Comparative Nuclear Magnetic Resonance Studies of Water Permeability of Red Blood Cells from Maternal Venous Blood and Newborn Umbilical Cord Blood
-
Gheorghe Benga
Abstract
Comparative morphological and nuclear magnetic resonance (NMR) measurements of the diffusional permeability (Pd) were performed on red blood cells (RBCs) from maternal venous blood and fetal RBCs, isolated from cord blood taken at delivery. Fetal RBC had a diameter of 8.79±0.03 μm (mean±standard deviation, SD), a volume of 103 μm3 and a surface area of 157 μm2. We report here the first comparative measurements of Pd of maternal and fetal RBCs by using a Mn2+-doping NMR technique. The values of Pd were, in the case of maternal RBC, 3.7 × 10−3 cm/s at 15 °C, 4.1 × 10−3 cm/s at 20 °C, 4.9 × 10−3 cm/s at 25 °C, 5.2 × 10−3 cm/s at 30 °C and 7.2 × 10−3 cm/s at 37 °C. For fetal RBC all corresponding Pd values were almost half, namely 2.0 × 10−3 cm/s at 15 °C, 2.3 × 10−3 cm/s at 20 °C, 2.8 × 10−3 cm/s at 25 °C, 3.4 × 10−3 cm/s at 30 °C and 4.4 × 10−3 cm/s at 37 °C. The decreased Pd values of fetal RBCs were probably due to lower channel-mediated water permeability compared with adult RBCs. The values of the activation energy for water permeability (Ea,d) were significantly higher for fetal RBCs (27.6±5.0 kJ/mol) than for adult RBCs (22.8±2.7 kJ/mol). A positive correlation between the Pd values of the two kinds of RBCs was found. This points to the genetic basis for the determination of RBC water permeability.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
Artikel in diesem Heft
- The Impact of Cardiac Natriuretic Peptide Determination on the Diagnosis and Management of Heart Failure
- Models for Combining Random and Systematic Errors. Assumptions and Consequences for different Models
- Nucleosomes in Serum as a Marker for Cell Death
- Comparative Nuclear Magnetic Resonance Studies of Water Permeability of Red Blood Cells from Maternal Venous Blood and Newborn Umbilical Cord Blood
- Optimization of Single-Stranded Conformation Polymorphism (SSCP) Analysis for Screening for the Estrogen Receptor-α Gene Polymorphism P325P
- Serum Total Renin after Tubal Sterilization
- Oxidative Stress and Male IGF-1, Gonadotropin and Related Hormones in Diabetic Patients
- New PCR-Based Method for the Sp1 Site Polymorphism in the COL1A1 Gene
- Comparability of Serum and Plasma Concentrations of Haemostasis Activation Markers
- Importance of Validation of Immunoassays for Intact Proinsulin
- Plant Sterol-Enriched Margarine Lowers Plasma LDL in Hyperlipidemic Subjects with low Cholesterol Intake: Effect of Fibrate Treatment
- Menopause, Coronary Artery Disease and Antioxidants
- Arterial Tissue of Arsenic, Selenium and Iron in Blackfoot Disease Patients
- Evaluation of Aution Max AX-4280 Automated Urine Test-Strip Analyser
- Evaluation of Automated Enzyme Immunoassays for the Detection of Antibodies to Extractable Nuclear Antigens
- Metrological Requirements for Clinical Laboratories
Artikel in diesem Heft
- The Impact of Cardiac Natriuretic Peptide Determination on the Diagnosis and Management of Heart Failure
- Models for Combining Random and Systematic Errors. Assumptions and Consequences for different Models
- Nucleosomes in Serum as a Marker for Cell Death
- Comparative Nuclear Magnetic Resonance Studies of Water Permeability of Red Blood Cells from Maternal Venous Blood and Newborn Umbilical Cord Blood
- Optimization of Single-Stranded Conformation Polymorphism (SSCP) Analysis for Screening for the Estrogen Receptor-α Gene Polymorphism P325P
- Serum Total Renin after Tubal Sterilization
- Oxidative Stress and Male IGF-1, Gonadotropin and Related Hormones in Diabetic Patients
- New PCR-Based Method for the Sp1 Site Polymorphism in the COL1A1 Gene
- Comparability of Serum and Plasma Concentrations of Haemostasis Activation Markers
- Importance of Validation of Immunoassays for Intact Proinsulin
- Plant Sterol-Enriched Margarine Lowers Plasma LDL in Hyperlipidemic Subjects with low Cholesterol Intake: Effect of Fibrate Treatment
- Menopause, Coronary Artery Disease and Antioxidants
- Arterial Tissue of Arsenic, Selenium and Iron in Blackfoot Disease Patients
- Evaluation of Aution Max AX-4280 Automated Urine Test-Strip Analyser
- Evaluation of Automated Enzyme Immunoassays for the Detection of Antibodies to Extractable Nuclear Antigens
- Metrological Requirements for Clinical Laboratories
