The effect of thyroid dysfunction and treatment on adropin, asprosin and preptin levels in rats
-
Rasim Mogulkoc
,
Dervis Dasdelen
Abstract
Objectives
Thyroid hormones have important roles in normal development and energy regulating mechanisms as well as signaling mechanisms that affect energy consumption through central and peripheral pathways. The aim of this study was to determine the effects of thyroid dysfunction on adropin, asprosin and preptin levels in rat.
Methods
The study was performed on the 38 male Wistar-albino rats. Experiment groups were designed as follows. 1-Control, 2-Hypothyroidism; To induce hypothyroidism PTU was applied by intraperitoneal as 10 mg/kg/day for 2 weeks. 3-Hypothyroidism + Thyroxine; Previously animals were made with hypothyroidism by 1 week PTU application and then 1 week l-thyroxine was given by intraperitoneal as 1.5 mg/kg/day. 4-Hyperthyroidism; Rats were made with hyperthyroidism by 3 weeks l-thyroxine (0.3 mg/kg/day). 5-Hyperthyroidism + PTU; Animals were made hyperthyroisim by l-thyroxine as groups 4, then 1 week PTU was applied to treatment of hiperthyrodism. At the end of supplementation animals were sacrificed and blood samples were collected for FT3, FT4, adropin, asprosin, preptin analysis.
Results
FT3 ve FT4 levels were reduced significantly in hypothyroidism while increased in hyperthyroidism (p<0.001). Hipothyrodism led to reduces adropin, asprosin and preptin levels. And also hyperthyroidism reduced adropin and preptin levels (p<0.001).
Conclusions
The results of study show that experimental hypothyroidism and hyperthyroidism lead to significantly change to adropin, asprosin and preptin levels. However, correction of thyroid function caused to normals levels in asprosin and preptin.
Funding source: Selcuk University, Scientific Research Council
Award Identifier / Grant number: 19202002
Research funding: This study was supported by a grant by Selcuk University, Scientific Research Council (Grant number is 19202002).
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: All authors declare that there is no conflict of interest.
Informed consent: Not applicable.
Ethical approval: Ethical approval of the study was obtained from the ethics committee of the Selcuk University Experimental Medicine Research and Application Center (2019-8).
References
1. McAninch, EA, Bianco, AC. Thyroid hormone signaling in energy homeostasis and energy metabolism. Ann N Y Acad Sci 2014;1311:77–87. https://doi.org/10.1111/nyas.12374.Search in Google Scholar PubMed PubMed Central
2. Mullur, R, Liu, YY, Brent, GA. Thyroid hormone regulation of metabolism. Physiol Rev 2014;94:355–82. https://doi.org/10.1152/physrev.00030.2013.Search in Google Scholar PubMed PubMed Central
3. Sawicka, B, Bossowski, A, Szalecki, M, Wysoka, J, Koput, A, Zelazowska-Rutkowska, B, et al.. Relationship between metabolic parameters and thyroid hormones and the level of gastric peptides in children with autoimmune thyroid diseases. J Pediatr Endocrinol Metab 2010;23:345–54. https://doi.org/10.1515/jpem.2010.055.Search in Google Scholar PubMed
4. Martínez-Sánchez, N, Alvarez, CV, Fernø, J, Nogueiras, R, Diéguez, C, López, M. Hypothalamic effects of thyroid hormones on metabolism. Best practice & research. Best Pract Res Clin Endocrinol Metabol 2014;28:703–12. https://doi.org/10.1016/j.beem.2014.04.004.Search in Google Scholar PubMed
5. Little, AG. A review of the peripheral levels of regulation by thyroid hormone. J Comp Physiol B 2016;186:677–88. https://doi.org/10.1007/s00360-016-0984-2.Search in Google Scholar PubMed
6. Senese, R, de Lange, P, Petito, G, Moreno, M, Goglia, F, Lanni, A. 3,5-Diiodothyronine: a novel thyroid hormone metabolite and potent modulator of energy metabolism. Front Endocrinol 2018;25:427.10.3389/fendo.2018.00427Search in Google Scholar PubMed PubMed Central
7. Mierzwicka, A, Bolanowski, M. New peptides players in metabolic disorders. Postepy Hig Med Dosw;70:881–6. https://doi.org/10.5604/17322693.1216271.Search in Google Scholar PubMed
8. Ganesh Kumar, K, Zhang, J, Gao, S, Rossi, J, McGuinness, OP, Halem, HH, et al.. Adropin deficiency is associated with increased adiposity and insulin resistance. Obesity 2012;20:1394–402. https://doi.org/10.1038/oby.2012.31.Search in Google Scholar PubMed PubMed Central
9. Celik, E, Yilmaz, E, Celik, O, Ulas, M, Turkcuoglu, I, Karaer, A, et al.. Maternal and fetal adropin levels in gestational diabetes mellitus. J Perinat Med 2013;41:375–80. https://doi.org/10.1515/jpm-2012-0227.Search in Google Scholar PubMed
