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Evaluation of antioxidant activity and DNA cleavage protection effect of naphthyl hydroxamic acid derivatives through conventional and fluorescence microscopic methods

  • Priyanka Singh EMAIL logo , Deepesh Khare and Rama Pande
Published/Copyright: June 24, 2014
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Chemical Papers
From the journal Chemical Papers Volume 68 Issue 10

Abstract

Antioxidant capacity of N-(1-naphthyl)valerohydroxamic acid (NVHA) and N-(1-naphthyl)phenylacetohydroxamic acid (NPAHA) has been evaluated by a novel approach employing the fluorescence microscopic single molecule observation method. This method allows direct observation of the changes in single DNA molecules. The DNA cleavage protection activity of the compounds was also assessed by the gel electrophoresis method. The applied methods confirmed that both compounds are capable of inhibiting the free radical mediated DNA damage. Free radical scavenging activity was assessed via the 2,2′-diphenyl-1-picrylhydrazyl free radical (DPPH) and lipid peroxidation inhibition methods. The effective concentration causing a 50 % inhibition of the DPPH concentration, EC50, was found to be 371.54 mM for NVHA and 365.95 mM for NPAHA. Its lipid peroxidation inhibition ability was calculated to be 40.91 % at 371.54 mM for NVHA and 41.14 % at 365.95 mM for NPAHA. These results show the antioxidant potential of the naphthyl hydroxamic acids.

Keywords: DNA cleavage protection; fluorescence microscope; free radical scavenging activity; naphthyl hydroxamic acid

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Published Online: 2014-6-24
Published in Print: 2014-10-1

© 2014 Institute of Chemistry, Slovak Academy of Sciences

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  2. Evaluation of antioxidant activity and DNA cleavage protection effect of naphthyl hydroxamic acid derivatives through conventional and fluorescence microscopic methods
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https://doi.org/10.2478/s11696-014-0576-0
Keywords for this article
DNA cleavage protection; fluorescence microscope; free radical scavenging activity; naphthyl hydroxamic acid

Articles in the same Issue

  1. Chemical preparation and applications of silver dendrites
  2. Evaluation of antioxidant activity and DNA cleavage protection effect of naphthyl hydroxamic acid derivatives through conventional and fluorescence microscopic methods
  3. Modelling of ORL1 receptor-ligand interactions
  4. Kinetics of enantioselective liquid-liquid extraction of phenylglycine enantiomers using a BINAP-copper complex as chiral selector
  5. Diffusive transport of Cu(II) ions through thin ion imprinted polymeric membranes
  6. Magnetic mixed matrix membranes in air separation
  7. The use of impregnated perlite as a heterogeneous catalyst for biodiesel production from marula oil
  8. Nitrobenzene degradation by micro-sized iron and electron efficiency evaluation
  9. RP-HPLC-UV-ESI-MS phytochemical analysis of fruits of Conocarpus erectus L.
  10. Residue analysis of fosthiazate in cucumber and soil by QuEChERS and GC-MS
  11. Degradation of polylactide using basic ionic liquid imidazolium acetates
  12. Ring-opening polymerisation of ɛ-caprolactone catalysed by Brønsted acids
  13. Click synthesis by Diels-Alder reaction and characterisation of hydroxypropyl methylcellulose-based hydrogels
  14. Preparation and physical properties of chitosan-coated calcium sulphate whiskers
  15. A facile synthetic route for antineoplastic drug GDC-0449
  16. Two new frameworks for biphenyl-3,3′,5,5′-tetracarboxylic acid and nitrogen-containing organics
  17. Antioxidant and binding properties of methanol extracts from indigo plant leaves
  18. Dithiols as more effective than monothiols in protecting biomacromolecules from free-radical-mediated damage: in vitro oxidative degradation of high-molar-mass hyaluronan
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