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Cytotoxicity test and antibacterial assay on the compound produced by the isolation and modification of artonin E from Artocarpus kemando Miq.

  • Tati Suhartati EMAIL logo , Vicka Andini , Ilham Ramadhan , Yandri Yandri and Sutopo Hadi
Published/Copyright: April 11, 2022
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Physical Sciences Reviews
From the journal Physical Sciences Reviews Volume 8 Issue 10

Abstract

Artocarpus kemando Miq. is an Indonesian endemic plant rich in flavonoid compounds and bioactivity. Artonin E (compound 1), a flavonoid compound, was isolated from the branch and root bark of the indicated plant, obtained from Karang Anyar, Klaten, Penengahan, South Lampung. Compound (1) was then modified using diazomethane, which produced compound (2). Both compounds were then identified by TLC using standard compound, after which their UV–Vis, FT-IR, 1H and 13C NMR spectra were compared with literature data. Furthermore, the cytotoxicity assay of the compound (1) was determined using leukemia P-388 cancer cells, which showed a strong anticancer activity with IC50 of 1.56 μg/mL. The antibacterial activity of this compound against Bacillus subtilis showed strong activity for 0.3 mg/disc concentration; compound (2) with 0.5 mg/disc concentration, while against Escherichia coli, compound (1) showed moderate activity, while compound (2) did not show any activity.

Keywords: A. kemando Miq.; artonin E; B. subtilis ; diazomethane; E. coli ; leukemia P-388 cancer cells
Corresponding author: Tati Suhartati, Department of Chemistry, Universitas Lampung, Bandar Lampung, 35145, Indonesia, E-mail:

Funding source: Direktorat Jenderal Pendidikan Tinggi

Award Identifier / Grant number: 120/E4.1/AK.04.PT/2021

Award Identifier / Grant number: 3972/UN26.21/PN/2021

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: The author appreciates the Ministry of Education, Culture, Research and Technology, for the financial support in form of Basic Research 2021 with contract numbers of 120/E4.1/AK.04.PT/2021, 12 July 2021 and 3972/UN26.21/PN/2021, 14 July 2021.

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

References

1. Mabberley, DJ. The plant-book, 2nd ed. Cambridge: Cambridge University Press; 1997. p. 58.Search in Google Scholar

2. Chen, CY, Cheng, MJ, Kuo, SH, Kuo, SY, Lo, WL. Secondary metabolites from stems of Artocarpus heterophyllus. Chem Nat Compd 2010;46:638–40. https://doi.org/10.1007/s10600-010-9698-y.Search in Google Scholar

3. Jagtap, UB, Bapat, VA. Artocarpus: a review of its traditional uses, phytochemistry and pharmacology. J Ethnopharmacol 2010;129:142–66. https://doi.org/10.1016/j.jep.2010.03.031.Search in Google Scholar PubMed

4. Baliga, MS, Shivashankara, AR, Haniadka, R, Dsouza, J, Bhat, HP. Phytochemistry, nutritional and pharmacological properties of Artocarpus heterophyllus Lam (Jackfruit): a review. Food Res Int 2011;44:1800–11. https://doi.org/10.1016/j.foodres.2011.02.035.Search in Google Scholar

5. Boonlaksiri, C, Oonanant, W, Kongsaeree, P, Kittakoop, P, Tanticharoen, M, Thebtaranonth, Y. An antimalarial stilbene from Artocarpus integer. Phytochemistry 2000;54:415–7. https://doi.org/10.1016/s0031-9422(00)00074-1.Search in Google Scholar PubMed

6. Suhartati, T, Wulandari, GS, Yandri, HS. The antibacterial and anticancer test of cyclomulberochromen compounds from Artocarpus altilis. Res J Chem Environ 2019;23:1–16.Search in Google Scholar

7. Lu, YH, Lin, CN, Ko, HH, Yang, SZ, Tsao, LT, Wang, JP. Two novel and anti-inflammatory constituens of Artocarpus rigida. Helv Chim Acta 2002;85:1626–32. https://doi.org/10.1002/1522-2675(200206)85:6<1626::aid-hlca1626>3.0.co;2-0.10.1002/1522-2675(200206)85:6<1626::AID-HLCA1626>3.0.CO;2-0Search in Google Scholar

8. Weng, JR, Chan, SC, Lu, YH, Lin, HC, Ko, HH, Lin, CN. Antiplatelet prenylflavonoids from Artocarpus communis. Phytochemistry 2006;67:824–9. https://doi.org/10.1016/j.phytochem.2006.01.030.Search in Google Scholar

