Home Synovial and serum levels of uncarboxylated matrix Gla-protein (ucMGP) in patients with arthritis
Article
Licensed
Unlicensed Requires Authentication

Synovial and serum levels of uncarboxylated matrix Gla-protein (ucMGP) in patients with arthritis

  • Ciprian N. Silaghi , Dana Fodor , Victor Cristea and Alexandra M. Crăciun EMAIL logo
Published/Copyright: September 17, 2011
Become an author with De Gruyter Brill
Submit Manuscript Author Information
Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 50 Issue 1

Abstract

Background: Matrix Gla-protein (MGP) is a calcification inhibitor produced by cartilage and vessel walls. Arthritis is defined by inflammation, cartilage and bone destruction/formation and articular calcifications. Our objectives were to evaluate ucMGP levels in synovial fluid (SF) in arthritis patients and to investigate the relationship between local and circulating ucMGP and their association with age and inflammation.

Methods: Arthritis patients (n=26) with knee joint effusion and controls (n=30) underwent an ultrasonographic knee examination for articular calcifications assessment. Patients were divided into inflammatory and non-inflammatory groups. ucMGP levels were determined in serum and SF using a competitive ELISA assay.

Results: Within the arthritis patients, the inflammatory group had the lowest ucMGP serum levels, and the highest levels of synovial ucMGP. Furthermore, the inflammatory group had significantly (p<0.05) higher RucMGP (synovial ucMGP/serum ucMGP*100) than the non-inflammatory group [36 (17–69) vs. 24 (5–55)], and this parameter positively correlated (r=0.4; p<0.05) with the erythrocyte sedimentation rate (ESR). No correlation was found between age and local or circulating ucMGP in patients and controls.

Conclusions: The ucMGP assay can be used for determination of MGP in SF, and combined assessment of ucMGP in serum and SF could potentially serve as a joint inflammatory marker in arthritis patients.

Keywords: arthritis; articular calcifications; inflammation; matrix Gla-protein; synovial fluid
Corresponding author: Alexandra M. Crăciun, MD, PhD, Department of Medical Biochemistry, University of Medicine and Pharmacy, “Iuliu Haţieganu”, 6 Pasteur Street, 400349, Cluj-Napoca, Romania Phone: +40-722-348226, Fax: +40-264-595965
Received: 2011-6-4
Accepted: 2011-8-28
Published Online: 2011-09-17
Published in Print: 2012-01-01

©2011 by Walter de Gruyter Berlin Boston

Articles in the same Issue

  1. Editorial
  2. Targeted therapy for HER-2: personalized medicine for her, too
  3. Review
  4. Circulating levels of HER-2/neu oncoprotein in breast cancer
  5. Mini Review
  6. The human epidermal growth factor receptor 2 (HER2)
  7. Opinion Paper
  8. Proposed criteria for sorting examined properties in clinical laboratory reports
  9. Guidelines and Recommendations
  10. Properties and units in the clinical laboratory sciences, Part XXIII. The NPU terminology, principles and implementation –a user’s guide (Technical Report 2011) (IFCC–IUPAC)
  11. Genetics and Molecular Diagnostics
  12. Establishing high resolution melting analysis: method validation and evaluation for c-RET proto-oncogene mutation screening
  13. Decreased mRNA expression of CCL5 [RANTES] in Alzheimer's disease blood samples
  14. General Clinical Chemistry and Laboratory Medicine
  15. A risk-analysis approach to the evaluation of analytical quality
  16. C-statistics versus logistic regression for assessing the performance of qualitative diagnostic tests
  17. Detection and quantification of M-proteinemia: comparison of various methods for serum protein electrophoresis
  18. External quality assessment of point-of-care International Normalized Ratio (INR) testing in Europe
  19. Comparison of two point-of-care testing (POCT) devices for fetal lactate during labor
  20. Simultaneous quantification of 19 drugs/metabolites in urine important for pain management by liquid chromatography-tandem mass spectrometry
  21. Enzyme-linked immunoassay for plasma-free metanephrines in the biochemical diagnosis of phaeochromocytoma in adults is not ideal
  22. The old and new tests for celiac disease: which is the best test combination to diagnose celiac disease in pediatric patients?
  23. Improving diagnosis of adult-type hypolactasia in patients with abdominal complaints
  24. Synovial and serum levels of uncarboxylated matrix Gla-protein (ucMGP) in patients with arthritis
  25. The diagnostic performance of allergen-molecules in comparison to allergen-extracts
  26. Analytical performances of cystatin C turbidimetric assay: which impact on accuracy of glomerular filtration rate estimation in renal transplantation?
  27. Evaluation of Aution Max AX-4030 and 9UB Uriflet, 10PA Aution Sticks urine dipsticks in the automated urine test strip analysis
  28. Reference Values and Biological Variations
  29. Diurnal variability of total calcium during normal sleep and after an acute shift of sleep
  30. Infections Diseases
  31. Comparison of two immunoassays for determining hepatitis B virus serum markers
  32. Cardiovascular Diseases
  33. Ultra-sensitive troponin I levels to exclude acute myocardial infarction from myocardial injury
  34. Diabetes
  35. Extracellular matrix-associated (GAGs, CTGF), angiogenic (VEGF) and inflammatory factors (MCP-1, CD40, IFN-γ) in type 1 diabetes mellitus nephropathy
  36. Letters to the Editor
  37. Serum HER-2/ECD analysis in monitoring breast cancer patients
  38. No evidence for the involvement of the lipoxin A4 receptor (FPR2/ALX) gene in the susceptibility to coronary artery disease
  39. Does the presence of 3-epi-25OHD3 affect the routine measurement of vitamin D using liquid chromatography tandem mass spectrometry?
  40. Validity of the measurement of capillary cholesterol in the diagnosis of defined hypercholesterolemia
  41. Prelims
  42. Prelims
https://doi.org/10.1515/cclm.2011.713
Keywords for this article
arthritis; articular calcifications; inflammation; matrix Gla-protein; synovial fluid