10. Chen, RM, Yuan, X, Ouyang, Q, Lin, XQ, Ai, ZZ, Zhang, Y, et al.. Adropin and glucagon-like peptide-2 are associated with glucose metabolism in obese children. World J Pediatr 2019;15:565–71. https://doi.org/10.1007/s12519-019-00296-6.Search in Google Scholar PubMed
11. Gao, S, Ghoshal, S, Zhang, L, Stevens, JR, McCommis, KS, Finck, BN, et al.. The peptide hormone adropin regulates signal transduction pathways controlling hepatic glucose metabolism in a mouse model of diet-induced obesity. J Biol Chem 2019;294:13366–77. https://doi.org/10.1074/jbc.ra119.008967.Search in Google Scholar
12. Wang, H, Wang, X, Cao, Y, Han, W, Guo, Y, Yang, G, et al.. Association of polymorphisms of preptin, irisin and adropin genes with susceptibility to coronary artery disease and hypertension. Medicine (Baltim) 2020;99: e19365. https://doi.org/10.1097/md.0000000000019365.Search in Google Scholar PubMed PubMed Central
13. Xiao, C, Li, W, Lu, T, Wang, J, Han, J. Preptin promotes proliferation and osteogenesis of MC3T3-E1 cells by upregulating β-catenin expression. IUBMB Life 2019;71:854–62. https://doi.org/10.1002/iub.2016.Search in Google Scholar PubMed
14. Mierzwicka, A, Kuliczkowska-Plaksej, J, Kolačkov, K, Bolanowski, M. Preptin in women with polycystic ovary syndrome. Gynecol Endocrinol 2018;34:470–5. https://doi.org/10.1080/09513590.2017.1409715.Search in Google Scholar PubMed
15. Mohammad Rahimi, GR, Bijeh, N, Rashidlamir, A. Effects of exercise training on serum preptin, undercarboxylated osteocalcin and high molecular weight adiponectin in adults with metabolic syndrome. Exp Physiol 2020;105:449–59. https://doi.org/10.1113/ep088036.Search in Google Scholar
16. Glück, M, Glück, J, Wiewióra, M, Rogala, B, Piecuch, J. Serum irisin, adropin, and preptin in obese patients 6 Months after bariatric surgery. Obes Surg 2019;29:3334–41. https://doi.org/10.1007/s11695-019-03998-y.Search in Google Scholar PubMed
17. Yuan, M, Li, W, Zhu, Y, Yu, B, Wu, J. Asprosin: a novel player in metabolic diseases. Front Endocrinol 2020;11:64. https://doi.org/10.3389/fendo.2020.00064.Search in Google Scholar PubMed PubMed Central
18. Hoffmann, JG, Xie, W, Chopra, AR. Energy regulation mechanism and therapeutic potential of asprosin. Diabetes 2020;69:559–66. https://doi.org/10.2337/dbi19-0009.Search in Google Scholar PubMed PubMed Central
19. Bhadel, P, Shrestha, S, Sapkota, B, Li, JY, Tao, H. Asprosin and type 2 diabetes mellitus: a novel potential therapeutic implication. J Biol Regul Homeost Agents 2020;34. https://doi.org/10.23812/19-244-E.Search in Google Scholar PubMed
20. Atici, E, Menevse, E, Baltaci, AK, Mogulkoc, R. Both experimental hypothyroidism and hyperthyroidism increase cardiac irisin levels in rats. Bratisl Lek Listy 2018;119:32–5. https://doi.org/10.4149/bll_2018_007.Search in Google Scholar
21. Atici, E, Mogulkoc, R, Baltaci, AK, Menevse, E. Both hypothyroidism and hyperthyroidism increase plasma irisin levels in rats. Horm Mol Biol Clin Invest 2017;33. j/hmbci.2018.33.issue-3/hmbci-2017-0054/hmbci-2017-0054.xml.10.1515/hmbci-2017-0054Search in Google Scholar PubMed
22. Atici, E, Mogulkoc, R, Baltaci, AK, Menevse, E. The effect of thyroid dysfunction on nesfatin-1 and adiponectin levels in rats. Horm Mol Biol Clin Invest 2017;32. j/hmbci.2017.32.issue-3/hmbci-2017-0033/hmbci-2017-0033.xml.10.1515/hmbci-2017-0033Search in Google Scholar PubMed
23. Kumar, KG, Trevaskis, JL, Lam, DD, Sutton, GM, Koza, RA, Chouljenko, VN, et al.. Identification of adropin as a secreted factor linking dietary macronutrient intake with energy homeostasis and lipid metabolism. Cell Metabol 2008;8:468–81. https://doi.org/10.1016/j.cmet.2008.10.011.Search in Google Scholar PubMed PubMed Central
24. Altamimi, TR, Gao, S, Karwi, QG, Fukushima, A, Rawat, S, Wagg, CS, et al.. Adropin regulates cardiac energy metabolism and improves cardiac function and efficiency. Metabolism 2019;98:37–48. https://doi.org/10.1016/j.metabol.2019.06.005.Search in Google Scholar PubMed
25. Li, S, Sun, J, Hu, W, Liu, Y, Lin, D, Duan, H, et al.. The association of serum and vitreous adropin concentrations with diabetic retinopathy. Ann Clin Biochem 2019;56:253–8. https://doi.org/10.1177/0004563218820359.Search in Google Scholar PubMed
26. Sato, K, Yamashita, T, Shirai, R, Shibata, K, Okano, T, Yamaguchi, M, et al.. Adropin contributes to anti-atherosclerosis by suppressing monocyte-endothelial cell adhesion and smooth muscle cell proliferation. Int J Mol Sci 2018;19:1293. https://doi.org/10.3390/ijms19051293.Search in Google Scholar PubMed PubMed Central
