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Human carbonic anhydrase VII protects cells from oxidative damage

  • Rita Del Giudice , Daria M. Monti EMAIL logo , Emanuela Truppo , Angela Arciello , Claudiu T. Supuran , Giuseppina De Simone und Simona M. Monti EMAIL logo
Veröffentlicht/Copyright: 12. Juli 2013

Abstract

Human carbonic anhydrase (hCA) VII is a cytosolic enzyme with high carbon dioxide hydration activity. Recently, S-glutathionylation of two cysteine residues from the enzyme was revealed, suggesting a new role as oxygen radical scavenger. We analyzed the effect of native and tetramutated hCA VII (all cysteines mutated into serines) in a eukaryotic system by stressing cells with an oxidant agent. Results clearly show that native hCA VII can protect cells from oxidative damage by preventing the apoptosis cascade and that cysteines play a leading role in this process. Our findings definitively confirm hCA VII protective role toward oxidative insult.


Corresponding authors: Daria M. Monti, Department of Chemical Sciences, University of Naples Federico II, I-80126 Naples, Italy; and Simona M. Monti, Institute of Biostructures and Bioimaging-CNR, Via Mezzocannone 16, I-80134 Naples, Italy

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Received: 2013-6-14
Accepted: 2013-7-9
Published Online: 2013-07-12
Published in Print: 2013-10-01

©2013 by Walter de Gruyter Berlin Boston

Heruntergeladen am 15.1.2026 von https://www.degruyterbrill.com/document/doi/10.1515/hsz-2013-0204/html
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