Increased nitric oxide availability worsens the cardiac performance during early re-perfusion period in adult rats
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Faten M.A. Diab
Abstract
Objectives
Re-perfusion is the standard therapy for acute myocardial infarction, despite the associated pathologies that may contribute to irreversible myocardial injury. The present study aims to clarify the alterations in cardiac activities in response to experimental cardiac ischemic arrest followed by re-perfusion in isolated hearts perfused with nitric oxide (NO) donor, l-arginine, or NO inhibitor, Nω-Nitro-l-arginine methyl ester hydrochloride (l-NAME), to shed light on the possible role of NO in the re-perfusion process.
Methods
Hearts isolated from adult Wistar rats were studied on Langendorff preparation under basal conditions and during 30 min re-perfusion following 30 min of total global ischemia. Rats were randomly divided into three groups; control and l-arginine or l-NAME infused heart groups. Cardiac tissue content of malondialdhyde, catalase and nitrite was also measured.
Results
Compared to the control group, both l-arginine and l-NAME infused hearts showed increased basal chronotropy and myocardial flow rate. Following ischemia and during the whole period of re-perfusion, the three groups demonstrated significant deterioration in the inotropic activity and compromised myocardial flow rate. l-arginine infused hearts revealed depressed inotropy and chronotropy, weak systolic and diastolic functions with compromised myocardial flow at early 5 min of re-perfusion, yet with significantly higher myocardial flow rate by the end of re-perfusion.
Conclusions
Reducing NO availability by l-NAME revealed mild impact on the ischemia re-perfusion induced contractile dysfunction, whereas excess NO worsens cardiac performance at the early re-perfusion period.
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Research funding: None declared.
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Author contribution: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Informed consent: Not applicable.
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Ethical approval: All the experimental procedures were carried out in accordance with Guide for the Care and Use of Laboratory Animals and approved by the Research Ethics Committee (REC), Faculty of Medicine, Ain Shams University (protocol number FMASU 277/2015).
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© 2021 Walter de Gruyter GmbH, Berlin/Boston
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Articles in the same Issue
- Frontmatter
- Editorials
- Deiodination and tumor progression: the interplay between thyroid hormones intracellular activation and the androgen signal
- The intricate role of glutamine in pathophysiological contexts
- Reviews
- Zebrafish as a model organism – can a fish mimic human?
- Phytocompounds and their molecular targets in immunomodulation: a review
- Original Articles
- Host–parasite relationship modulates the effect of African mistletoe leaves on the cholinergic, monoaminergic and carbohydrate hydrolyzing enzymes in fruit fly
- Behavioral and biochemical investigations to explore the efficacy of quercetin and folacin in experimental diabetes induced vascular endothelium dysfunction and associated dementia in rats
- Chronic exposure of industrial grade calcium carbide and ethylene glycol exert genotoxic effect in Wistar albino rats
- A four-year review of uterine rupture at a secondary health facility in Okitipupa, Southwest Nigeria
- Increased nitric oxide availability worsens the cardiac performance during early re-perfusion period in adult rats
- Safety evaluation of an antimalarial herbal product from Andrographis paniculata (AS201-01) in healthy volunteers
- Tobacco use and clinical leukoplakia lesions among south Indian tribes
- Carcinogen sodium arsenite disrupts antioxidant and redox homeostasis in Drosophila melanogaster
- No association of the common Asian mitochondrial DNA haplogroups with lung cancer in East Indian population
- Cardioprotective effect of Justicia gendarussa on doxorubicin induced toxicity in mice
- Lung ultrasound-guided PEEP titration in COVID–19 patients treated with CPAP
- Short Communication
- Effects of the gaseous signalling molecule nitroxyl (HNO) on myenteric neurons governing intestinal motility
