Identification and determination of relatedness of lactobacilli using different DNA amplification methods
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Kristýna Turková
,
Bohuslav Rittich
Abstract
Several DNA amplification-based methods were used for identification and evaluation of the relation between lactobacilli isolated from breastfed full-term infant faeces (31 strains), dairy products (5 strains) and silage (1 strain). Twenty-seven strains isolated from infant faeces were identified as Lactobacillus rhamnosus (9), Lactobacillus gasseri (6), Lactobacillus paracasei (4), Lactobacillus fermentum (4), Lactobacillus salivarius (2), Lactobacillus plantarum (1), and Lactobacillus helveticus (1) using 10 species-specific polymerase chain reactions (PCRs), multiplex PCR for the Lactobacillus casei group, and sequencing of 16S rDNA. Four strains were not identified. Six strains isolated from dairy products and silage were identified as Lactobacillus rhamnosus. A repetitive extragenic palindromic polymerase chain reaction (rep-PCR) with primer (GTG)5 and a randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) with primer M13 were used for confirmation of species identification. Fingerprints were used for evaluation of the relatedness of lactobacilli. Differences between strains from infant faeces, dairy products, and silage were not detected.
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© 2012 Institute of Chemistry, Slovak Academy of Sciences
Articles in the same Issue
- 5th Meeting on Chemistry & Life 2011
- Induction of Cryptococcus laurentii α-galactosidase
- Incorporation of β-(1,6)-linked glucooligosaccharides (pustulooligosaccharides) into plant cell wall structures
- Metal-metabolomics of microalga Chlorella sorokiniana growing in selenium- and iodine-enriched media
- Metabolomic approach to Alzheimer’s disease diagnosis based on mass spectrometry
- Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration
- Identification and determination of relatedness of lactobacilli using different DNA amplification methods
- Production of Geotrichum candidum polygalacturonases via solid state fermentation on grape pomace
- Monitoring of yeast population isolated during spontaneous fermentation of Moravian wine
- Biodegradable polyhydroxybutyrate as a polyol for elastomeric polyurethanes
- Conformational changes in humic acids in aqueous solutions
- Preparation and properties of cementitious composites for geothermal applications
Articles in the same Issue
- 5th Meeting on Chemistry & Life 2011
- Induction of Cryptococcus laurentii α-galactosidase
- Incorporation of β-(1,6)-linked glucooligosaccharides (pustulooligosaccharides) into plant cell wall structures
- Metal-metabolomics of microalga Chlorella sorokiniana growing in selenium- and iodine-enriched media
- Metabolomic approach to Alzheimer’s disease diagnosis based on mass spectrometry
- Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration
- Identification and determination of relatedness of lactobacilli using different DNA amplification methods
- Production of Geotrichum candidum polygalacturonases via solid state fermentation on grape pomace
- Monitoring of yeast population isolated during spontaneous fermentation of Moravian wine
- Biodegradable polyhydroxybutyrate as a polyol for elastomeric polyurethanes
- Conformational changes in humic acids in aqueous solutions
- Preparation and properties of cementitious composites for geothermal applications
