Sophorolipids Synthesized Using Non-Traditional Oils with Glycerol and Studies on Their Surfactant Properties with Synthetic Surfactant

Akash P. Bhangale; Sushand D. Wadekar; Sandeep B. Kale; Amit P. Pratap

doi:10.3139/113.110320

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Sophorolipids Synthesized Using Non-Traditional Oils with Glycerol and Studies on Their Surfactant Properties with Synthetic Surfactant

Akash P. Bhangale , Sushand D. Wadekar , Sandeep B. Kale und Amit P. Pratap

Veröffentlicht/Copyright: 25. September 2014

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Aus der Zeitschrift Tenside Surfactants Detergents Band 51 Heft 5

Abstract

The importances of bio-surfactants in industrial applications are huge due to their biodegradable and eco-friendly nature. Bio-surfactants mostly find application in cosmetics and health care products. Moreover, bio-surfactants like sophorolipids (SL) also exhibit antimicrobial and skin healing properties. The current studies involve production of SL using low cost substrates like glycerol (15%) instead of glucose (10%) with non-traditional oils (10%) such as jatropha oil, karanja oil and neem oil by using Starmerella bombicola (ATCC 22214). Neem oil gave lower yield i.e. (1.42 g/L) of SL as compared to jatropha oil (4.74 g/L) and karanja oil (5.91 g/L) with glycerol as substrate. Some pretreatment given to crude neem oil like oil refining and ethanol washing with glycerol helps in improved cell growth and SL yield i.e. 2.73 g/L and 3.82 g/L respectively. The comparison thin layer chromatography (TLC), Fourier Transform infrared spectra (FTIR), high performance liquid chromatography (HPLC), liquid chromatography mass spectra (LC-MS) and proton nuclear magnetic resonance spectroscopy (¹H NMR) of SL produced on non-traditional oils with glycerol were carried out with that of SL produced on non-traditional oils with glucose. The surfactant properties of sodium lauryl sulphate (SLS) such as surface tension, interfacial tension, stabilization of foam, emulsification, and wetting were improved when SLS was replaced at different concentration of SL.

Kurzfassung

Biotenside in industriellen Anwendungen haben große Bedeutung aufgrund ihrer biologischen Abbaubarkeit und ihrer umweltfreundlichen Eigenschaften. Biotenside findet man hauptsächlich in Kosmetik und Gesundheitsprodukten. Darüber hinaus zeigen Biotenside wie Sophorolipide (SL) antimikrobielle und hautheildende Eigenschaften. Die gegenwärtigen Untersuchungen beschäftigen sich mit der Produktion von SL, bei der preiswerte Substrate wie Glycerol (15%) statt Glucose (10%) und nicht-traditionelle Öle wie Jatrophaöl, Karanjaöl und Neemöl unter Einsatz von Starmerella bombicola (ATCC 22214) verwendet werden. Im Vergleich zu Jatrophaöl (4.74 g/L) und Karanjaöl (5.91 g/L) lieferte Neemöl (1.42 g/L) mit Glycerol als Substrat eine geringere Ausbeute an Sophorolipiden. Einige Vorbehandlungen des Rohneemöls wie Ölraffinierung und Ethanolwäsche mit Gylcerol bewirkten eine Verbesserung des Zellwachstums und der SL-Ausbeute (2.73 g/L bzw. 3.82 g/L). Mittels der Methoden FT-IR, HPLC, LC-MS und ¹H-NMR wurden SL, die aus nicht-traditonellen Ölen mit Glycerol erzeugt wurden, mit den SL verglichen, die man aus nicht-traditonellen Ölen mit Glucose erhielt. Die tensidischen Eigenschaften von Natriumlaurylsulfat (SLS) wie Grenz- und Oberflächenspannung, Schaumstabilität, Emulgierung und Benetzung wurden verbessert, wenn SLS in bestimmten Anteilen durch SL ersetzt wurde.

Key words: Sophorolipids; non-traditional oils; pretreated neem oil; surfactant properties; Starmerella bombicola (ATCC 22214)

Stichwörter: Sophorolipide; nicht-traditionelle Öle; vorbehandeltes Neemöl; tensidische Eigenschaften; Starmerella bombicola (ATCC 22214)

* Correspondence address Mr. Prof. Dr. Amit Patrap, Department of Oils, Oleochemicals and Surfactants Technology, Institute of Chemical Technology, (University under Section 3 of UGC Act 1956; Formerly UDCT/UICT), Nathalal Parekh Marg, Matunga (East), Mumbai – 400 019, India. E-Mail: amitpratap0101@rediffmail.com

Dr. Amit P. Pratap completed his graduation and post graduation in Oil Technology in 2001 and obtained his doctorate degree in 2006 from Institute of Chemical Technology, Mumbai. He served the department as a ‘Professor J. G. Kane Academic Associate’ for over two years and at present he is working as Assistant Professor. For the past eight years he is involved in the teaching, research and development in the field of vegetable oil based lubricants, additives and biosurfactants. His research interest includes triboapplications of vegetable oils, structural modifications of oils and fats, biosurfactants and specialty products.

Dr. S. B. Kale completed his graduation in Pharmaceutical Sciences and post graduation in Bioprocess Technology from ICT, Mumbai and obtained his doctorate degree in the field of Biotechnology from ICT, Mumbai. He is working as Assistant Professor in DBT-ICT Centre for Energy Biosciences from past 5 years. His research interest includes biotransformation, fermentation, chromatographic separation etc.

Mr. S. D. Wadekar completed his graduation and post graduation in Oil Technology in 2006 from Department of Oils, Oleochemicals and Surfactants Technology, Institute of Chemical Technology, Mumbai. He is completed his doctoral programme from Oils Dept. at ICT.

Mr. A. P. Bhangale completed his post graduation in Oil Technology from LIT, Nagpur and at present working for his doctoral programme in Department of Oils, Oleochemicals and Surfactants Technology, Institute of Chemical Technology, Mumbai.

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Received: 2014-01-28

Revised: 2014-05-12

Published Online: 2014-09-25

Published in Print: 2014-09-15

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Artikel in diesem Heft

https://doi.org/10.3139/113.110320

Schlagwörter für diesen Artikel

Sophorolipids; non-traditional oils; pretreated neem oil; surfactant properties; Starmerella bombicola (ATCC 22214)