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Mechanical testing of single yeast cells in liquid environment: Effect of the extracellular osmotic conditions on the failure behavior

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Published/Copyright: June 11, 2013
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International Journal of Materials Research
From the journal International Journal of Materials Research Volume 100 Issue 7

Abstract

In industrial biotechnology microbial cells are utilized to produce diverse products (e. g., enzymes, food and animal feed additives, pharmaceutical agents). Whilst relationships on genetic and molecular levels are reasonably understood there are still numerous unsolved questions in matters of the mechanical properties of those cells. Recently, a compression test in liquid environment using a nanoindentation device was established to address the cell mechanical behavior of Saccharomyces cerevisiae (baker's yeast) cells. At higher deformations compressive stressing yielded failure of the cell wall. In the present work, the influence of the extracellular osmotic conditions on the failure characteristics (bursting force, bursting energy, and relative deformation at bursting) was studied. Moreover, a simple mechanical model was applied to characterize the mechanical properties of the cell wall.

Keywords: Nanoindentation; Compression testing; Biomaterials; Baker's yeast; Osmotic pressure
* Correspondence address, Judith Arfsten, Institute for Particle Technology, Volkmaroder Str. 5, D-38104 Braunschweig, Germany, Tel.: +49 531 3 91 70 99, Fax: +49 531 3 91 81 46, E-mail:

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Received: 2008-10-20
Accepted: 2009-2-1
Published Online: 2013-06-11
Published in Print: 2009-07-01

© 2009, Carl Hanser Verlag, München

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https://doi.org/10.3139/146.110141
Keywords for this article
Nanoindentation; Compression testing; Biomaterials; Baker's yeast; Osmotic pressure

Articles in the same Issue

  1. Contents
  2. Contents
  3. Feature
  4. Intermolecular slip mechanism in tropocollagen nanofibrils
  5. Basic
  6. Identification of model parameters from elastic/elasto-plastic spherical indentation
  7. Penetration of a pyramid indenter into a multilayer coating
  8. Nanoindentation of pseudoelastic NiTi shape memory alloys: Thermomechanical and microstructural aspects
  9. Analysis of nanoindentation curves in the case of bulk amorphous polymers
  10. Effect of indenter shapes on inverse materials characterization based on the dual indenters method
  11. Applied
  12. Creep properties from indentation tests by analytical and numerical techniques
  13. Analysis of nanoindentation and nanoscratch experiments of thin amorphous carbon coatings and multilayers: friction, wear and elastic – plastic deformation
  14. Mutual consistency of hardness testing at micro- and nanometer scales
  15. Friction and adhesion of carbon nanotube brushes
  16. Mechanical testing of single yeast cells in liquid environment: Effect of the extracellular osmotic conditions on the failure behavior
  17. UFG and nanocrystalline microstructures produced by hydrostatic extrusion of multifilament wires
  18. In-situ high temperature microstructural analysis during tempering of 42CrMo4 using transmission electron microscopy
  19. Mixing enthalpies in Ag–Ca, Ag–Eu and Ag–Yb liquid alloys
  20. Dilatometry revealing Si precipitation in Al–Si-alloys
  21. Notifications
  22. DGM News
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