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Cassava is a strategic crop, especially for developing countries. However, the presence of cyanogenic compounds in cassava products limits the proper nutrients utilization. Due to the poor availability of structure discovery and elucidation in the Protein Data Bank is limiting the full understanding of the enzyme, how to inhibit it and applications in different fields. There is a need to solve the three-dimensional structure (3-D) of linamarase from cassava. The structural elucidation will allow the development of a competitive inhibitor and various industrial applications of the enzyme. The goal of this review is to summarize and present the available 3-D modeling structure of linamarase enzyme using different computational strategies. This approach could help in determining the structure of linamarase and later guide the structure elucidation in silico and experimentally.

Ion exchange is one of the promising methods for downstream processing in biotechnology. Its advantages are based on selectivity and therefore obtaining of products with reasonable concentration and purity, mild conditions, simple operation and saving of time and energy for product separation. Additional advantage is the possible in situ extraction of ionogenic products from the fermentation broth, including removal of potential inhibitors during the fermentation process. In the case of biotechnology, ion exchange could be considered in two separate ways: ion-exchange solvent extraction and traditional liquid/solid ion exchange by ion-exchange resins. Both approaches have been studied. In this paper, this approach is shown on two important case studies: L(+)-lactic acid and L-lysine recovery from fermentation processes.

This paper provides a brief overview of the formation, organization, and mission of the School of Green Chemistry and Engineering (SGCE) at the University of Toledo, and a description of SGCE efforts to introduce principles of green chemistry and green engineering into the undergraduate and graduate curriculum. This includes development of selected new courses and new academic programs such as a Minor in Green Chemistry and Engineering, a Professional Science Master’s Degree in Green Chemistry and Engineering, and an online Master in Education and Science in Chemistry degree aimed at College Credit Plus credentialing of high school chemistry teachers in Ohio.

The purpose of downstream processing in a fermentation technology is the isolation, purification and concentration of the final product. Membrane processes are generally used in these steps. In this paper, the application possibilities of polymer membranes in pressure-driven membrane techniques (microfiltration, ultrafiltration, nanofiltration), pervaporation, dialysis and electrodialysis are presented.

This article introduces the general characteristics of the diarylethene class of photochromic dye and the structural features that make photochromism possible. It touches on the methodologies employed to synthesize these compounds as well as the influences that typical substitution patterns exert on photocoloration. A demonstration is then given of the great diversity pertaining to the potential applications in which researchers are seeking to exploit them as functional colorants.