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Routine Differential Diagnosis of Proteinurias by Capillary Electrophoresis

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Published/Copyright: June 1, 2005
Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Volume 39 Issue 9

Abstract

Capillary electrophoresis is a relatively new analytical technique that begins to have an impact on both routine and research in clinical laboratories. Recently, a fully automated system has become commercially available (Paragon CZE 2000, Beckman, USA) for the analysis of human serum proteins. Urine protein analysis, on the other hand, is currently accomplished by electrophoresis of concentrated urine specimens. The method is used to distinguish the glomerular from the tubular proteinuria and for the identification of Bence-Jones proteins. The procedure is labor-intensive and technically demanding. We developed a technique for the serum capillary electrophoresis instrument that can also be applied routinely to the differential diagnosis of proteinurias. Overriding the programmed dilution step of the instrument, we were able to distinguish different types of proteinurias without concentration of specimens with a total protein content of 150–200 mg/l as determined by sulfosalicylic acid. The different electrophoretic patterns obtained by the capillary electrophoresis system for various specimens correlated well with established techniques (Hydragel Proteinurie Kit, Sebia, France). The method is applicable for routine analysis of urinary proteins. It is reliable, less expensive and faster than the conventional methods (electrophoretic or immunonephelometric) used today for the differentiation of proteinurias, and it can be used as a quick screening test.

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Published Online: 2005-06-01
Published in Print: 2001-09-07

Copyright © 2001 by Walter de Gruyter GmbH & Co. KG

Articles in the same Issue

  1. Joint Meeting of the German Society of Clinical Chemistry and of the German Society of Laboratory Medicine, Rostock, 1619 September 2001
  2. Herbert Keller January 16, 1925 June 25, 2001
  3. Routine Differential Diagnosis of Proteinurias by Capillary Electrophoresis
  4. Diagnostic Testing in Epstein-Barr Virus Infection
  5. Establishing a Reference System in Clinical Enzymology
  6. Prediction of the Development of Pregnancy-Induced Hypertensive Disorders in High-Risk Pregnant Women by Artificial Neural Networks
  7. Fast Homogeneous Assay for Plasma Procarboxypeptidase U
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  9. Monotherapy with Metformin: Does It Improve Hypoxia in Type 2 Diabetic Patients?
  10. Cigarette Smoking and Hypertension Influence Nitric Oxide Release and Plasma Levels of Adhesion Molecules
  11. The Effect of Corticosteroids on Serum and Cerebrospinal Fluid Nitric Oxide Levels in Multiple Sclerosis
  12. Glutathione Transferase P1 Polymorphism in Neuroblastoma Studied by Endonuclease Restriction Mapping
  13. Biological Variation of Interleukin-1β, Interleukin-8 and Tumor Necrosis Factor-α in Serum of Healthy Individuals
  14. Reference Intervals and Variation for Urinary Epinephrine, Norepinephrine and Cortisol in Healthy Men and Women in Denmark
  15. Determination of Serum Cystatin C: Biological Variation and Reference Values
  16. Certification of the Mass Concentration of Creatine Kinase Isoenzyme 2 (CK-MB) in the Reference Material BCR 608
  17. Carbohydrate-Deficient Transferrin (CDT) Determination by Nephelometry Using a Commercial Kit. Analytical and Diagnostic Aspects
  18. Higher CK-MB Mass Values in Heparin Plasma than in Serum Measured with the Abbott AxSYM™ System
  19. Clinical Case Material for Teaching Clinical Chemistry and Laboratory Medicine
  20. IFCC News
  21. Meetings
https://doi.org/10.1515/CCLM.2001.129

Articles in the same Issue

  1. Joint Meeting of the German Society of Clinical Chemistry and of the German Society of Laboratory Medicine, Rostock, 1619 September 2001
  2. Herbert Keller January 16, 1925 June 25, 2001
  3. Routine Differential Diagnosis of Proteinurias by Capillary Electrophoresis
  4. Diagnostic Testing in Epstein-Barr Virus Infection
  5. Establishing a Reference System in Clinical Enzymology
  6. Prediction of the Development of Pregnancy-Induced Hypertensive Disorders in High-Risk Pregnant Women by Artificial Neural Networks
  7. Fast Homogeneous Assay for Plasma Procarboxypeptidase U
  8. Detection and Quantification of Small Numbers of Circulating Tumour Cells in Peripheral Blood Using Laser Scanning Cytometer (LSC®)
  9. Monotherapy with Metformin: Does It Improve Hypoxia in Type 2 Diabetic Patients?
  10. Cigarette Smoking and Hypertension Influence Nitric Oxide Release and Plasma Levels of Adhesion Molecules
  11. The Effect of Corticosteroids on Serum and Cerebrospinal Fluid Nitric Oxide Levels in Multiple Sclerosis
  12. Glutathione Transferase P1 Polymorphism in Neuroblastoma Studied by Endonuclease Restriction Mapping
  13. Biological Variation of Interleukin-1β, Interleukin-8 and Tumor Necrosis Factor-α in Serum of Healthy Individuals
  14. Reference Intervals and Variation for Urinary Epinephrine, Norepinephrine and Cortisol in Healthy Men and Women in Denmark
  15. Determination of Serum Cystatin C: Biological Variation and Reference Values
  16. Certification of the Mass Concentration of Creatine Kinase Isoenzyme 2 (CK-MB) in the Reference Material BCR 608
  17. Carbohydrate-Deficient Transferrin (CDT) Determination by Nephelometry Using a Commercial Kit. Analytical and Diagnostic Aspects
  18. Higher CK-MB Mass Values in Heparin Plasma than in Serum Measured with the Abbott AxSYM™ System
  19. Clinical Case Material for Teaching Clinical Chemistry and Laboratory Medicine
  20. IFCC News
  21. Meetings
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