Gel filtration chromatography analysis and modeling the process of pullulan depolymerization
-
Ivan M. Savic
,
Goran S. Nikolic
Abstract
Different fractions of pullulan oligosaccharide can be applied in the synthesis of pharmaceutical active compounds. Thus, the object of this study was to model the depolymerization process of pullulan polysaccharide with the change of pH, temperature and depolymerization time. The samples were analyzed by gel filtration chromatography (GFC). The chromatography separation of polymer fractions was achieved by using a Zorbax PSM-300 HP-SEC column (250×6.2 mm, 5 μm) at a temperature of 25°C. The flow rate of the mobile phase (redistilled water) was 1 cm3 min-1. The fractions were recorded by a refractive index detector. The obtained data were converted by thin plate spline interpolation function into matrixes. By the mathematical processing matrixes, the models of molar mass distribution in the function of pH, temperature and depolymerization time were obtained. The intervals of depolymerization parameters can be defined for obtaining the desirable pullulan oligomers, which have the efficient applications in macromolecule technology.
©2012 by Walter de Gruyter Berlin Boston
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Artikel in diesem Heft
- Masthead
- Masthead
- Masthead
- Original Articles
- Evaluation of factors and kinetics study of polyacrylamide redox polymerization using statistical design modeling
- Gel filtration chromatography analysis and modeling the process of pullulan depolymerization
- Polystyrene–organoclay nanocomposites produced by in situ activators regenerated by electron transfer for atom transfer radical polymerization
- Viscoelasticity in thermoforming
- Melt extrudate swell behavior of polypropylene composites filled with hollow glass beads
- Viscosity and domain morphology in binary immiscible blends of poly(trimethylene terephthalate) and polyamide6,10
- Effect of poly (methyl methacrylate)-grafted-talc content on mechanical properties and thermal degradation of poly (vinyl chloride) composites
- Fabrication and properties of poly(L-lactide)/hydroxyapatite/chitosan fiber ternary composite scaffolds for bone tissue engineering
- Green composites based on recycled polyamide-6/recycled polypropylene kenaf composites: mechanical, thermal and morphological properties
- Effect of gamma radiation and bulk monomer on jute fabrics polyethylene/polyvinyl chloride composites
- Investigation of fracture toughness parameters of epoxy nanocomposites for different crack angles
- Influence of X-ray opaque BaSO4 nanoparticles on the mechanical, thermal and rheological properties of polyoxymethylene nanocomposites
