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Improvement in γ-decalactone production by Yarrowia sp. after genome shuffling

  • Yu-Ping Zhao EMAIL logo , Xiao-Qing Mu und Yan Xu
Veröffentlicht/Copyright: 15. April 2014
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Chemical Papers
Aus der Zeitschrift Chemical Papers Band 68 Heft 8

Abstract

In this study, a modified genome shuffling method was used to improve γ-decalactone (GDL) production of Yarrowia sp. China General Microbiological Culture Collection Center (CGMCC 2.1405). Five UV mutant strains with higher GDL production or shorter fermentation time were selected as the initial strains for genome shuffling. Conditions of protoplast preparation, regeneration, inactivation, fusion, sporulation of recombinant fusants and ascospore isolation were optimized. Four hereditarily stable haploid recombinants with high GDL production were obtained by three rounds of genome shuffling. Among them, a high GDL-producing recombinant, G3-3.21, producing 3.75 g L−1 of GDL in the fermentation medium after 64 h was obtained. This value is 6.54-fold higher than that of the parent strains CGMCC 2.1405, at the peak production shortened by 8 h. Mathematical kinetic models of CGMCC 2.1405 and G3-3.21 were established to well predict the cell growth and GDL production. The cell growth of G3-3.21 was significantly faster than that of CGMCC 2.1405. The product synthesis constant associated with the strain growth of G3-3.21 was higher than that associated with CGMCC 2.1405. Long-chain fatty-acyl-CoA oxidase activities of G3-3.21 were 833 mU mg−1 and 6.83 times higher than that of CGMCC 2.1405.

Keywords: γ-decalactone; Yarrowia sp.; cell morphology; protoplast formation; genome shuffling

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Published Online: 2014-4-15
Published in Print: 2014-8-1

© 2014 Institute of Chemistry, Slovak Academy of Sciences

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  7. Effect of anions on the structure and catalytic properties of a La-doped Cu-Mn catalyst in the water-gas shift reaction
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https://doi.org/10.2478/s11696-014-0551-9
Schlagwörter für diesen Artikel
γ-decalactone; Yarrowia sp.; cell morphology; protoplast formation; genome shuffling

Artikel in diesem Heft

  1. Recent advances in application of liquid-based micro-extraction: A review
  2. Determination of nitrites and nitrates in drinking water using capillary electrophoresis
  3. Comparison of digestion methods for determination of total phosphorus in river sediments
  4. Interdisciplinary study on pottery experimentally impregnated with wine
  5. Improvement in γ-decalactone production by Yarrowia sp. after genome shuffling
  6. Development of an effective extraction process for coenzyme Q10 from Artemia
  7. Effect of anions on the structure and catalytic properties of a La-doped Cu-Mn catalyst in the water-gas shift reaction
  8. Effect of apple pomace powder addition on farinographic properties of wheat dough and biscuits quality
  9. Influence of caffeine and temperature on corrosion-resistance of CoCrMo alloy
  10. Cetyltrimethylammonium bromide- and ethylene glycol-assisted preparation of mono-dispersed indium oxide nanoparticles using hydrothermal method
  11. Sol-gel synthesis, characterisation, and photocatalytic activity of porous spinel Co3O4 nanosheets
  12. Solvent-free synthesis of β-enamino ketones and esters catalysed by recyclable iron(III) triflate
  13. Potassium phthalimide-catalysed one-pot multi-component reaction for efficient synthesis of amino-benzochromenes in aqueous media
  14. Organocatalytic SOMO reactions of copper(I)-acetylide and alkylindium compounds with aldehydes
  15. Molecular modelling and quantitative structure-activity relationship studies of anatoxin-a and epibatidine derivatives with affinity to rodent nAChR receptors
  16. Efficient one-pot synthesis of 2-hydroxyethyl per-O-acetyl glycosides
  17. Properties of singlet- and triplet-excited states of hemicyanine dyes
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