevis</I> Flanked by Novel Low Copy Number Repetitive Elements
  13. Characterization of the Transcription Factor MTF-1 from the Japanese Pufferfish (<I>Fugu rubripes</I>) Reveals Evolutionary Conservation of Heavy Metal Stress Response
  14. A Novel Way to Induce Erythroid Progenitor Self Renewal: Cooperation of c-Kit with the Erythropoietin Receptor
  15. An Unexpected Role for p53 in Augmenting SV40 Large T Antigen-Mediated Tumorigenesis
  16. The Epstein-Barr Virus Nuclear Antigen 2 (EBNA2), a Protein Required for B Lymphocyte Immortalization, Induces the Synthesis of Type I Interferon in Burkitts Lymphoma Cell Lines
  17. Regulation of Human c-Abl Tyrosine Kinase Activity in <I>Xenopus</I> Oocytes and Acceleration of Progesterone-Induced G2/M Transition by Oncogenic Forms
  18. Cmdr1, a Chicken P-Glycoprotein, Confers Multidrug Resistance and Interacts with Estradiol
  19. Probing Ribosome Structure by Europium-Induced RNA Cleavage
  20. The Oct-1 POU Domain Directs Developmentally Regulated Nuclear Translocation in Xenopus Embryos
  21. Constitutive Expression of Interleukin-1beta (IL-1beta) in Rat Oligodendrocytes
  22. Conformational Study of the HIV-1 Reverse Transcriptase Inhibitor 1-[(2-Hydroxyethoxy)methyl]-6-(phenylthio)thymine (HEPT)
  23. Transient Transfection of Mammary Epithelial Cells with a PEI/DNA/Adenovirus System
https://doi.org/10.1515/BC.1999.036

Articles in the same Issue

  1. Editors Note
  2. Shoulder Hopping on Giants
  3. Moving from Zurich to Vienna Up or Down?
  4. The IMP and European Molecular Biology: Moving the Centre Eastwards
  5. Max, the Vienna Biocenter and Other Spin Off
  6. The Functions of Breast Cancer Susceptibility Gene 1 (BRCA1) Product and Its Associated Proteins
  7. Lymphocyte Antigen Receptor Signal Integration and Regulation by the SHC Adaptor
  8. New Insights into Signal Recognition and Elongation Arrest Activities of the Signal Recognition Particle
  9. Being at the Right Place at the Right Time: The Role of Nuclear Transport in Dynamic Transcriptional Regulation in Yeast
  10. Plectin: A Cytolinker by Design
  11. Regulation of the Sea Urchin Early H2A Histone Gene Expression Depends on the Modulator Element and on Sequences Located near the 3′ End
  12. A Ribosomal Orphon Sequence from <I>Xenopus laevis</I> Flanked by Novel Low Copy Number Repetitive Elements
  13. Characterization of the Transcription Factor MTF-1 from the Japanese Pufferfish (<I>Fugu rubripes</I>) Reveals Evolutionary Conservation of Heavy Metal Stress Response
  14. A Novel Way to Induce Erythroid Progenitor Self Renewal: Cooperation of c-Kit with the Erythropoietin Receptor
  15. An Unexpected Role for p53 in Augmenting SV40 Large T Antigen-Mediated Tumorigenesis
  16. The Epstein-Barr Virus Nuclear Antigen 2 (EBNA2), a Protein Required for B Lymphocyte Immortalization, Induces the Synthesis of Type I Interferon in Burkitts Lymphoma Cell Lines
  17. Regulation of Human c-Abl Tyrosine Kinase Activity in <I>Xenopus</I> Oocytes and Acceleration of Progesterone-Induced G2/M Transition by Oncogenic Forms
  18. Cmdr1, a Chicken P-Glycoprotein, Confers Multidrug Resistance and Interacts with Estradiol
  19. Probing Ribosome Structure by Europium-Induced RNA Cleavage
  20. The Oct-1 POU Domain Directs Developmentally Regulated Nuclear Translocation in Xenopus Embryos
  21. Constitutive Expression of Interleukin-1beta (IL-1beta) in Rat Oligodendrocytes
  22. Conformational Study of the HIV-1 Reverse Transcriptase Inhibitor 1-[(2-Hydroxyethoxy)methyl]-6-(phenylthio)thymine (HEPT)
  23. Transient Transfection of Mammary Epithelial Cells with a PEI/DNA/Adenovirus System
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