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Characterization of oriented protein-ceramic and protein-polymer-composites for cartilage tissue engineering using synchrotron μ-CT

  • Rolf Zehbe , Astrid Haibel , Christoph Brochhausen , Ulrich Gross , C. James Kirkpatrick and Helmut Schubert
Published/Copyright: May 23, 2013
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International Journal of Materials Research
From the journal International Journal of Materials Research Volume 98 Issue 7

Abstract

In this paper we report on the synthesis of three different gelatine based scaffold materials for the reconstruction of articular cartilage defects. The first scaffold design is based on an unmodified, oriented gelatine network, while the second design further comprises an attached inorganic hydroxyapatite layer and the third design includes poly(l-lactide) microspheres as a model material for future drug-release applications.

All three scaffold designs were characterized and imaged using synchrotron μ-CT, obtaining a complete volumetric reconstruction of a previously defined sample region.

Furthermore, two unmodified scaffolds were cultivated for one week with porcine chondrocytes. Afterwards the attached cells were labelled using a combination of Au-lysine and silver enhancer. In synchrotron μ-CT analysis we were thus able to map the cell distribution due to the difference in X-ray absorption of the labelled cells and the non labelled scaffolds in a volume of several millimetres.

Keywords: Tissue engineering; Joint cartilage; Synchrotron μ-CT imaging; Gelatine scaffold; Porcine chondrocytes
* Correspondence address, Mr. Rolf Zehbe Englische Straße 20, D-10587 Berlin, Germany Tel.: +49 30 31 42 40 72 Fax: +49 30 31 42 40 72 E-mail:

References

[1] V.C.Mow, W.M.Lai: J. Biomech.7 (1976) 449.10.1016/0021-9290(74)90007-4Search in Google Scholar

[2] J.H.C.Wang, F.Jia, T.W.Gilbert, S.L.Y.Woo: J. Biomech.36 (2003) 97.10.1016/S0021-9290(02)00233-6Search in Google Scholar

[3] A.G.Mikos, J.S.Temenoff: EJB Electronic Journal of Biotechnology3 (2000) 114.Search in Google Scholar

[4] R.Zehbe, U.Gross, H.Schubert: Key Eng. Mat.254–256 (2004) 1083.Search in Google Scholar

[5] N.Dagalakis, J.Flink, P.Stasikelis, J.Burke, I.V.Yannas: J. Biomed. Mater. Res.14 (1980) 511.10.1002/jbm.820140417Search in Google Scholar PubMed

[6] L.A.Feldkamp, S.A.Goldstein, A.M.Parfitt, G.Jesion, M.Kleerekope: J. Bone Min. Res.4 (1989) 11.Search in Google Scholar

[7] S.T.Ho, D.W.Hutmacher: Biomaterials27 (2006) 1362.10.1016/j.biomaterials.2005.08.035Search in Google Scholar PubMed

[8] A.L.Darling, W.Sun: J. Biomed. Mater. Res. B70 (2004) 311.10.1002/jbm.b.30050Search in Google Scholar PubMed

[9] S.Cartmell, K.Huynh, A.Lin, S.Nagaraja, R.Guldberg: J. Biomed. Mater. Res. A69 (2004) 97.10.1002/jbm.a.20118Search in Google Scholar PubMed

[10] R.Bernhardt, J.van den Dolder, S.Bierbaum, R.Beutner, D.Scharnweber, J.Jansen, F.Beckmann, H.Worch: Biomaterials26 (2005) 3009.10.1016/j.biomaterials.2004.08.030Search in Google Scholar PubMed

[11] S.Rajagopalan, L.Lu, M.J.Yaszemski, R.A.Robb: J. Biomed. Mater. Res. A75 (2005) 877.Search in Google Scholar

[12] E.Skutelsky, J.Roth: J. Histochem. Cytochem.34 (1986) 693.10.1177/34.5.3701033Search in Google Scholar PubMed

[13] R.Zehbe, J.Libera, U.Gross, H.Schubert: Bio-Med. Mater. Eng.15 (2005) 445.Search in Google Scholar

[14] H.Schubert, I.Garrn, A.Berthold, W.U.Knauf, B.Reufi, T.Fietz, U.Gross: J. Mater. Sci. Mater. Med.15 (2004) 331.10.1023/B:JMSM.0000021097.29842.9fSearch in Google Scholar

[15] F.Gabler, S.Frauenschuh, J.Ringe, C.Brochhausen, P.Götz, C.J.Kirkpatrick, M.Sittinger, H.Schubert, R.Zehbe: Biomol. Eng. (2000) accepted.Search in Google Scholar

[16] R.Zehbe, C.Brochhausen, A.Haibel, C.J.Kirkpatrick, U.Gross, H.Schubert: BIOmaterialien7 (2006) 249.Search in Google Scholar

