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Antioxidant and binding properties of methanol extracts from indigo plant leaves

  • Yun-Jum Park EMAIL logo , Chang-Sik Shin , Bo-Eun Kim , Gil-Yong Cheon , Jong-Hyang Bae , Yang-Gyu Ku , Su-Min Park , Buk-Gu Heo , Dae-Guk Kim , Ja-Yong Cho and Shela Gorinstein
Published/Copyright: June 24, 2014
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Chemical Papers
From the journal Chemical Papers Volume 68 Issue 10

Abstract

This study was conducted to clarify the effect of ultra-pressure treatment on the extraction of bioactive compounds from indigo plant leaves (Polygonum tinctorium Lour.) and on their properties. Leaves were harvested the in mid-August, 2013, from Naju City (Korea), and treated using two methods: ultra-pressure (550 MPa, 3 min) and hot-air (70°C, 24 h). Then, the leaves were ultrasonically extracted with methanol. The content of indirubin in leaves treated with ultra pressure and hot air was (535.55 ± 26.14) mg kg−1 and (52.63 ± 6.45) mg kg−1, respectively, and that of tryptanthrin was (165.55 ± 8.74) mg kg−1 and (153.00 ± 7.62) mg kg−1, respectively. Polyphenolic content in the leaves extract was (127.24 ± 13.67) mg kg−1 after the ultrapressure and (88.22 ± 5.33) mg kg−1 after the hot-air treatment. The content of flavonoids was (2298.67 ± 83.27) mg kg−1 after the ultra-pressure and (3224.00 ± 21.45) mg kg−1 after the hotair treatment. Di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) radical scavenging activities of the indigo extract obtained by ultra-pressure and hot-air treatment methods at the concentration of 1 mg mL−1 were (80.25 ± 0.73) % and (66.54 ± 2.35) %, respectively, and 2,2′-azinobis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging activities were estimated as (90.14 ± 0.79) % and (64.45 ± 8.97) %, respectively. The methanol leaf extract after ultra-pressure treatment exhibited higher binding properties to human serum albumin in comparison with catechin and conventional treatments. Consequently, it was assumed that the ultra-pressure treatment is an effective method for the extraction of bioactive compounds from indigo leaves.

Keywords: indigo leaves; bioactive compounds; antioxidant activity; binding properties

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Published Online: 2014-6-24
Published in Print: 2014-10-1

© 2014 Institute of Chemistry, Slovak Academy of Sciences

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https://doi.org/10.2478/s11696-014-0572-4
Keywords for this article
indigo leaves; bioactive compounds; antioxidant activity; binding properties

Articles in the same Issue

  1. Chemical preparation and applications of silver dendrites
  2. Evaluation of antioxidant activity and DNA cleavage protection effect of naphthyl hydroxamic acid derivatives through conventional and fluorescence microscopic methods
  3. Modelling of ORL1 receptor-ligand interactions
  4. Kinetics of enantioselective liquid-liquid extraction of phenylglycine enantiomers using a BINAP-copper complex as chiral selector
  5. Diffusive transport of Cu(II) ions through thin ion imprinted polymeric membranes
  6. Magnetic mixed matrix membranes in air separation
  7. The use of impregnated perlite as a heterogeneous catalyst for biodiesel production from marula oil
  8. Nitrobenzene degradation by micro-sized iron and electron efficiency evaluation
  9. RP-HPLC-UV-ESI-MS phytochemical analysis of fruits of Conocarpus erectus L.
  10. Residue analysis of fosthiazate in cucumber and soil by QuEChERS and GC-MS
  11. Degradation of polylactide using basic ionic liquid imidazolium acetates
  12. Ring-opening polymerisation of ɛ-caprolactone catalysed by Brønsted acids
  13. Click synthesis by Diels-Alder reaction and characterisation of hydroxypropyl methylcellulose-based hydrogels
  14. Preparation and physical properties of chitosan-coated calcium sulphate whiskers
  15. A facile synthetic route for antineoplastic drug GDC-0449
  16. Two new frameworks for biphenyl-3,3′,5,5′-tetracarboxylic acid and nitrogen-containing organics
  17. Antioxidant and binding properties of methanol extracts from indigo plant leaves
  18. Dithiols as more effective than monothiols in protecting biomacromolecules from free-radical-mediated damage: in vitro oxidative degradation of high-molar-mass hyaluronan
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