Isolation and characterization of regulators involved in PHOT1-mediated inhibition of hypocotyl phototropism in Arabidopsis

Qing-Ping Zhao; Xiang Zhao; Zi-Yi Zhu; Xi-ning Guo; Nan-Nan Li; Xiao Zhang

doi:10.1515/biolog-2017-0070

Article

Isolation and characterization of regulators involved in PHOT1-mediated inhibition of hypocotyl phototropism in Arabidopsis

Qing-Ping Zhao , Xiang Zhao , Zi-Yi Zhu , Xi-ning Guo , Nan-Nan Li and Xiao Zhang

Published/Copyright: June 30, 2017

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From the journal Biologia Volume 72 Issue 6

Abstract

Blue light receptor phot1 and phot2 regulate hypocotyl phototropism induced by high intensity blue light (HBL) in functional redundancy. The phenotype of rpt2 single mutant is similar with double mutant phot1phot2 that lost phototropism, but rpt2phot1 double mutant shows normal phototropic response, which indicating that phot1 has dual opposite function with inducing and inhibiting hypocotyls phototropism. However, the mechanism of phot1-mediated inhibition remains to be elucidated. Here, we screened mutants with phototropism from EMS mutagenesis of rpt2 and cloned the gene P1SA1 (Phototropin1Signaling Associated 1), which maybe locate downstream of phot1 and function in regulating phot1-mediated inhibitory response. The rpt2 p1sa1 mutant shows normal phototropism to unilateral HBL and overexpression of PHOT1 in rpt2 p1sa1 lost hypocotyl phototropism again, which indicated that P1SA1 may be the allelic of PHOT1. These results will open new perspectives about the screening and identification of genes involved in phot1-mediated inhibitory response, and provide theoretical basis for the self-regulation mechanism of plant to reduce high light damage.

Key words: Phot1; hypocotyls phototropism; EMS mutagenesis; map-based cloning

Acknowledgements

This work was supported by the National Natural Science Foundation of China (grant nos. 31670289 and 31570294 to X.Z.), Genetically Modified Organisms Breeding Major Projects of China (Grant no. 2016ZX08005–004) and Basic and Advanced Technology Research Project of Henan (Grant No. 142300413225).

We thank Dr. Ken-ichiro Shimazaki (Kyushu University, Japan) for providing the phot1, phot2, and phot1 phot2 mutants, Dr. Christian Fankhauser (University of Lausanne, Switzerland) for providing the rpt2-2, phot1 rpt2-2, phot2 rpt2-2 mutant seeds.

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Received: 2016-9-7

Accepted: 2017-2-15

Published Online: 2017-6-30

Published in Print: 2017-6-27

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Articles in the same Issue

https://doi.org/10.1515/biolog-2017-0070

Keywords for this article

Phot1; hypocotyls phototropism; EMS mutagenesis; map-based cloning