Electrochemical quantification of biomarker myeloperoxidase

Javier Hoyo; Arnau Bassegoda; Tzanko Tzanov

doi:10.1515/znc-2021-0274

Article

Electrochemical quantification of biomarker myeloperoxidase

Javier Hoyo , Arnau Bassegoda and Tzanko Tzanov

Published/Copyright: February 22, 2022

Published by

Become an author with De Gruyter Brill

Submit Manuscript Author Information

From the journal Zeitschrift für Naturforschung C Volume 77 Issue 7-8

Abstract

Point of care testing (PoCT) devices permit precise and rapid detection of disease-related biomarkers contributing to an early disease diagnosis and administration of an appropriate treatment. The enzyme myeloperoxidase (MPO) is a relevant biomarker for infection and inflammation events assessment; however its direct electrochemical quantification is hindered by the limited accessibility to the iron atom in its active center. Herein, such hindrance of the MPO biomolecule is overcome using the redox mediator 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). The charge involved in the electrochemical reduction of the MPO-oxidized ABTS is correlated with the concentration of MPO. The use of ABTS allowed for the electrochemical assessment of a wide range of MPO concentrations (10–1000 nM) including those reported for wound infections, chronic obstructive pulmonary disease and early adverse cardiac events. The developed electroanalytical approach is rapid and inexpensive, and thus suitable for implementation in PoCT devices.

Keywords: ABTS; disease biomarker; electrochemical detection; myeloperoxidase

Corresponding author: Tzanko Tzanov, Department of Chemical Engineering, Grup de Biotecnologia Molecular i Industrial, Universitat Politècnica de Catalunya, Rambla Sant Nebridi 22, 08222, Terrasa, Spain, E-mail: tzanko.tzanov@upc.edu

Funding source: Ministerio de Ciencia e Innovacion

Award Identifier / Grant number: PID2019-104111RB-I00

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: This work was supported by the Spanish Ministerio de Ciencia e Innovacion, (Project PID2019-104111RB-I00).
Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

References

1. Ivanova, K, Ramon, E, Hoyo, J, Tzanov, T. Innovative approaches for controlling clinically relevant biofilms: current trends and future prospects. Curr Top Med Chem 2017;17:1889–914. https://doi.org/10.2174/1568026617666170105143315.Search in Google Scholar

2. Hampton, MB, Kettle, AJ, Winterbourn, CC. Inside the neutrophil phagosome: oxidants, myeloperoxidase, and bacterial killing. J Am Soc Hematol 1998;92:3007–17. https://doi.org/10.1182/blood.v92.9.3007.421k47_3007_3017.Search in Google Scholar

3. Bassegoda, A, Ferreres, G, Pérez-Rafael, S, Hinojosa-Caballero, D, Torrent-Burgués, J, Tzanov, T. New myeloperoxidase detection system based on enzyme-catalysed oxidative synthesis of a dye for paper-based diagnostic devices. Talanta 2019;194:469–74. https://doi.org/10.1016/j.talanta.2018.10.065.Search in Google Scholar PubMed

4. Stefanov, I, Hinojosa-Caballero, D, Maspoch, S, Hoyo, J, Tzanov, T. Enzymatic synthesis of a thiolated chitosan-based wound dressing crosslinked with chicoric acid. J Mater Chem B 2018;6:7943–53. https://doi.org/10.1039/c8tb02483a.Search in Google Scholar PubMed

5. Francesko, A, Ivanova, K, Hoyo, J, Pérez-Rafael, S, Petkova, P, Fernandes, MM, et al.. Bottom-up layer-by-layer assembling of antibacterial freestanding nanobiocomposite films. Biomacromolecules 2018;19:3628–36. https://doi.org/10.1021/acs.biomac.8b00626.Search in Google Scholar PubMed

6. Zhu, A, Ge, D, Zhang, J, Teng, Y, Yuan, C, Huang, M, et al.. Sputum myeloperoxidase in chronic obstructive pulmonary disease. Eur J Med Res 2014;19:1–11. https://doi.org/10.1186/2047-783X-19-12.Search in Google Scholar PubMed PubMed Central

7. Brennan, M-L, Penn, MS, Ven Lente, F, Nambi, V, Shishehbor, MH, Aviles, RJ, et al.. Prognostic value of myeloperoxidase in patients with chest pain. N Engl J Med 2003;349:1695–702. https://doi.org/10.1056/NEJMoa035003.Search in Google Scholar PubMed

8. Ferrante, G, Nakano, M, Prati, F, Niccoli, G, Mallus, MT, Ramazzotti, V, et al.. High levels of systemic myeloperoxidase are associated with coronary plaque erosion in patients with acute coronary syndromes: a clinicopathological study. Circulation 2010;122:2505–13. https://doi.org/10.1161/circulationaha.110.955302.Search in Google Scholar

