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Detection and quantification of M-proteinemia: comparison of various methods for serum protein electrophoresis

  • Andries J. Bakker EMAIL logo , Coby Elderman-van der Werf und Tjallie van Abbema
Veröffentlicht/Copyright: 28. September 2011
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Clinical Chemistry and Laboratory Medicine
Aus der Zeitschrift Clinical Chemistry and Laboratory Medicine Band 50 Heft 1

Abstract

Background: Detection and quantification of monoclonal proteins is hampered when the monoclonal peak coincides with one of the regular bands in serum proteinelectrophoresis. The objective of this study was to evaluate four procedures for serum proteinelectrophoresis with respect to detection and quantification of monoclonal proteins.

Methods: For 466 patient samples with a monoclonal protein, three variants of agarose gel electrophoresis (5-band, split-β and high resolution) and one variant of capillary electrophoresis were compared with the results of the routinely used agarose gel electrophoresis followed by immunofixation analysis using specific or pentavalent antisera.

Results: In total, 310 patient samples were analyzed by the four methods, consisting of 295 samples with a monoclonal protein, seven with oligoclonal bands and eight without any bands. Suspicion of a monoclonal protein was raised in 295/256/256/232/265 of the samples using the reference/5-band/split-β/high resolution/capillary procedure. In 152/147/135/142/126 of the samples the concentration of monoclonal protein was >1.0 g/L and in 51/33/53/33/67 of the cases, the monoclonal protein was not separated from one of the normal protein zones.

Conclusions: In high resolution agarose gel electrophoresis, monoclonal bands of low concentration often remain undetected. In split-β agarose gel electrophoresis as well as capillary electrophoresis monoclonal bands more often were not separated from the regular protein bands.

Keywords: agarose gel electrophoresis (AGE) variants; capillary zone electrophoresis (CZE); monoclonal gammo­pathy; M-proteinemia; serum protein electrophoresis (SPE)
Corresponding author: Andries J. Bakker, PhD, Department of Clinical Chemistry, Klinisch Chemisch Laboratorium, P.O. Box 850, 8901 BR Leeuwarden, The Netherlands Phone: +31-582888444, Fax: +31-582882227
Received: 2011-8-23
Accepted: 2011-9-7
Published Online: 2011-09-28
Published in Print: 2012-01-01

©2011 by Walter de Gruyter Berlin Boston

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https://doi.org/10.1515/cclm.2011.723
Schlagwörter für diesen Artikel
agarose gel electrophoresis (AGE) variants; capillary zone electrophoresis (CZE); monoclonal gammo­pathy; M-proteinemia; serum protein electrophoresis (SPE)

Artikel in diesem Heft

  1. Editorial
  2. Targeted therapy for HER-2: personalized medicine for her, too
  3. Review
  4. Circulating levels of HER-2/neu oncoprotein in breast cancer
  5. Mini Review
  6. The human epidermal growth factor receptor 2 (HER2)
  7. Opinion Paper
  8. Proposed criteria for sorting examined properties in clinical laboratory reports
  9. Guidelines and Recommendations
  10. Properties and units in the clinical laboratory sciences, Part XXIII. The NPU terminology, principles and implementation –a user’s guide (Technical Report 2011) (IFCC–IUPAC)
  11. Genetics and Molecular Diagnostics
  12. Establishing high resolution melting analysis: method validation and evaluation for c-RET proto-oncogene mutation screening
  13. Decreased mRNA expression of CCL5 [RANTES] in Alzheimer's disease blood samples
  14. General Clinical Chemistry and Laboratory Medicine
  15. A risk-analysis approach to the evaluation of analytical quality
  16. C-statistics versus logistic regression for assessing the performance of qualitative diagnostic tests
  17. Detection and quantification of M-proteinemia: comparison of various methods for serum protein electrophoresis
  18. External quality assessment of point-of-care International Normalized Ratio (INR) testing in Europe
  19. Comparison of two point-of-care testing (POCT) devices for fetal lactate during labor
  20. Simultaneous quantification of 19 drugs/metabolites in urine important for pain management by liquid chromatography-tandem mass spectrometry
  21. Enzyme-linked immunoassay for plasma-free metanephrines in the biochemical diagnosis of phaeochromocytoma in adults is not ideal
  22. The old and new tests for celiac disease: which is the best test combination to diagnose celiac disease in pediatric patients?
  23. Improving diagnosis of adult-type hypolactasia in patients with abdominal complaints
  24. Synovial and serum levels of uncarboxylated matrix Gla-protein (ucMGP) in patients with arthritis
  25. The diagnostic performance of allergen-molecules in comparison to allergen-extracts
  26. Analytical performances of cystatin C turbidimetric assay: which impact on accuracy of glomerular filtration rate estimation in renal transplantation?
  27. Evaluation of Aution Max AX-4030 and 9UB Uriflet, 10PA Aution Sticks urine dipsticks in the automated urine test strip analysis
  28. Reference Values and Biological Variations
  29. Diurnal variability of total calcium during normal sleep and after an acute shift of sleep
  30. Infections Diseases
  31. Comparison of two immunoassays for determining hepatitis B virus serum markers
  32. Cardiovascular Diseases
  33. Ultra-sensitive troponin I levels to exclude acute myocardial infarction from myocardial injury
  34. Diabetes
  35. Extracellular matrix-associated (GAGs, CTGF), angiogenic (VEGF) and inflammatory factors (MCP-1, CD40, IFN-γ) in type 1 diabetes mellitus nephropathy
  36. Letters to the Editor
  37. Serum HER-2/ECD analysis in monitoring breast cancer patients
  38. No evidence for the involvement of the lipoxin A4 receptor (FPR2/ALX) gene in the susceptibility to coronary artery disease
  39. Does the presence of 3-epi-25OHD3 affect the routine measurement of vitamin D using liquid chromatography tandem mass spectrometry?
  40. Validity of the measurement of capillary cholesterol in the diagnosis of defined hypercholesterolemia
  41. Prelims
  42. Prelims
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