9. Teo, SH, Ee, GCL, Lim, CK, Rahmani, M, Bong, CFJ. Chemical constituents of Artocarpus kemando (Moraceae). Asian J Chem 2010;23:74–6.Search in Google Scholar

10. Suhartati, T, Achmad, SA, Aimi, N, Hakim, EH, Kitajima, M, Takayama, H, et al.. Artoindonesianin L, a new prenylated flavone with cytotoxic activity from Artocarpus rotunda. Fitoterapia 2001;72:912–8. https://doi.org/10.1016/s0367-326x(01)00343-4.Search in Google Scholar

11. Seo, EK, Lee, D, Shin, YG, Chai, HB, Navarro, HA, Kardono, LBS, et al.. Bioactive prenylated flavonoids from the stem bark of Artocarpus kemando. Arch Pharm Res (Seoul) 2003;26:124–7. https://doi.org/10.1007/BF02976656.Search in Google Scholar

12. Ee, GCL, Teo, SH, Rahmani, M, Lim, CK, Lim, YM, Go, R. Artomandin, a new xanthone from Artocarpus kemando (Moraceae). Nat Prod Res 2014;25:995–1003. 2014. https://doi.org/10.1080/14786419.2010.534471.Search in Google Scholar

13. Arndt, F. Nitrosomethyl urea and diazomethane synthesis. Org Synth 1935;15:48.10.15227/orgsyn.015.0048Search in Google Scholar

14. Marby, TJ, Markham, KR, Thomas, MB. The systematic identification of flavonoids. Berlin: Springer-Verlag; 1970.10.1007/978-3-642-88458-0Search in Google Scholar

15. Suhartati, T, Hernawan, Suwandi, JF, Yandri, HS. Isolation of artonin E from the root bark of Artocarpus rigida, Synthesis of artonin E Acetate and evaluation of anticancer activity. Maced J Chem Chem Eng 2018;37:35–42. https://doi.org/10.20450/mjcce.2018.1406.Search in Google Scholar

16. Alley, MC, Scudiero, DA, Monks, A, Hursey, ML, Czerwinski, MJ, Fine, DL, et al.. Feasibility of drug screening with panels of man tumor cell lines using a microculture tetrazolium assay. Cancer Res 1988;48:589–601.Search in Google Scholar

17. Ito, C, Itoigawa, M, Takakura, T, Ruang Rungsi, N, Enjo, F, Tokuda, H, et al.. Chemical constituens of Garcinia fusca: structure elucidation of eight new xanthones and their cancer chemopreventive activity. J Nat Prod 2003;66:200–5. https://doi.org/10.1021/np020290s.Search in Google Scholar PubMed

18. Bauer, AW, Kirby, WM, Sherris, JC, Turck, M. Antibiotic susceptibility testing by a standarized single disk method. Am J Clin Pathol 1966;45:493–6. https://doi.org/10.1093/ajcp/45.4_ts.493.Search in Google Scholar

19. Davis, WW, Stout, TR. Disc plate method of microbiological antibiotic assay. Appl Mcrobiol 1971;22:659–65. https://doi.org/10.1128/am.22.4.659-665.1971.Search in Google Scholar PubMed PubMed Central

20. Alcaraz, LE, Blanco, SE, Puig, ON, Tomas, F, Ferretti, FH. Antibacterial activity of flavonoids against methicillin-resistant Staphylococcus aureus strains. J Theor Biol 2000;205:231–40. https://doi.org/10.1006/jtbi.2000.2062.Search in Google Scholar PubMed

21. Miller, SI. Antibiotic resistance and regulation of the gram-negative bacterial outer membrane barrier by host innate immune molecules. mBio 2016;7:e01541-16. https://doi.org/10.1128/mBio.01541-16.Search in Google Scholar PubMed PubMed Central

22. Breitmair, E. Structure elucidation by NMR in organic chemistry: practical guide. Chichester: John Wiley and Son, LTD.; 2002.10.1002/0470853069Search in Google Scholar

23. Suhartati, T, Fatimah, N, Yandri, Y, Kurniawan, R, Bahri, S, Hadi, S. The anticancer, antimalarial, and antibacterial activities of moracalkon a isolated from Artocarpus kemando Miq. J Adv Pharm Educ Res 2021;11:105–10. https://doi.org/10.51847/9nhxpcqzud.Search in Google Scholar

24. Hadi, S, Rilyanti, M. Synthesis and in vitro anticancer activity of some organotin(IV) benzoate compounds. Orient J Chem 2010;26:775–9.Search in Google Scholar

