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Stimuli-responsive polymer layers for advanced cell culture technologies

  • Brigitte Voit , Dirk Schmaljohann , Stefan Gramm , Mirko Nitschke und Carsten Werner
Veröffentlicht/Copyright: 23. Mai 2013
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International Journal of Materials Research
Aus der Zeitschrift International Journal of Materials Research Band 98 Heft 7

Abstract

A series of graft copolymers consisting of poly(N-isopropylacrylamide) as a thermoresponsive component in the polymer backbone and poly(ethyleneglycol) side chains were immobilized as thin films on various substrates via low-pressure plasma treatment. The surface-immobilized hydrogels exhibit a transition from partially collapsed to completely swollen, which is in the range of 32 – 35 °C and corresponds to the lower critical solution temperature of the soluble polymers. Cell culture and detachment experiments were performed with mouse fibroblasts and human corneal endothelial cells under standard conditions. The hydrogel-coated supports were found to permit adhesion, spreading and proliferation of the cells and allowed for fast and effective temperature-dependent detachment of intact cell sheets of both cell types. Thus, these coatings offer an efficient method for growth and gentle harvesting of functional cellular assemblies for use in tissue engineering strategies.

Keywords: Responsive polymers; Functional polymers; Thin films; Bioactive surfaces; Cell cultures
* Correspondence address, Prof. Dr. Brigitte Voit Leibniz-Institut für Polymerforschung Dresden e.V. Max-Bergmann-Zentrum für Biomaterialien Hohe Straße 6, D-01069 Dresden, Germany Tel.: +49 351 465 85 90 Fax: +49 351 465 89 65 E-mail:

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Received: 2007-2-23
Accepted: 2007-5-13
Published Online: 2013-05-23
Published in Print: 2007-07-01

© 2007, Carl Hanser Verlag, München

Artikel in diesem Heft

  1. Contents
  2. Contents
  3. Editorial
  4. Editorial
  5. Basic
  6. Diffusional decomposition of supersaturated solid solutions at grain boundaries
  7. Characterization of oriented protein-ceramic and protein-polymer-composites for cartilage tissue engineering using synchrotron μ-CT
  8. Effect of ageing on optical and thermal properties of YBa2Cu3O7–δ
  9. Calorimetric study and thermodynamic assessment of the SrO–Ga2O3 system
  10. Re-determination of transition temperatures in the Fe–Al system by differential thermal analysis
  11. Applied
  12. Novel corrosion protective coatings for aluminium alloys and steels based on oxidic nanoparticles
  13. Novel biomimetically based ice-nucleating coatings
  14. Biomimetically inspired hybrid materials based on silicified collagen
  15. Magnesium sponges as a bioabsorbable material – attributes and challenges
  16. Morphology of bony tissues and implants uncovered by high-resolution tomographic imaging
  17. Review
  18. The effects of metal implants on inflammatory and healing processes
  19. Formation of nano hydroxyapatite – a straightforward way to bioactivate bone implant surfaces
  20. Drug-eluting stent technologies for vascular regeneration
  21. Fiber structures for the regenerative medicine
  22. Stimuli-responsive polymer layers for advanced cell culture technologies
  23. Notifications
  24. Robert W. Cahn: 1924–2007
  25. DGM News
https://doi.org/10.3139/146.101517
Schlagwörter für diesen Artikel
Responsive polymers; Functional polymers; Thin films; Bioactive surfaces; Cell cultures

Artikel in diesem Heft

  1. Contents
  2. Contents
  3. Editorial
  4. Editorial
  5. Basic
  6. Diffusional decomposition of supersaturated solid solutions at grain boundaries
  7. Characterization of oriented protein-ceramic and protein-polymer-composites for cartilage tissue engineering using synchrotron μ-CT
  8. Effect of ageing on optical and thermal properties of YBa2Cu3O7–δ
  9. Calorimetric study and thermodynamic assessment of the SrO–Ga2O3 system
  10. Re-determination of transition temperatures in the Fe–Al system by differential thermal analysis
  11. Applied
  12. Novel corrosion protective coatings for aluminium alloys and steels based on oxidic nanoparticles
  13. Novel biomimetically based ice-nucleating coatings
  14. Biomimetically inspired hybrid materials based on silicified collagen
  15. Magnesium sponges as a bioabsorbable material – attributes and challenges
  16. Morphology of bony tissues and implants uncovered by high-resolution tomographic imaging
  17. Review
  18. The effects of metal implants on inflammatory and healing processes
  19. Formation of nano hydroxyapatite – a straightforward way to bioactivate bone implant surfaces
  20. Drug-eluting stent technologies for vascular regeneration
  21. Fiber structures for the regenerative medicine
  22. Stimuli-responsive polymer layers for advanced cell culture technologies
  23. Notifications
  24. Robert W. Cahn: 1924–2007
  25. DGM News
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