27. Oruc, Y, Celik, F, Ozgur, G, Beyazyildiz, E, Ugur, K, Yardim, M, et al.. Altered blood and aqueous humor levels of asprosın, 4-hydroxynonenal, and 8-hydroxy-deoxyguanosıne in patients with diabetes mellitus and cataract with and without diabetic retinopathy. Retina 2020. https://doi.org/10.1097/IAE.0000000000002776.Search in Google Scholar PubMed
28. Zhong, L, Long, Y, Wang, S, Lian, R, Deng, L, Ye, Z, et al.. Continuous elevation of plasma asprosin in pregnant women complicated with gestational diabetes mellitus: a nested case-control study. Placenta 2020;93:17–22. https://doi.org/10.1016/j.placenta.2020.02.004.Search in Google Scholar PubMed
29. El-Eshmawy, M, Abdel Aal, I. Relationships between preptin and osteocalcin in obese, overweight, and normal weight adults. Appl Physiol Nutr Metabol 2015;40:218–22. https://doi.org/10.1139/apnm-2014-0338.Search in Google Scholar PubMed
30. Alubaidi, TA, Mohammd, TU, Alkarawi, IN. Estimation of preptin in serum of thyroid dysfunction patients and its relationship with other parameters. Orient J Chem 2018:2114–24.10.13005/ojc/3404052Search in Google Scholar
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Articles in the same Issue
- Frontmatter
- Letter to the Editor
- Kerala model for combating COVID-19 pandemic
- Original Articles
- Evaluation of the potential anticancer activity of different vitamin D metabolites on colorectal and breast cancer cell lines
- Relationship between the levels of serum fibroblast growth factor 19 and metabolic factors in obese and normal weight subjects with and without type 2 diabetes mellitus: a case-control study
- Early-life stress altered pancreatic Krebs cycle-related enzyme activities in response to young adulthood physical and psychological stress in male rat offspring
- Effect of short-term and long-term traffic noise exposure on the thyroid gland in adult rats: a sexual dimorphic study
- The effect of thyroid dysfunction and treatment on adropin, asprosin and preptin levels in rats
- Comparison of the effects of zinc oxide and zinc oxide nanoparticles on the expression of hepcidin gene in rat liver
- Comparison between Iberet Folic® and Zincofer® in treatment of iron deficiency anaemia in pregnancy
- Mini Review
- COVID-19 and pregnancy: are they friends or enemies?
- Review Articles
- COVID-19: a review on SARS-CoV-2 origin, epidemiology, virology, clinical manifestations and complications with special emphasis on adverse outcome in Bhopal Gas Tragedy survivor
- COVID-19 and neurology perspective
- COVID-19 and nutritional deficiency: a review of existing knowledge
- COVID-19 and geriatric population: from pathophysiology to clinical perspectives
- COVID-19 and cardiovascular disease: a review of current knowledge
- How do different histologic components of mixed endometrial carcinomas affect prognosis? Does it really matter?
Articles in the same Issue
- Frontmatter
- Letter to the Editor
- Kerala model for combating COVID-19 pandemic
- Original Articles
- Evaluation of the potential anticancer activity of different vitamin D metabolites on colorectal and breast cancer cell lines
- Relationship between the levels of serum fibroblast growth factor 19 and metabolic factors in obese and normal weight subjects with and without type 2 diabetes mellitus: a case-control study
- Early-life stress altered pancreatic Krebs cycle-related enzyme activities in response to young adulthood physical and psychological stress in male rat offspring
- Effect of short-term and long-term traffic noise exposure on the thyroid gland in adult rats: a sexual dimorphic study
- The effect of thyroid dysfunction and treatment on adropin, asprosin and preptin levels in rats
- Comparison of the effects of zinc oxide and zinc oxide nanoparticles on the expression of hepcidin gene in rat liver
- Comparison between Iberet Folic® and Zincofer® in treatment of iron deficiency anaemia in pregnancy
- Mini Review
- COVID-19 and pregnancy: are they friends or enemies?
- Review Articles
- COVID-19: a review on SARS-CoV-2 origin, epidemiology, virology, clinical manifestations and complications with special emphasis on adverse outcome in Bhopal Gas Tragedy survivor
- COVID-19 and neurology perspective
- COVID-19 and nutritional deficiency: a review of existing knowledge
- COVID-19 and geriatric population: from pathophysiology to clinical perspectives
- COVID-19 and cardiovascular disease: a review of current knowledge
- How do different histologic components of mixed endometrial carcinomas affect prognosis? Does it really matter?