9. Vestbo, J, Hurd, SS, Agustí, AG, Jones, PW, Vogelmeier, C, Anzueto, A, et al.. Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease GOLD executive summary. Am J Respir Crit Care Med 2013;187:347–65. https://doi.org/10.1164/rccm.201204-0596pp.Search in Google Scholar PubMed

10. Nie, Z, Deiss, F, Liu, X, Akbulut, O, Whitesides, GM. Integration of paper-based microfluidic devices with commercial electrochemical readers. Lab Chip 2010;10:3163–9. https://doi.org/10.1039/c0lc00237b.Search in Google Scholar PubMed PubMed Central

11. Hoyo, J, Guaus, E, Torrent-Burgués, J. Monogalactosyldiacylglycerol and digalactosyldiacylglycerol role, physical states, applications and biomimetic monolayer films. Eur Phys J E 2016;39:1–11. https://doi.org/10.1140/epje/i2016-16039-0.Search in Google Scholar PubMed

12. Zhao, L, Li, X, Lin, Y, Yang, L, Yu, P, Mao, L. Electrochemical impedance spectroscopic measurements of FCCP-induced change in membrane permeability of MDCK cells. Analyst 2012;137:2199–204. https://doi.org/10.1039/c2an35064e.Search in Google Scholar PubMed

13. Hoyo, J, Torrent-Burgués, J, Tzanov, T. Physical states and thermodynamic properties of model Gram-negative bacterial inner membranes. Chem Phys Lipids 2019;218:57–64. https://doi.org/10.1016/j.chemphyslip.2018.12.003.Search in Google Scholar PubMed

14. Martín-Yerga, D. Electrochemical detection and characterization of nanoparticles with printed devices. Biosensors 2019;9:1–23. https://doi.org/10.3390/bios9020047.Search in Google Scholar PubMed PubMed Central

15. Uberman, PM, Pérez, LA, Martín, SE, Lacconi, GI. Electrochemical synthesis of palladium nanoparticles in PVP solutions and their catalytic activity in Suzuki and Heck reactions in aqueous medium. RSC Adv 2014;4:12330–41. https://doi.org/10.1039/c3ra47854h.Search in Google Scholar

16. Zou, C, Yang, B, Bin, D, Wang, J, Li, S, Yang, P, et al.. Electrochemical synthesis of gold nanoparticles decorated flower-like graphene for high sensitivity detection of nitrite. J Colloid Interface Sci 2017;488:135–41. https://doi.org/10.1016/j.jcis.2016.10.088.Search in Google Scholar PubMed

17. Hoyo, J, Guaus, E, Torrent-Burgués, J, Sanz, F. Biomimetic monolayer films of digalactosyldiacylglycerol incorporating plastoquinone. Biochim Biophys Acta - Biomembr 2015;1848:1341–51. https://doi.org/10.1016/j.bbamem.2015.03.003.Search in Google Scholar PubMed

18. Hoyo, J, Guaus, E, Torrent-Burgués, J, Sanz, F. Electrochemical behaviour of mixed LB films of ubiquinone - DPPC. J Electroanal Chem 2012;669:6–13. https://doi.org/10.1016/j.jelechem.2012.01.020.Search in Google Scholar

19. Attar, AM, Richardson, MB, Speciale, G, Majumdar, S, Dyer, RP, Sanders, EC, et al.. Electrochemical quantification of glycated and non-glycated human serum albumin in synthetic urine. ACS Appl Mater Interfaces 2019;11:4757–65. https://doi.org/10.1021/acsami.8b16071.Search in Google Scholar PubMed PubMed Central

20. Giménez-Gómez, P, Gutiérrez-Capitán, M, Capdevila, F, Puig-Pujol, A, Fernández-Sánchez, C, Jiménez-Jorquera, C. Monitoring of malolactic fermentation in wine using an electrochemical bienzymatic biosensor for l-lactate with long term stability. Anal Chim Acta 2016;905:126–33. https://doi.org/10.1016/j.aca.2015.11.032.Search in Google Scholar PubMed

21. Braunschmid, V, Fuerst, S, Perz, V, Zitzenbacher, S, Hoyo, J, Fernandez-sanchez, C, et al.. A fungal ascorbate oxidase with unexpected laccase activity. Int J Mol Sci 2020;21:1–14. https://doi.org/10.3390/ijms21165754.Search in Google Scholar

22. Li, Y, Zhang, J, Huang, X, Wang, T. Construction and direct electrochemistry of orientation controlled laccase electrode. Biochem Biophys Res Commun 2014;446:201–5. https://doi.org/10.1016/j.bbrc.2014.02.084.Search in Google Scholar

