Startseite In vitro antibacterial activities, DPPH radical scavenging, and molecular simulation of isolated compounds from the leaves of Rhus ruspolii
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In vitro antibacterial activities, DPPH radical scavenging, and molecular simulation of isolated compounds from the leaves of Rhus ruspolii

  • Tolessa Duguma , Yadessa Melaku EMAIL logo , Daniel Rentsch , Akalu Terfa und Kebede Shenkute
Veröffentlicht/Copyright: 23. September 2024
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Zeitschrift für Naturforschung C
Aus der Zeitschrift Zeitschrift für Naturforschung C Band 80 Heft 5-6

Abstract

Rhus ruspolii Engl. plant is traditionally used in Ethiopia to treat various diseases. However, the biological and phytochemical properties of the leaves are not well documented. Hence, this study aimed to isolate phytochemicals from R. ruspolii leaves and evaluate their antibacterial and DPPH radical scavenging activities. GC-MS analysis identified 16 compounds from combined fractions 6–10. Chromatographic separation and NMR analysis resulted in the isolation and characterization of palmitic acid (7), 3,4-dihydroxybenzoic acid (17), cupressuflavone (18), amentoflavone (19), shikimic acid (20), avicularin (21), and myricetin-3-O-5′′-acetylarabinofuranoside (22). The inhibition zones of extracts (100 mg/mL) and isolated compounds (5 mg/mL) ranged from 8.33 ± 0.50 to 16.33 ± 0.47 mm against all evaluated bacteria. Of all isolated compounds, compounds 18 and 21 showed good activity against Gram-negative (supported by in silico molecular docking studies) and Gram-positive bacteria, respectively. The lowest (49.1 %) and the highest (91.3 %) DPPH radicals were inhibited by combined fractions 6–10 and compound 17, respectively, at 62.5 μg/mL. The SwissADME online analysis showed compounds 17 and 20 have good solubility and permeability. The Pro Tox 3.0 online analysis revealed none of the isolated compounds are fatal if swallowed. Therefore, the findings of this study support the traditional use of the plant for treating bacteria diseases.

Keywords: R. ruspolii ; phytochemicals; antibacterial; DPPH radical; docking
Corresponding author: Yadessa Melaku, Department of Applied Chemistry, Adama Science and Technology University, P.O. Box: 1888, Adama, Ethiopia, E-mail:

Acknowledgments

The authors acknowledge Adama Science and Technology University for financial and material support and Mr. Muktar Ahmed for conducting antibacterial activity.

  1. Research ethics: Not applicable.

  2. Author contributions: TD planned the study, carried out the investigation, analyzed, interpreted the data, wrote, and edited the original draft of the manuscript. YM planned the study, supervised, analyzed, interpreted the data, reviewed, and edited the manuscript. DR recorded, interpreted NMR spectral data, and reviewed the manuscript. AT planned and supervised the study. KS conducted GC-MS analysis.

  3. Use of Large Language Models, AI and Machine Learning Tools: None declared.

  4. Conflict of interest: The authors state no conflict of interest.

  5. Research funding: Not declared.

  6. Data availability: All data generated or analyzed during this study are included in this published article and provided in supplementary material files.

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Supplementary Material

This article contains supplementary material (https://doi.org/10.1515/znc-2024-0127).

Received: 2024-05-29
Accepted: 2024-09-05
Published Online: 2024-09-23
Published in Print: 2025-05-26

© 2024 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/znc-2024-0127
Schlagwörter für diesen Artikel
R. ruspolii; phytochemicals; antibacterial; DPPH radical; docking

Artikel in diesem Heft

  1. Frontmatter
  2. Review Articles
  3. Nourishment beyond grains: unveiling the multifaceted contributions of millets to United Nations Sustainable Development Goals
  4. Exploring the functionality of fluorescent liposomes in cancer: diagnosis and therapy
  5. Research Articles
  6. In vitro antibacterial activities, DPPH radical scavenging, and molecular simulation of isolated compounds from the leaves of Rhus ruspolii
  7. Characterization and antimicrobial activity of essential oils extracted from lemongrass (Cymbopogon flexuosus) using microwave-assisted hydro distillation
  8. In silico DFT and molecular modeling of novel pyrazine-bearing thiazolidinone hybrids derivatives: elucidating in vitro anti-cancer and urease inhibitors
  9. Molecular mechanisms of the anticancer action of fustin isolated from Cotinus coggygria Scop. in MDA-MB-231 triple-negative breast cancer cell line
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Heruntergeladen am 6.11.2025 von https://www.degruyterbrill.com/document/doi/10.1515/znc-2024-0127/html
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