Early-life stress altered pancreatic Krebs cycle-related enzyme activities in response to young adulthood physical and psychological stress in male rat offspring
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Mina Salimi
Abstract
Objectives
Early-life stress (ELS) increases the risk of metabolic disorders in later life. The present study investigated the ELS effect on pancreatic pyruvate dehydrogenase (PDH) protein level, α-ketoglutarate dehydrogenase (α-KGDH), and aconitase activities as metabolic enzymes in response to young adulthood stress in male rat offspring.
Methods
Male Wistar rats were divided into six groups: Control, early life stress (Early STR), young adult foot-shock stress (Y. adult F-SH STR), early + young adult foot-shock stress (Early + Y. adult F-SH STR), young adult psychological stress (Y. adult Psy STR) and early + young adult psychological stress (Early + Y. adult Psy STR). Stress was induced by a communication box at 2 weeks of age and young adulthood for five consecutive days. The blood samples were collected in young adult rats, then pancreases were removed to measure its PDH protein level and aconitase and α-KGDH activities.
Results
In ELS animals, applying foot-shock stress in young adulthood increased PDH protein level, decreased α-KGDH and aconitase activities, and increased plasma glucose, insulin, and corticosterone concentrations. However, exposure to young adulthood psychological stress only decreased α-KGDH and aconitase activities.
Conclusions
It seems that ELS altered metabolic response to young adulthood stress through changes of Krebs cycle-related enzymes activities, though the type of adulthood stress was determinant.
Funding source: Research Deputy of School of Medicine
Award Identifier / Grant number: 15314
Funding source: Shahid Beheshti University of Medical Sciences
Research funding: This study was supported by a grant (No.:15314) from Research Deputy of School of Medicine, Shahid Beheshti University of Medical Sciences.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: The authors declare that they have no conflict of interest.
Informed consent: Not applicable.
Ethical approval: All procedures were approved by the Animal Care and Use Committee of the Neurophysiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran (IR.SBMU.MSP.REC.1397.680).
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© 2020 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Letter to the Editor
- Kerala model for combating COVID-19 pandemic
- Original Articles
- Evaluation of the potential anticancer activity of different vitamin D metabolites on colorectal and breast cancer cell lines
- Relationship between the levels of serum fibroblast growth factor 19 and metabolic factors in obese and normal weight subjects with and without type 2 diabetes mellitus: a case-control study
- Early-life stress altered pancreatic Krebs cycle-related enzyme activities in response to young adulthood physical and psychological stress in male rat offspring
- Effect of short-term and long-term traffic noise exposure on the thyroid gland in adult rats: a sexual dimorphic study
- The effect of thyroid dysfunction and treatment on adropin, asprosin and preptin levels in rats
- Comparison of the effects of zinc oxide and zinc oxide nanoparticles on the expression of hepcidin gene in rat liver
- Comparison between Iberet Folic® and Zincofer® in treatment of iron deficiency anaemia in pregnancy
- Mini Review
- COVID-19 and pregnancy: are they friends or enemies?
- Review Articles
- COVID-19: a review on SARS-CoV-2 origin, epidemiology, virology, clinical manifestations and complications with special emphasis on adverse outcome in Bhopal Gas Tragedy survivor
- COVID-19 and neurology perspective
- COVID-19 and nutritional deficiency: a review of existing knowledge
- COVID-19 and geriatric population: from pathophysiology to clinical perspectives
- COVID-19 and cardiovascular disease: a review of current knowledge
- How do different histologic components of mixed endometrial carcinomas affect prognosis? Does it really matter?
Artikel in diesem Heft
- Frontmatter
- Letter to the Editor
- Kerala model for combating COVID-19 pandemic
- Original Articles
- Evaluation of the potential anticancer activity of different vitamin D metabolites on colorectal and breast cancer cell lines
- Relationship between the levels of serum fibroblast growth factor 19 and metabolic factors in obese and normal weight subjects with and without type 2 diabetes mellitus: a case-control study
- Early-life stress altered pancreatic Krebs cycle-related enzyme activities in response to young adulthood physical and psychological stress in male rat offspring
- Effect of short-term and long-term traffic noise exposure on the thyroid gland in adult rats: a sexual dimorphic study
- The effect of thyroid dysfunction and treatment on adropin, asprosin and preptin levels in rats
- Comparison of the effects of zinc oxide and zinc oxide nanoparticles on the expression of hepcidin gene in rat liver
- Comparison between Iberet Folic® and Zincofer® in treatment of iron deficiency anaemia in pregnancy
- Mini Review
- COVID-19 and pregnancy: are they friends or enemies?
- Review Articles
- COVID-19: a review on SARS-CoV-2 origin, epidemiology, virology, clinical manifestations and complications with special emphasis on adverse outcome in Bhopal Gas Tragedy survivor
- COVID-19 and neurology perspective
- COVID-19 and nutritional deficiency: a review of existing knowledge
- COVID-19 and geriatric population: from pathophysiology to clinical perspectives
- COVID-19 and cardiovascular disease: a review of current knowledge
- How do different histologic components of mixed endometrial carcinomas affect prognosis? Does it really matter?
