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Certification of the Mass Concentration of Creatine Kinase Isoenzyme 2 (CK-MB) in the Reference Material BCR 608
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Maribel Sánchez
Published/Copyright:
June 1, 2005
Abstract
We describe the certification of a mass concentration value in the already prepared creatine kinase-2 reference material (BCR 608). Creatine kinase-2 was purified from human heart. The purified enzyme was diluted in order to measure its protein concentration by the Doetsch method. A protein concentration value of 124.30 ± 13.17 mg/l was assigned to the stock solution of purified creatine kinase-2. This stock solution was diluted in 25 mmol/l piperazine-N,N′-bis[2-ethanesulfonic acid] (PIPES) pH 7.2, containing 2 mmol/l ADP, 5 mmol/l 2-mercaptoethanol, 154 mmol/l sodium chloride and 50 g/l human albumin to obtain a stable liquid standard of known creatine kinase-2 mass concentration (80.36 μg/l). This standard was then used to recalculate the creatine kinase-2 mass concentration measured in the BCR 608 material by immunoassay. The mass concentration of creatine kinase-2 in samples of reconstituted BCR 608 was certified to be 93.30 ± 9.65 μg/l.
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Published Online: 2005-06-01
Published in Print: 2001-09-07
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
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- Herbert Keller January 16, 1925 June 25, 2001
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- Diagnostic Testing in Epstein-Barr Virus Infection
- Establishing a Reference System in Clinical Enzymology
- Prediction of the Development of Pregnancy-Induced Hypertensive Disorders in High-Risk Pregnant Women by Artificial Neural Networks
- Fast Homogeneous Assay for Plasma Procarboxypeptidase U
- Detection and Quantification of Small Numbers of Circulating Tumour Cells in Peripheral Blood Using Laser Scanning Cytometer (LSC®)
- Monotherapy with Metformin: Does It Improve Hypoxia in Type 2 Diabetic Patients?
- Cigarette Smoking and Hypertension Influence Nitric Oxide Release and Plasma Levels of Adhesion Molecules
- The Effect of Corticosteroids on Serum and Cerebrospinal Fluid Nitric Oxide Levels in Multiple Sclerosis
- Glutathione Transferase P1 Polymorphism in Neuroblastoma Studied by Endonuclease Restriction Mapping
- Biological Variation of Interleukin-1β, Interleukin-8 and Tumor Necrosis Factor-α in Serum of Healthy Individuals
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