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Monitoring of yeast population isolated during spontaneous fermentation of Moravian wine

  • Hana Šuranská EMAIL logo , Dana Vránová , Jiřina Omelková and Renáta Vadkertiová
Published/Copyright: June 22, 2012
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Chemical Papers
From the journal Chemical Papers Volume 66 Issue 9

Abstract

In enology, yeasts play an important role in the characteristics of the final product. They are predominant in the biochemical interaction with components of must. Rapid identification of the yeast population is necessary for fermentation process monitoring and for obtaining a good quality wine. The main goal of this study was the isolation and characterisation of the yeast microbial community naturally present on grape berries, leaves and occurring during the spontaneous fermentation process of the white wine Veltlin green from the South Moravian region, Czech Republic. The results, based on PCR-RFLP of the 5.8S-ITS region of rDNA, PCR-fingerprinting using microsatellite oligonucleotide primers (GAG)5, (GTG)5, (GAC)5, and M13 primer, showed great diversity of the yeast population. Including grape berries and fermented must, the following yeast species were identified: Hanseniaspora uvarum, Aureobasidium pullulans, Metschnikowia pulcherrima, Torulaspora delbrueckii, a number of Pichia species such as P. fermentans, P. membranifaciens, P. kluyveri, also Sporidiobolus salmonicolor, Rhodosporidium toruloides, Rhodotorula mucilaginosa, Rhodotorula glutinis as well as Saccharomyces cerevisiae and Saccharomyces bayanus. Monitoring of the yeast strains during the wine fermentation process of traditional Moravian wine can contribute to the improvement of wine quality.

Keywords: wine yeasts; identification; PCR-RFLP; PCR-fingerprinting

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Published Online: 2012-6-22
Published in Print: 2012-9-1

© 2012 Institute of Chemistry, Slovak Academy of Sciences

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https://doi.org/10.2478/s11696-012-0198-3
Keywords for this article
wine yeasts; identification; PCR-RFLP; PCR-fingerprinting

Articles in the same Issue

  1. 5th Meeting on Chemistry & Life 2011
  2. Induction of Cryptococcus laurentii α-galactosidase
  3. Incorporation of β-(1,6)-linked glucooligosaccharides (pustulooligosaccharides) into plant cell wall structures
  4. Metal-metabolomics of microalga Chlorella sorokiniana growing in selenium- and iodine-enriched media
  5. Metabolomic approach to Alzheimer’s disease diagnosis based on mass spectrometry
  6. Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration
  7. Identification and determination of relatedness of lactobacilli using different DNA amplification methods
  8. Production of Geotrichum candidum polygalacturonases via solid state fermentation on grape pomace
  9. Monitoring of yeast population isolated during spontaneous fermentation of Moravian wine
  10. Biodegradable polyhydroxybutyrate as a polyol for elastomeric polyurethanes
  11. Conformational changes in humic acids in aqueous solutions
  12. Preparation and properties of cementitious composites for geothermal applications
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