Studies on Emulsification Properties of Glycolipids Biosurfactants
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Harshada Patil
Abstract
Microbial biosurfactants consists of hydrophilic and hydrophobic moieties in its structure and are produced by microorganisms. Glycolipid class of biosurfactants has wide range of surface and interfacial properties. The emulsification activity and emulsion stability of the Glycolipids Trehalose lipids (THL), Mannosylerrythritol lipids (MEL), Sophorolipids (SL) and Rhamnolipids (RL) were investigated using liquid paraffin (hydrocarbon source) and sunflower oil (vegetable source) as hydrophobic source by UV spectroscopy. Emulsification activity and stability are in the order THL > MEL > SL > RL. The stability as a function of the temperature in the range of 20 °C – 80 °C is in order THL > SL > MEL > RL. The effect of pH was studied using buffers of acidic and basic pH. It was observed that RL and SL had excellent emulsification activity at pH 8 while the activity of trehalose lipids and mannosylerrythritol lipids was not affected by pH. Similar effect of various concentrations of salt (NaCl) was studied; THL and MEL emulsion were very resistant to concentration of salt but the stability of SL and RL emulsion decreased with increased salt concentration. Average droplet diameter of emulsion and the polydispersity index were determined by dynamic light scattering. The emulsions of THL and SL have smallest droplet diameter of 422 nm and 625 nm, while emulsions of MEL and RL have a droplet size of 1923 nm and 2245 nm respectively. Emulsions of all investigatesd glycolipid surfactants had good polydispersity index and negative zeta potential, suggesting their possible applications in pharmaceutical, cosmetics, industrial and environmental techniques.
Kurzfassung
Die Moleküle mikrobieller Biotenside bestehen aus hydrophilen und hydrophoben Anteilen und werden von Mikroorganismen hergestellt. Von den Biotensiden haben Glykolipide eine breite Palette von Oberflächen- und Grenzflächeneigenschaften. Die Emulgierfähigkeit und Emulsionsstabilität der Glycolipide Trehaloselipide (THL), Mannosylerrythritollipide (MEL), Sophorolipide (SL) und Rhamnolipide (RL) wurden unter Verwendung von flüssigem Paraffin (Kohlenwasserstoffquelle) und Sonnenblumenöl (pflanzliche Quelle) als hydrophobe Quelle mittels UV-Spektroskopie untersucht. Für die Emulgierungsfähigkeit und Emulsionsstabilität ergibt sich folgende Reihenfolge: THL > MEL > SL> RL. Die Stabilität im Temperaturbereich von 20 °C – 80 °C hat folgende Reihenfolge: THL > SL > MEL > RL. Der Einfluss des pH-Werts wurde unter Verwendung von Puffer im sauren und basischen pH-Bereich untersucht. Es wurde beobachtet, dass RL und SL bei pH 8 eine ausgezeichnete Emulgierungsfähigkeit hatten, während die Fähigkeit von THL und MEL nicht durch den pH-Wert beeinflusst wurden. Ein ähnlicher Einfluss von verschiedenen Salzkonzentrationen (NaCl-Konzentrationen) wurde studiert; THL- und SL-Emulsionen blieben bei steigender Salzkonzentration stabil, wohingegen die Stabilität der SL- und RL-Emulsionen mit steigender Salzkonzentration abnahm. Der durchschnittliche Tröpfchendurchmesser der Emulsionen und Polydispersitätsindex wurden durch dynamische Lichtstreuung bestimmt. Die Emulsionen von THL und SL hatten einen kleinsten Tröpfchendurchmesser von 422 nm und 625 nm, während die Emulsionen von MEL und RL einen Tröpfchendurchmesser von 1 923 nm bzw. 2 245 nm aufweisen. Die Emulsionen aller untersuchten Glykolipidtenside hatten einen guten Polydispersitätsindex und ein negatives Zetapotential, was auf mögliche Anwendungen in der Pharmazie, Kosmetik und in der Industrie- und Umwelttechnik hindeutet.
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© 2017, Carl Hanser Publisher, Munich
Articles in the same Issue
- Contents/Inhalt
- Contents
- Detergent/Cleaning
- Consumers' Comprehension of the EU Energy Label for Washing Machines
- Effect of Washing Conditions on Cleaning Action of Linear Alkylbenzene Sulfonate in Hard Water
- Biosurfactants/Novel Surfactants
- Surface Activity Study of Water-Soluble Silk Fibroin Prepared using Cocoons and Ca(NO3)2 · 4H2O
- Experimental Design Procedure for Optimization of Saponin Extraction from Glycyrrhiza glabra: A Biosurfactant for Emulsification of Heavy Crude Oil
- Studies on Emulsification Properties of Glycolipids Biosurfactants
- Synthesis and Characterization of Saturated Cardanol Sulfonate Salt Gemini Surfactant
- Physical Chemistry
- Effect of Surface Dilatational Modulus on Foam Flow in a Porous Medium
- Oil-Water Interfacial Tensions of Silica Nanoparticle-Surfactant Formulations
- Interactions of Cationic, Anionic and Nonionic Surfactants with Cresol Red Dye in Aqueous Solutions: Conductometric, Tensiometric, and Spectroscopic Studies
- Application
- Physicochemical Properties of Amino Acid Surfactants and Their Use in Dyeing with Natural Plant Dyes
- Short Communication
- Demulsification of Water-in-Heavy Crude Oil Emulsion using Amphiphilic Ammonium Salts as Demulsifiers
Articles in the same Issue
- Contents/Inhalt
- Contents
- Detergent/Cleaning
- Consumers' Comprehension of the EU Energy Label for Washing Machines
- Effect of Washing Conditions on Cleaning Action of Linear Alkylbenzene Sulfonate in Hard Water
- Biosurfactants/Novel Surfactants
- Surface Activity Study of Water-Soluble Silk Fibroin Prepared using Cocoons and Ca(NO3)2 · 4H2O
- Experimental Design Procedure for Optimization of Saponin Extraction from Glycyrrhiza glabra: A Biosurfactant for Emulsification of Heavy Crude Oil
- Studies on Emulsification Properties of Glycolipids Biosurfactants
- Synthesis and Characterization of Saturated Cardanol Sulfonate Salt Gemini Surfactant
- Physical Chemistry
- Effect of Surface Dilatational Modulus on Foam Flow in a Porous Medium
- Oil-Water Interfacial Tensions of Silica Nanoparticle-Surfactant Formulations
- Interactions of Cationic, Anionic and Nonionic Surfactants with Cresol Red Dye in Aqueous Solutions: Conductometric, Tensiometric, and Spectroscopic Studies
- Application
- Physicochemical Properties of Amino Acid Surfactants and Their Use in Dyeing with Natural Plant Dyes
- Short Communication
- Demulsification of Water-in-Heavy Crude Oil Emulsion using Amphiphilic Ammonium Salts as Demulsifiers