25. Hadi, S, Rilyanti, M, Suharso. In vitro activity and comparative studies of some organotin(IV) benzoate derivatives against leukemia cancer cell: L-1210. Indo J Chem 2012;12:172–7. https://doi.org/10.22146/ijc.21359.Search in Google Scholar

26. Hadi, S, Lestari, S, Suhartati, S, Qudus, HI, Rilyanti, M, Herasari, D, et al.. Synthesis and comparative study on the antibacterial activity organotin (IV) 3-hydroxybenzoate compounds. Pure Appl Chem 2021;93:623–8. https://doi.org/10.1515/pac-2020-1103.Search in Google Scholar

27. Samsuar, S, Simanjuntak, W, Qudus, HI, Yandri, Y, Herasari, H, Hadi, S. In vitro antimicrobial activity study of some organotin(IV) chlorobenzoates against Staphylococcus aureus and Escherichia coli. J Adv Pharm Educ Res 2021;11:17–22. https://doi.org/10.51847/kaijzkafco.Search in Google Scholar

28. Hadi, S, Samsuar, S, Qudus, HI, Simanjuntak, W. In vitro antibacterial activity of some of dibutyltin (IV) chlorobenzoate derivatives against Staphylococcus aureus and Escherichia coli. ARPN J Eng Appl Sci 2021;16:1623–9.Search in Google Scholar
Published Online: 2022-04-11

© 2022 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/psr-2021-0140
Keywords for this article
A. kemando Miq.; artonin E; B. subtilis; diazomethane; E. coli; leukemia P-388 cancer cells

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. Recent endeavors in microbial remediation of micro- and nanoplastics
  4. Metal nanoparticles and its application on phenolic and heavy metal pollutants
  5. The story of nitrogen
  6. Recent development of imidazole derivatives as potential anticancer agents
  7. Indole based prostate cancer agents
  8. Lawsone (2-hydroxy-1,4-naphthaquinone) derived anticancer agents
  9. Small modular nuclear reactors are mostly bad policy
  10. A holistic environmental investigation of complementary energy in Alberta
  11. Green synthesis of various saturated S-heterocyclic scaffolds: an update
  12. Recent advances of heterocycle based anticancer hybrids
  13. Molecular docking and MD: mimicking the real biological process
  14. Synthesis of quinazolinone and quinazoline derivatives using green chemistry approach
  15. Nuclear fusion: the promise of endless energy
  16. Finance for Green Chemistry through Currency Mix
  17. Synthesis of bioactive scaffolds catalyzed by agro-waste-based solvent medium
  18. Recent developments in the green synthesis of biologically relevant cinnolines and phthalazines
  19. Detection of Rapid Eye Movement Behaviour Sleep Disorder using Time and Frequency Analysis of EEG Signal Applied on C4-A1 Channels
  20. Recent developments in C–C bond formation catalyzed by solid supported palladium: a greener perspective
  21. Visible-light-mediated metal-free C–Si bond formation reactions
  22. An overview of quinoxaline synthesis by green methods: recent reports
  23. Naturally occurring, natural product inspired and synthetic heterocyclic anti-cancer drugs
  24. Synthesis of bioactive natural products and their analogs at room temperature – an update
  25. One-pot multi-component synthesis of diverse bioactive heterocyclic scaffolds involving 6-aminouracil or its N-methyl derivatives as a versatile reagent
  26. Synthesis of new horizons in benzothiazole scaffold and used in anticancer drug development
  27. Triazine based chemical entities for anticancer activity
  28. Modification of kaolinite/muscovite clay for the removal of Pb(II) ions from aqueous media
  29. In silico design of ACE2 mutants for competitive binding of SARS-CoV-2 receptor binding domain with hACE2
  30. Computational study of Cu n AgAu (n = 1–4) clusters invoking DFT based descriptors
  31. Development of an online assessment system to evaluate knowledge on chemical safety and security
  32. Developing a questionnaire for diabetes mellitus type 2 risk effects and precondition factors – multivariate statistical paths
  33. Antioxidant and antibacterial activities of two xanthones derivatives isolated from the leaves extract of Anthocleista schweinfurthii Gilg (Loganiaceae)
  34. The stability increase of α-amylase enzyme from Aspergillus fumigatus using dimethyladipimidate
  35. Sustainability of ameliorative potentials of urea spiked poultry manure biochar types in simulated sodic soils
  36. Cytotoxicity test and antibacterial assay on the compound produced by the isolation and modification of artonin E from Artocarpus kemando Miq.
  37. Effects of alum, soda ash, and carbon dioxide on 40–50 year old concrete wastewater tanks
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