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Spermidine protects cellular redox status and ionic homeostasis in D-galactose induced senescence and natural aging rat models

  • Sandeep Singh ORCID logo , Avnish Kumar Verma ORCID logo , Geetika Garg ORCID logo , Abhishek Kumar Singh ORCID logo and Syed Ibrahim Rizvi ORCID logo EMAIL logo
Published/Copyright: October 24, 2024
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Zeitschrift für Naturforschung C
From the journal Zeitschrift für Naturforschung C Volume 80 Issue 5-6

Abstract

Impaired redox homeostasis is an important hallmark of aging. Among various anti-aging interventions, caloric restriction mimetics (CRMs) are the most effective in promoting health and longevity. The potential role of spermidine (SPD) as a CRM in modulating oxidative stress and redox homeostasis during aging remains unclear. This study aimed to investigate the protective effect of SPD in D-galactose (D-gal) accelerated induced senescence model and naturally aged rats. Young male rats (4 months), D-gal induced (500 mg/kg b. w., subcutaneously) aging model and naturally aged (22 months) rats were supplemented with SPD (10 mg/kg b. w., orally) for 6 weeks. The results showed that SPD supplementation suppresses the age induced increase in reactive oxygen species, lipid peroxidation and protein oxidation. Additionally, it increases the level of antioxidants, plasma membrane redox system in erythrocytes and membrane. These results also indicate that membrane transporter activity is correlated with the susceptibility of the erythrocyte towards oxidative damage. We therefore present evidence that SPD improves redox status and membrane impairments in erythrocytes in experimental and naturally aging rat models, however, more research is required to recommend a potential therapeutic role for SPD as an anti-aging intervention strategy.

Keywords: aging; erythrocytes; membrane transporters; oxidative stress; spermidine
Corresponding author: Syed Ibrahim Rizvi, Department of Biochemistry, University of Allahabad, Allahabad, 211002, India, E-mail:

Funding source: Department of Biotechnology, Ministry of Science and Technology, India

Award Identifier / Grant number: RRFSP

Acknowledgments

Authors gratefully acknowledge for the funding and facilities received by Department of Biochemistry, University of Allahabad, India.

  1. Research ethics: The experimental protocol was approved by the Institutional Animal Ethics committee (IAEC), University of Allahabad, Allahabad, and the experiments were conducted in compliance with the guidelines of the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), India.

  2. Informed consent: Not applicable.

  3. Author contributions: SS: Conceptualization of the study, performed experiments and data collection. AVK and GG: Experimental work and figures preparation. AKS: Data analysis, data interpretation and manuscript draft preparation. SIR: Conceptualization of study design, manuscript editing and finalized for publication.

  4. Use of Large Language Models, AI and Machine Learning Tools: None declared.

  5. Conflict of interests: The authors state no conflict of interest.

  6. Research funding: This work was also supported by a research grant to SIR from DBT, Govt of India (RRFSP). AKV is recipient of Council of Scientific and Industrial Research (CSIR), New Delhi, India. The Department of Biochemistry is supported by FIST grant of DST, New Delhi, and SAP DRS I from UGC, India.

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Received: 2024-08-23
Accepted: 2024-10-05
Published Online: 2024-10-24
Published in Print: 2025-05-26

© 2024 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/znc-2024-0181
Keywords for this article
aging; erythrocytes; membrane transporters; oxidative stress; spermidine

Articles in the same Issue

  1. Frontmatter
  2. Review Articles
  3. Nourishment beyond grains: unveiling the multifaceted contributions of millets to United Nations Sustainable Development Goals
  4. Exploring the functionality of fluorescent liposomes in cancer: diagnosis and therapy
  5. Research Articles
  6. In vitro antibacterial activities, DPPH radical scavenging, and molecular simulation of isolated compounds from the leaves of Rhus ruspolii
  7. Characterization and antimicrobial activity of essential oils extracted from lemongrass (Cymbopogon flexuosus) using microwave-assisted hydro distillation
  8. In silico DFT and molecular modeling of novel pyrazine-bearing thiazolidinone hybrids derivatives: elucidating in vitro anti-cancer and urease inhibitors
  9. Molecular mechanisms of the anticancer action of fustin isolated from Cotinus coggygria Scop. in MDA-MB-231 triple-negative breast cancer cell line
  10. Green synthesized AgNPs of the Anchusa arvensis aqueous extract resulting in impressive protein kinase, antioxidant, antibacterial, and antifungal activities
  11. Phytochemical composition, antimicrobial, antioxidant, and wound healing activities of Thermopsis turcica
  12. Molecular detection of Coxiella burnetii infection (Q fever) in livestock in Makkah Province, Saudi Arabia
  13. Spermidine protects cellular redox status and ionic homeostasis in D-galactose induced senescence and natural aging rat models
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