Forensic application of EST-derived STR markers in opium poppy
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Veronika Mičianová
Abstract
Discrimination study in opium poppy (Papaver somniferum L.) genotypes has been performed by short tandem repeat (STR) markers derived from expressed sequence tags (ESTs) retrieved from the opium poppy DNA. Some of STRs originate from the gene sequences involved in the biosynthesis of morphine. Eight of twelve tested EST-derived STR markers generated polymorphism, 2-4 alleles were identified per locus, and the mean polymorphic information content value was 0.338. Seven cultivars developed in Slovakia, registered and released at present time for commercial cultivation were discriminated mutually. All the Slovak and Czech cultivars were also different from each other. EST-derived STR markers have shown a potential for discrimination of opium poppy genotypes cultivated for commercial production of either edible seeds or pharmacologically active alkaloids and could be applicable for the PCR-based forensic analyses.
Acknowledgements
This work was supported by projects ITMS 26210120039 “Systems biology for protection, reproduction and use of plant resources of Slovakia” supported from the Operational Programme Research and Development co-financed from the European Regional Development Fund and APVV-16-0026 “Metagenomic approach for the identification and characterization of viral diseases in selected medicinal plant species” financed from the Slovak Research and Development Agency.
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Abbreviations
- AFLP
amplified fragment length polymorphism
- DI
diversity index
- EST
expressed sequence tag
- HE
expected heterozygosity
- HO
observed heterozygosity
- PCA
principal component analysis
- PI
probability of identity
- PIC
polymorphic information content
- SSR
simple sequence repeat
- STR
short tandem repeat
© 2017 Institute of Molecular Biology, Slovak Academy of Sciences
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- Cellular and Molecular Biology
- Forensic application of EST-derived STR markers in opium poppy
- Botany
- Phylogeny and maternal donor of Elymus (Triticeae: Poaceae) in China based on chloroplast matK sequences
- Botany
- Isolation and characterization of regulators involved in PHOT1-mediated inhibition of hypocotyl phototropism in Arabidopsis
- Botany
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- Botany
- Salt stress resilience potential of a fungal inoculant isolated from tea cultivation area in maize
- Zoology
- Distribution of the genus Veigaia (Mesostigmata: Veigaiidae) in Romania with notes on the species ecology
- Zoology
- The effect of grassland management on diversity and composition of ground-dwelling spider assemblages in the Mátra Landscape Protection Area of Hungary
- Zoology
- Downstream effect of a pumped-storage hydropower plant on river habitat conditions and benthic life – a case study
- Zoology
- Parasites as biological tags of divergence in Central European gudgeon populations (Actinopterygii: Cyprinidae: Gobioninae)
- Zoology
- Chromosomal characteristics of rDNA in a conserved karyotype of two Sternopygus macrurus (Gymnotiformes: Sternopygidae) populations from upper Paraná River basin
- Zoology
- Habitat suitability and patterns of sex-biased migration of the Iranian long-legged wood frog, Rana pseudodalmatina (Anura: Ranidae)
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- Blebbistatin induces chondrogenesis of single mesenchymal cells via PI3K/PDK1/mTOR/p70S6K pathway
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