23. Bandodkar, AJ, Jia, W, Yardimci, C, Wang, X, Ramirez, J, Wang, J. Tattoo-based noninvasive glucose monitoring: a proof-of-concept study. Anal Chem 2015;87:394–8. https://doi.org/10.1021/ac504300n.Search in Google Scholar

24. Zhang, D, Lu, Y, Zhang, Q, Liu, L, Li, S, Yao, Y, et al.. Protein detecting with smartphone-controlled electrochemical impedance spectroscopy for point-of-care applications. Sensors Actuators, B Chem [Internet 2016;222:994–1002. https://doi.org/10.1016/j.snb.2015.09.041.Search in Google Scholar

25. Guo, J. Uric acid monitoring with a smartphone as the electrochemical analyzer. Anal Chem 2016;88:11986–9. https://doi.org/10.1021/acs.analchem.6b04345.Search in Google Scholar

26. Moral-Vico, J, Barallat, J, Abad, L, Olivé-Monllau, R, Muñoz-Pascual, FX, Galán Ortega, A, et al.. Dual chronoamperometric detection of enzymatic biomarkers using magnetic beads and a low-cost flow cell. Biosens Bioelectron 2015;69:328–36. https://doi.org/10.1016/j.bios.2015.02.042.Search in Google Scholar

27. Lin, KC, Kunduru, V, Bothara, M, Rege, K, Prasad, S, Ramakrishna, BL. Biogenic nanoporous silica-based sensor for enhanced electrochemical detection of cardiovascular biomarkers proteins. Biosens Bioelectron 2010;25:2336–42. https://doi.org/10.1016/j.bios.2010.03.032.Search in Google Scholar

28. Davies, MJ. Myeloperoxidase-derived oxidation: mechanisms of biological damage and its prevention. J Clin Biochem Nutr 2011;48:8–9. https://doi.org/10.3164/jcbn.11-006FR.Search in Google Scholar

29. Won, K, Kim, YH, An, ES, Lee, YS, Song, BK. Horseradish peroxidase-catalyzed polymerization of cardanol in the presence of redox mediators. Biomacromolecules 2004;5:1–4. https://doi.org/10.1021/bm034325u.Search in Google Scholar

30. Bourbonnais, R, Paice, MG. Oxidation of non-phenolic substrates. FEBS Lett 1990;267:99–102. https://doi.org/10.1016/0014-5793(90)80298-w.Search in Google Scholar

31. Hasmann, A, Wehrschuetz-Sigl, E, Marold, A, Wiesbauer, H, Schoeftner, R, Gewessler, U, et al.. Analysis of myeloperoxidase activity in wound fluids as a marker of infection. Ann Clin Biochem 2013;50:245–54. https://doi.org/10.1258/acb.2011.010249.Search in Google Scholar PubMed

32. More, SS, Renuka, PS, Pruthvi, K, Swetha, M, Malini, S, Veena, SM. Isolation, purification, and characterization of fungal laccase from Pleurotus sp. Enzym Res 2011;2011:1–8. https://doi.org/10.4061/2011/248735.Search in Google Scholar PubMed PubMed Central

33. Bathoorn, E, Liesker, JJW, Postma, DS, Koëter, GH, van der Toorn, M, van der Heide, S, et al.. Change in inflammation in out-patient COPD patients from stable phase to a subsequent exacerbation. Int J COPD 2009;4:101–9. https://doi.org/10.2147/copd.s4854.Search in Google Scholar PubMed PubMed Central

34. Zhang, X, Wang, Z, Xie, H, Sun, R, Cao, T, Paudyal, N, et al.. Development of a magnetic nanoparticles-based screen-printed electrodes (MNPs-SPEs) biosensor for the quantification of ochratoxin A in cereal and feed samples. Toxins (Basel) 2018;10:1–12. https://doi.org/10.3390/toxins10080317.Search in Google Scholar PubMed PubMed Central

35. Noiphung, J, Songjaroen, T, Dungchai, W, Henry, CS, Chailapakul, O, Laiwattanapaisal, W. Electrochemical detection of glucose from whole blood using paper-based microfluidic devices. Anal Chim Acta 2013;788:39–45. https://doi.org/10.1016/j.aca.2013.06.021.Search in Google Scholar PubMed

Received: 2021-10-31

Accepted: 2022-02-04

Published Online: 2022-02-22

Published in Print: 2022-07-26

You are currently not able to access this content.

Articles in the same Issue

https://doi.org/10.1515/znc-2021-0274

Keywords for this article

ABTS; disease biomarker; electrochemical detection; myeloperoxidase