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Regulation of Human c-Abl Tyrosine Kinase Activity in <I>Xenopus</I> Oocytes and Acceleration of Progesterone-Induced G2/M Transition by Oncogenic Forms

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Published/Copyright: June 1, 2005
Biological Chemistry
From the journal Biological Chemistry Volume 380 Issue 2

Abstract

Deregulated activity of the Abl protein tyrosine kinase is oncogenic in humans and in animals. The normal cellular form of the enzyme is maintained at a low state of activity by mechanisms that have not yet been entirely elucidated. In particular, little is known about the trans-acting cellular factors involved. We have tested the activity of human c-Abl microinjected into oocytes of Xenopus laevis. In contrast to versions of Abl capable of transforming mammalian cells, which were highly active when introduced into oocytes, the activity of wild type c-Abl was inhibited. Oncogenic forms of Abl efficiently enhanced the ability of Xenopus oocytes to enter M phase following stimulation by progesterone. Abl-enhanced maturation was normal as judged by accumulation of Mos as well as activation of MAP kinase and Cdc2/CyclinB (MPF). Concomitant with maturation and activation of these kinases, Abl became extensively phosphorylated. Altogether, this suggests that an SH3 domain-dependent Abl regulation mechanism similar to the one observed in mammalian cells operates in Xenopus oocytes. Maturation enhancement by microinjection into Xenopus oocytes represents a useful novel assay for analyzing Abl activity. Moreover, the Xenopus oocyte may be a convenient source of trans-acting Abl regulators for biochemical studies.

Published Online: 2005-6-1
Published in Print: 1999-2-1

Copyright © 1999 by Walter de Gruyter GmbH & Co. KG

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  1. Editors Note
  2. Shoulder Hopping on Giants
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  4. The IMP and European Molecular Biology: Moving the Centre Eastwards
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  6. The Functions of Breast Cancer Susceptibility Gene 1 (BRCA1) Product and Its Associated Proteins
  7. Lymphocyte Antigen Receptor Signal Integration and Regulation by the SHC Adaptor
  8. New Insights into Signal Recognition and Elongation Arrest Activities of the Signal Recognition Particle
  9. Being at the Right Place at the Right Time: The Role of Nuclear Transport in Dynamic Transcriptional Regulation in Yeast
  10. Plectin: A Cytolinker by Design
  11. Regulation of the Sea Urchin Early H2A Histone Gene Expression Depends on the Modulator Element and on Sequences Located near the 3′ End
  12. A Ribosomal Orphon Sequence from <I>Xenopus laevis</I> Flanked by Novel Low Copy Number Repetitive Elements
  13. Characterization of the Transcription Factor MTF-1 from the Japanese Pufferfish (<I>Fugu rubripes</I>) Reveals Evolutionary Conservation of Heavy Metal Stress Response
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  15. An Unexpected Role for p53 in Augmenting SV40 Large T Antigen-Mediated Tumorigenesis
  16. The Epstein-Barr Virus Nuclear Antigen 2 (EBNA2), a Protein Required for B Lymphocyte Immortalization, Induces the Synthesis of Type I Interferon in Burkitts Lymphoma Cell Lines
  17. Regulation of Human c-Abl Tyrosine Kinase Activity in <I>Xenopus</I> Oocytes and Acceleration of Progesterone-Induced G2/M Transition by Oncogenic Forms
  18. Cmdr1, a Chicken P-Glycoprotein, Confers Multidrug Resistance and Interacts with Estradiol
  19. Probing Ribosome Structure by Europium-Induced RNA Cleavage
  20. The Oct-1 POU Domain Directs Developmentally Regulated Nuclear Translocation in Xenopus Embryos
  21. Constitutive Expression of Interleukin-1beta (IL-1beta) in Rat Oligodendrocytes
  22. Conformational Study of the HIV-1 Reverse Transcriptase Inhibitor 1-[(2-Hydroxyethoxy)methyl]-6-(phenylthio)thymine (HEPT)
  23. Transient Transfection of Mammary Epithelial Cells with a PEI/DNA/Adenovirus System
https://doi.org/10.1515/BC.1999.030

Articles in the same Issue

  1. Editors Note
  2. Shoulder Hopping on Giants
  3. Moving from Zurich to Vienna Up or Down?
  4. The IMP and European Molecular Biology: Moving the Centre Eastwards
  5. Max, the Vienna Biocenter and Other Spin Off
  6. The Functions of Breast Cancer Susceptibility Gene 1 (BRCA1) Product and Its Associated Proteins
  7. Lymphocyte Antigen Receptor Signal Integration and Regulation by the SHC Adaptor
  8. New Insights into Signal Recognition and Elongation Arrest Activities of the Signal Recognition Particle
  9. Being at the Right Place at the Right Time: The Role of Nuclear Transport in Dynamic Transcriptional Regulation in Yeast
  10. Plectin: A Cytolinker by Design
  11. Regulation of the Sea Urchin Early H2A Histone Gene Expression Depends on the Modulator Element and on Sequences Located near the 3′ End
  12. A Ribosomal Orphon Sequence from <I>Xenopus laevis</I> Flanked by Novel Low Copy Number Repetitive Elements
  13. Characterization of the Transcription Factor MTF-1 from the Japanese Pufferfish (<I>Fugu rubripes</I>) Reveals Evolutionary Conservation of Heavy Metal Stress Response
  14. A Novel Way to Induce Erythroid Progenitor Self Renewal: Cooperation of c-Kit with the Erythropoietin Receptor
  15. An Unexpected Role for p53 in Augmenting SV40 Large T Antigen-Mediated Tumorigenesis
  16. The Epstein-Barr Virus Nuclear Antigen 2 (EBNA2), a Protein Required for B Lymphocyte Immortalization, Induces the Synthesis of Type I Interferon in Burkitts Lymphoma Cell Lines
  17. Regulation of Human c-Abl Tyrosine Kinase Activity in <I>Xenopus</I> Oocytes and Acceleration of Progesterone-Induced G2/M Transition by Oncogenic Forms
  18. Cmdr1, a Chicken P-Glycoprotein, Confers Multidrug Resistance and Interacts with Estradiol
  19. Probing Ribosome Structure by Europium-Induced RNA Cleavage
  20. The Oct-1 POU Domain Directs Developmentally Regulated Nuclear Translocation in Xenopus Embryos
  21. Constitutive Expression of Interleukin-1beta (IL-1beta) in Rat Oligodendrocytes
  22. Conformational Study of the HIV-1 Reverse Transcriptase Inhibitor 1-[(2-Hydroxyethoxy)methyl]-6-(phenylthio)thymine (HEPT)
  23. Transient Transfection of Mammary Epithelial Cells with a PEI/DNA/Adenovirus System
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