Articles in the same Issue

  1. Editorial
  2. Targeted therapy for HER-2: personalized medicine for her, too
  3. Review
  4. Circulating levels of HER-2/neu oncoprotein in breast cancer
  5. Mini Review
  6. The human epidermal growth factor receptor 2 (HER2)
  7. Opinion Paper
  8. Proposed criteria for sorting examined properties in clinical laboratory reports
  9. Guidelines and Recommendations
  10. Properties and units in the clinical laboratory sciences, Part XXIII. The NPU terminology, principles and implementation –a user’s guide (Technical Report 2011) (IFCC–IUPAC)
  11. Genetics and Molecular Diagnostics
  12. Establishing high resolution melting analysis: method validation and evaluation for c-RET proto-oncogene mutation screening
  13. Decreased mRNA expression of CCL5 [RANTES] in Alzheimer's disease blood samples
  14. General Clinical Chemistry and Laboratory Medicine
  15. A risk-analysis approach to the evaluation of analytical quality
  16. C-statistics versus logistic regression for assessing the performance of qualitative diagnostic tests
  17. Detection and quantification of M-proteinemia: comparison of various methods for serum protein electrophoresis
  18. External quality assessment of point-of-care International Normalized Ratio (INR) testing in Europe
  19. Comparison of two point-of-care testing (POCT) devices for fetal lactate during labor
  20. Simultaneous quantification of 19 drugs/metabolites in urine important for pain management by liquid chromatography-tandem mass spectrometry
  21. Enzyme-linked immunoassay for plasma-free metanephrines in the biochemical diagnosis of phaeochromocytoma in adults is not ideal
  22. The old and new tests for celiac disease: which is the best test combination to diagnose celiac disease in pediatric patients?
  23. Improving diagnosis of adult-type hypolactasia in patients with abdominal complaints
  24. Synovial and serum levels of uncarboxylated matrix Gla-protein (ucMGP) in patients with arthritis
  25. The diagnostic performance of allergen-molecules in comparison to allergen-extracts
  26. Analytical performances of cystatin C turbidimetric assay: which impact on accuracy of glomerular filtration rate estimation in renal transplantation?
  27. Evaluation of Aution Max AX-4030 and 9UB Uriflet, 10PA Aution Sticks urine dipsticks in the automated urine test strip analysis
  28. Reference Values and Biological Variations
  29. Diurnal variability of total calcium during normal sleep and after an acute shift of sleep
  30. Infections Diseases
  31. Comparison of two immunoassays for determining hepatitis B virus serum markers
  32. Cardiovascular Diseases
  33. Ultra-sensitive troponin I levels to exclude acute myocardial infarction from myocardial injury
  34. Diabetes
  35. Extracellular matrix-associated (GAGs, CTGF), angiogenic (VEGF) and inflammatory factors (MCP-1, CD40, IFN-γ) in type 1 diabetes mellitus nephropathy
  36. Letters to the Editor
  37. Serum HER-2/ECD analysis in monitoring breast cancer patients
  38. No evidence for the involvement of the lipoxin A4 receptor (FPR2/ALX) gene in the susceptibility to coronary artery disease
  39. Does the presence of 3-epi-25OHD3 affect the routine measurement of vitamin D using liquid chromatography tandem mass spectrometry?
  40. Validity of the measurement of capillary cholesterol in the diagnosis of defined hypercholesterolemia
  41. Prelims
  42. Prelims
Sign up now to receive a 20% welcome discount
Institutional Access
How does access work?
Have an idea on how to improve our website?
Please write us.
© 2025 De Gruyter Brill
Downloaded on 20.11.2025 from https://www.degruyterbrill.com/document/doi/10.1515/cclm.2011.713/html
Scroll to top button