[17] S.Basu, Y.Bresler: IEEE Transactions on image Processing9 (2000) 1760.10.1109/83.869187Search in Google Scholar PubMed

[18] A.C.Kak, M.Slaney: Principles of Computerized Tomographic Imaging, IEEE Press (1988).Search in Google Scholar

[19] G.R.Owen, M.Kääb, K.Ito: Scanning Microscopy13 (1999) 83.Search in Google Scholar

[20] M.Kääb, R.G.Richards, P.Walther, I.A.Gwynn, H.P.Nötzli: Scanning Microscopy13 (1999) 61.Search in Google Scholar

[21] C.Brochhausen, P.Neuland, C.J.Kirkpatrick, R.M.Nüsing, G.Klaus: Arthritis Res. Ther.8 (2006) R78.10.1186/ar1948Search in Google Scholar PubMed PubMed Central

[22] C.Brochhausen, R.Zehbe, U.Gross, H.Schubert, C.J.Kirkpatrick: Journal of Applied Biomaterials & Biomechanics (2007) in press.Search in Google Scholar

[23] C.Brochhausen, R.Zehbe, B.Watzer, S.Halstenberg, U.Gross, H.Schubert, C.J.Kirkpatrick: Schmiedeberg Naunyn9s Arch Pharmacol (2007) accepted.Search in Google Scholar

[24] J.C.Fiala: Journal of Microscopy218 (2005) 52.10.1111/j.1365-2818.2005.01466.xSearch in Google Scholar PubMed

Published Online: 2013-05-23
Published in Print: 2007-07-01

© 2007, Carl Hanser Verlag, München

Articles in the same Issue

  1. Contents
  2. Contents
  3. Editorial
  4. Editorial
  5. Basic
  6. Diffusional decomposition of supersaturated solid solutions at grain boundaries
  7. Characterization of oriented protein-ceramic and protein-polymer-composites for cartilage tissue engineering using synchrotron μ-CT
  8. Effect of ageing on optical and thermal properties of YBa2Cu3O7–δ
  9. Calorimetric study and thermodynamic assessment of the SrO–Ga2O3 system
  10. Re-determination of transition temperatures in the Fe–Al system by differential thermal analysis
  11. Applied
  12. Novel corrosion protective coatings for aluminium alloys and steels based on oxidic nanoparticles
  13. Novel biomimetically based ice-nucleating coatings
  14. Biomimetically inspired hybrid materials based on silicified collagen
  15. Magnesium sponges as a bioabsorbable material – attributes and challenges
  16. Morphology of bony tissues and implants uncovered by high-resolution tomographic imaging
  17. Review
  18. The effects of metal implants on inflammatory and healing processes
  19. Formation of nano hydroxyapatite – a straightforward way to bioactivate bone implant surfaces
  20. Drug-eluting stent technologies for vascular regeneration
  21. Fiber structures for the regenerative medicine
  22. Stimuli-responsive polymer layers for advanced cell culture technologies
  23. Notifications
  24. Robert W. Cahn: 1924–2007
  25. DGM News
https://doi.org/10.3139/146.101509
Keywords for this article
Tissue engineering; Joint cartilage; Synchrotron μ-CT imaging; Gelatine scaffold; Porcine chondrocytes

Articles in the same Issue

  1. Contents
  2. Contents
  3. Editorial
  4. Editorial
  5. Basic
  6. Diffusional decomposition of supersaturated solid solutions at grain boundaries
  7. Characterization of oriented protein-ceramic and protein-polymer-composites for cartilage tissue engineering using synchrotron μ-CT
  8. Effect of ageing on optical and thermal properties of YBa2Cu3O7–δ
  9. Calorimetric study and thermodynamic assessment of the SrO–Ga2O3 system
  10. Re-determination of transition temperatures in the Fe–Al system by differential thermal analysis
  11. Applied
  12. Novel corrosion protective coatings for aluminium alloys and steels based on oxidic nanoparticles
  13. Novel biomimetically based ice-nucleating coatings
  14. Biomimetically inspired hybrid materials based on silicified collagen
  15. Magnesium sponges as a bioabsorbable material – attributes and challenges
  16. Morphology of bony tissues and implants uncovered by high-resolution tomographic imaging
  17. Review
  18. The effects of metal implants on inflammatory and healing processes
  19. Formation of nano hydroxyapatite – a straightforward way to bioactivate bone implant surfaces
  20. Drug-eluting stent technologies for vascular regeneration
  21. Fiber structures for the regenerative medicine
  22. Stimuli-responsive polymer layers for advanced cell culture technologies
  23. Notifications
  24. Robert W. Cahn: 1924–2007
  25. DGM News
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