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Simultaneous quantification of 19 drugs/metabolites in urine important for pain management by liquid chromatography-tandem mass spectrometry

  • Chao Yuan , Courtney Heideloff , Marta Kozak and Sihe Wang EMAIL logo
Published/Copyright: October 8, 2011
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Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 50 Issue 1

Abstract

Background: Monitoring pain management drugs and frequently abused drugs is important for physicians to assess patient compliance. Liquid chromatography-tandem mass spectrometry offers high specificity needed for this purpose. In this report, a novel liquid chromatography-tandem mass spectrometry method for simultaneously monitoring 19 drugs/metabolites in urine was developed and validated.

Methods: Sample preparation included hydrolysis, dilution and turbulent flow online extraction. Analysis was achieved by reverse phase liquid chromatography and triple-quadruple tandem mass spectrometry. Two fragment ions, one for quantification and the other for assuring identification, were monitored for each analyte.

Results: No matrix effect or interference was observed. Lower limits of quantification ranged from 5 to 25 ng/mL. Within the linear range, analytical recovery was between 85.8% and 119.4%. Intra-assay and total coefficient of variations were between 0.2% and 12.7%. This method was compared with mass spectrometry methods offered by two other laboratories using 82 patient samples and 60 spiked urine samples showing 60%–100% agreement. The current method identified more positive samples for all analytes except THCA. The discrepancy in detection rates was primarily due to the different cut-offs used by the other laboratories.

Conclusions: A sensitive and specific liquid chromatography-tandem mass spectrometry method was developed for measuring 19 drugs/metabolites in urine important for pain management clinics.

Keywords: drugs of abuse; liquid chromatography-tandem mass spectrometry; pain management; quantification; turbulent flow
Corresponding author: Sihe Wang, Department of Clinical Pathology, Mail Code L11, 9500 Euclid Ave, Cleveland, OH 44195, USA Phone: +1 216-445-2634, Fax: +1 216-444-4414
Received: 2011-7-22
Accepted: 2011-9-13
Published Online: 2011-10-08
Published in Print: 2012-01-01

©2011 by Walter de Gruyter Berlin Boston

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https://doi.org/10.1515/cclm.2011.739
Keywords for this article
drugs of abuse; liquid chromatography-tandem mass spectrometry; pain management; quantification; turbulent flow

Articles in the same Issue

  1. Editorial
  2. Targeted therapy for HER-2: personalized medicine for her, too
  3. Review
  4. Circulating levels of HER-2/neu oncoprotein in breast cancer
  5. Mini Review
  6. The human epidermal growth factor receptor 2 (HER2)
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  9. Guidelines and Recommendations
  10. Properties and units in the clinical laboratory sciences, Part XXIII. The NPU terminology, principles and implementation –a user’s guide (Technical Report 2011) (IFCC–IUPAC)
  11. Genetics and Molecular Diagnostics
  12. Establishing high resolution melting analysis: method validation and evaluation for c-RET proto-oncogene mutation screening
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  14. General Clinical Chemistry and Laboratory Medicine
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  22. The old and new tests for celiac disease: which is the best test combination to diagnose celiac disease in pediatric patients?
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  30. Infections Diseases
  31. Comparison of two immunoassays for determining hepatitis B virus serum markers
  32. Cardiovascular Diseases
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