Startseite Host–parasite relationship modulates the effect of African mistletoe leaves on the cholinergic, monoaminergic and carbohydrate hydrolyzing enzymes in fruit fly
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Host–parasite relationship modulates the effect of African mistletoe leaves on the cholinergic, monoaminergic and carbohydrate hydrolyzing enzymes in fruit fly

  • Olubukola H. Oyeniran EMAIL logo , Adedayo O. Ademiluyi und Ganiyu Oboh
Veröffentlicht/Copyright: 22. Juni 2021
Journal of Basic and Clinical Physiology and Pharmacology
Aus der Zeitschrift Journal of Basic and Clinical Physiology and Pharmacology Band 34 Heft 5

Abstract

Objectives

Mistletoe infests common plant trees of great medicinal values such as Moringa and Almond. According to folklore, mistletoe leaves have been found to have application as food and medicine in the alleviation of various degenerative diseases. Host–parasite relationship may possibly influence the phytochemical and biological activities of mistletoe leaves. Hence, we examined the polyphenol contents, antioxidant properties, α-amylase, α-glucosidase, acetylcholinesterase (AChE) and monoamine oxidase (MAO) inhibitory activities of African mistletoe leaves obtained from Moringa and Almond host plants in fruit fly in vitro.

Methods

The phenolic constituents of the leaves were evaluated using HPLC system. The antioxidant activities were determined through the ABTS, DPPH and OH free radicals scavenging properties, ferric (Fe3+) and malondialdehyde (MDA) reducing abilities and Fe2+ chelation. The inhibitory effects of the leaves aqueous extracts on α-amylase, α-glucosidase, AChE and MAO activities were also assessed.

Results

The HPLC characterization of the leaves revealed that host plants caused marked variation in their phenolic composition, however, Almond mistletoe leaves had significantly (p<0.05) greater amounts of phenolic constituents. Both Moringa and Almond mistletoe leaves reduced Fe3+ and MDA levels, scavenged free radicals, chelated Fe2+ and inhibited α-amylase, α-glucosidase, AChE and MAO activities with the Almond mistletoe leaves having significantly (p<0.05) higher antioxidant properties and enzyme inhibitory activities.

Conclusions

This present study indicated that host plants could positively modulate the phenolic profile of mistletoe leaves and this probably brought about the vivid noticeable changes in their antioxidant abilities, cholinergic, monoaminergic and carbohydrate hydrolyzing enzymes inhibitory activities.

Keywords: antioxidant; enzymes; fruit fly; mistletoe leaves; Moringa and Almond host plants; phytochemistry
Corresponding author: Olubukola H. Oyeniran, Department of Biochemistry, Functional Foods, Nutraceuticals and Phytomedicine Unit, Federal University of Technology, 340001, Akure, Nigeria; and Department of Biochemistry, Federal University, P.M.B. 373, Oye-Ekiti, Ekiti State, Nigeria, Phone: +234 8062686571, E-mail:

Acknowledgments

Not applicable.

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethical approval: The local Institutional Review Board deemed the study exempt from review. The use of fruit fly as a model for research is exempted from regulations pertaining to ethical approvals and/or animal protection laws. However, the use of the fruit fly as a model organism is in compliance with the 3Rs protocol of the European Centre for the Validation of Alternative Methods on 3Rs – refinement, replacement and reduction.

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Received: 2020-09-20
Accepted: 2021-04-15
Published Online: 2021-06-22

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Editorials
  3. Deiodination and tumor progression: the interplay between thyroid hormones intracellular activation and the androgen signal
  4. The intricate role of glutamine in pathophysiological contexts
  5. Reviews
  6. Zebrafish as a model organism – can a fish mimic human?
  7. Phytocompounds and their molecular targets in immunomodulation: a review
  8. Original Articles
  9. Host–parasite relationship modulates the effect of African mistletoe leaves on the cholinergic, monoaminergic and carbohydrate hydrolyzing enzymes in fruit fly
  10. Behavioral and biochemical investigations to explore the efficacy of quercetin and folacin in experimental diabetes induced vascular endothelium dysfunction and associated dementia in rats
  11. Chronic exposure of industrial grade calcium carbide and ethylene glycol exert genotoxic effect in Wistar albino rats
  12. A four-year review of uterine rupture at a secondary health facility in Okitipupa, Southwest Nigeria
  13. Increased nitric oxide availability worsens the cardiac performance during early re-perfusion period in adult rats
  14. Safety evaluation of an antimalarial herbal product from Andrographis paniculata (AS201-01) in healthy volunteers
  15. Tobacco use and clinical leukoplakia lesions among south Indian tribes
  16. Carcinogen sodium arsenite disrupts antioxidant and redox homeostasis in Drosophila melanogaster
  17. No association of the common Asian mitochondrial DNA haplogroups with lung cancer in East Indian population
  18. Cardioprotective effect of Justicia gendarussa on doxorubicin induced toxicity in mice
  19. Lung ultrasound-guided PEEP titration in COVID–19 patients treated with CPAP
  20. Short Communication
  21. Effects of the gaseous signalling molecule nitroxyl (HNO) on myenteric neurons governing intestinal motility
https://doi.org/10.1515/jbcpp-2020-0298
Schlagwörter für diesen Artikel
antioxidant; enzymes; fruit fly; mistletoe leaves; Moringa and Almond host plants; phytochemistry

Artikel in diesem Heft

  1. Frontmatter
  2. Editorials
  3. Deiodination and tumor progression: the interplay between thyroid hormones intracellular activation and the androgen signal
  4. The intricate role of glutamine in pathophysiological contexts
  5. Reviews
  6. Zebrafish as a model organism – can a fish mimic human?
  7. Phytocompounds and their molecular targets in immunomodulation: a review
  8. Original Articles
  9. Host–parasite relationship modulates the effect of African mistletoe leaves on the cholinergic, monoaminergic and carbohydrate hydrolyzing enzymes in fruit fly
  10. Behavioral and biochemical investigations to explore the efficacy of quercetin and folacin in experimental diabetes induced vascular endothelium dysfunction and associated dementia in rats
  11. Chronic exposure of industrial grade calcium carbide and ethylene glycol exert genotoxic effect in Wistar albino rats
  12. A four-year review of uterine rupture at a secondary health facility in Okitipupa, Southwest Nigeria
  13. Increased nitric oxide availability worsens the cardiac performance during early re-perfusion period in adult rats
  14. Safety evaluation of an antimalarial herbal product from Andrographis paniculata (AS201-01) in healthy volunteers
  15. Tobacco use and clinical leukoplakia lesions among south Indian tribes
  16. Carcinogen sodium arsenite disrupts antioxidant and redox homeostasis in Drosophila melanogaster
  17. No association of the common Asian mitochondrial DNA haplogroups with lung cancer in East Indian population
  18. Cardioprotective effect of Justicia gendarussa on doxorubicin induced toxicity in mice
  19. Lung ultrasound-guided PEEP titration in COVID–19 patients treated with CPAP
  20. Short Communication
  21. Effects of the gaseous signalling molecule nitroxyl (HNO) on myenteric neurons governing intestinal motility
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