Study of Glycerol Residue as a Carbon Source for Production of Rhamnolipids by Pseudomonas aeruginosa (ATCC 10145)

S. D. Wadekar; S. V. Patil; S. B. Kale; A. M. Lali; D. N. Bhowmick; A. P. Pratap

doi:10.3139/113.110098

Article

Study of Glycerol Residue as a Carbon Source for Production of Rhamnolipids by Pseudomonas aeruginosa (ATCC 10145)

S. D. Wadekar , S. V. Patil , S. B. Kale , A. M. Lali , D. N. Bhowmick and A. P. Pratap

Published/Copyright: April 5, 2013

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From the journal Tenside Surfactants Detergents Volume 48 Issue 1

Abstract

Rhamnolipid is the simplest class of biosurfactants with a well defined structure. The main obstacle in commercialization of biosurfactant especially rhamnolipids is the higher cost of the production. The production cost can be reduced by using economical carbon sources. Glycerol residue is available from glycerin distillation plant at low cost. It can be used as carbon source for rhamnolipid production using Pseudomonas aeruginosa (ATCC 10145). The optimum concentration of glycerol residue was 5% weight by volume (w/v) yielding maximum rhamnolipids at 2.50 g/L in the broth. From batch monitoring of polyglycerol and monoglycerol consumption, it appeared that polyglycerols were first hydrolyzed to monoglycerol in the broth itself and consumed as carbon source. The product had components similar to rhamnolipids synthesized on pure glycerol under the same conditions. This indicated the potential of glycerol residue as economic substrate for production of rhamnolipids.

Kurzfassung

Rhamnolipid gehört zu der einfachsten Klasse von Biotensiden mit gut bekannter Struktur. Das größte Problem bei der kommerziellen Herstellung von Biotensiden speziell der Rhamnolipide sind die hohen Produktionskosten. Diese Kosten können durch Einsatz von wirtschaftlichen Kohlenstoffquellen reduziert werden. Glyzerinrückstände stehen kostengünstig aus Glyzerindestillationen von Pflanzen zur Verfügung. Sie können als Kohlenstoffquelle für die Rhamnolipidproduktion unter Verwendung von Pseudomonas aeruginosa (ATCC 10145) genutzt werden. Die optimale Konzentration des Glyzerinrückstandes von 5% (w/v) lieferte maximal 2.5 g/L Rhamnolipide in der Lösung. Die Serienuntersuchung des Polyglyzerin- und Monoglyzerinverbrauchs zeigte, dass Polyglyzerin zuerst zu Monoglyzerin in der Lösung hydrolysiert und als Kohlenstoffquelle verbraucht wurde. Das Produkt hatte Komponenten ähnlich denen von Rhamnolipiden, die in reinem Glyzerin unter gleichen Bedingungen synthetisiert wurden. Dieses Ergebnis belegt das Potenzial von Glyzerinrückständen als Substrat für die wirtschaftliche Herstellung von Rhanmolipiden.

Key words:: Rhamnolipids; Pseudomonas aeruginosa; Glycerol residue; batch processing; optimization

Stichwörter:: Rhamnolipide; Pseudomonas aeruginosa; Glyzerinrückstände; Batch-Verfahren; Optimierung

^∗ Dr. Amit P. Pratap, Assistant Professor, Department of Oils, Oleochemicals and Surfactants Technology, Institute of Chemical Technology, (University under Section 3 of UGC Act 1956; Formerly UDCT/UICT), Nathalal Parekh Marg, Matunga (East), Mumbai – 400 019 India, Tel.: +91-22-33611111/2222 Ext. 2557, Fax: +91-22-33611020. E-Mail: amitpratap0101@rediffmail.com, ap.pratap@ictmumbai.edu.in, Web: www.ictmumbai.org

S. D. Wadekar completed his graduation and post graduation in Oil Technology in 2006 from Department of Oils, Oleochemicals and Surfactants Technology, Institute of Chemical Technology, Mumbai. At present he is working for his doctoral programme in the Oils Dept. at ICT.

S. V. Patil completed his graduation in Oil Technology in 2008 from Department of Oils, Oleochemicals and Surfactants Technology, North Maharashtra University Jalgaon. At present he is working for his M. Tech. degree in the Oils Dept. at ICT.

S. B. Kale completed his graduation in Pharmaceutical Sciences and post graduation in Bioprocess Technology from ICT, Mumbai and obtained his doctorate degree in the field of Biotechnology from ICT, Mumbai. He is working as lecturer in DBT-ICT Centre for Energy Biosciences from past 2 years. His research interest includes biotransformation, fermentation, chromatographic separation etc.

A. M. Lali completed his graduation and post graduation in Chemical Engineering from ICT, Mumbai and obtained his doctorate degree in the field of multiphase reactors from ICT, Mumbai. He is working as Professor in Chemical Engineering Department and also serving the institute as Co-ordinator, DBT-ICT Centre for Energy Biosciences. For the past twenty years he is involved in the teaching, research and development in the field of biotechnology, protein purification, biotransformation, fermentation, chromatographic separation etc.

D. N. Bhowmick completed his graduation and post graduation in Oil Technology from HBTI, Kanpur his doctorate degree from Indian Institute of Technology (IIT), Mumbai. He was heading the department for over 10 years and at present he is working as a Dean (Academic programme). For the past twenty five years he is involved in the teaching, research and development in the field of membrane technology, and specialty products.

Dr. Amit P. Pratap completed his graduation and post graduation in Oil Technology in 2001 and obtained his doctorate degree in 2006 from Institute of Chemical Technology, Mumbai. He served the department as a “Professor J. G. Kane Academic Associate” for over two years and at present he is working as a Lecturer (Senior Scale). For the past seven years he is involved in the teaching, research and development in the field of vegetable oil based lubricants, additives and biosurfactants. His research interest includes triboapplications of vegetable oils, structural modifications of oils and fats, biosurfactants, and specialty products.

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Received: 2010-09-14

Published Online: 2013-04-05

Published in Print: 2011-01-01

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Keywords for this article

Rhamnolipids; Pseudomonas aeruginosa; Glycerol residue; batch processing; optimization