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Modeling of equilibrium and kinetics of human polyclonal immunoglobulin G adsorption on a tentacle cation exchanger

  • Katarzyna Wrzosek EMAIL logo , Pavel Ačai , Michal Gramblička and Milan Polakovič
Published/Copyright: August 21, 2013
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Chemical Papers
From the journal Chemical Papers Volume 67 Issue 12

Abstract

Adsorption of human immunoglobulin G (IgG) on a commercial cation exchanger with a grafted polymer layer was investigated at pH 4.5 and in the NaCl concentration range of 0–150 mM. Adsorption equilibrium was determined in static batch experiments and verified in batch uptake experiments. Parameters of the Langmuir isotherm were estimated for each salt concentration separately. The batch uptake experiments provided also the estimates of effective pore diffusion coefficients of IgG for individual protein and salt concentrations. The values of the effective pore diffusion coefficient depended strongly on both factors. They increased by about 5–15 times with the NaCl concentration and decreased about three times with the protein concentration. The quality of the estimated parameters was confirmed by frontal experiments described by the general rate model of chromatography.

Keywords: cation-exchange chromatography; grafted tentacle layer; IgG adsorption; Langmuir isotherm; effective pore diffusion coefficient; general rate model

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Published Online: 2013-8-21
Published in Print: 2013-12-1

© 2013 Institute of Chemistry, Slovak Academy of Sciences

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https://doi.org/10.2478/s11696-013-0421-x
Keywords for this article
cation-exchange chromatography; grafted tentacle layer; IgG adsorption; Langmuir isotherm; effective pore diffusion coefficient; general rate model

Articles in the same Issue

  1. Fifty years of the Department of Chemical and Biochemical Engineering at the Slovak University of Technology in Bratislava — brief history of research
  2. Airlift reactor — membrane extraction hybrid system for aroma production
  3. CFD-based atmospheric dispersion modeling in real urban environments
  4. Catalytic gasification of pyrolytic oil from tire pyrolysis process
  5. Kinetics of thermal decomposition of aseptic packages
  6. Sulphur distribution in the products of waste tire pyrolysis
  7. Equilibrium and kinetics of protein binding on ion-exchange cellulose membranes with grafted polymer layer
  8. Modeling of equilibrium and kinetics of human polyclonal immunoglobulin G adsorption on a tentacle cation exchanger
  9. Design calculations of an extractor for aromatic and aliphatic hydrocarbons separation using ionic liquids
  10. Effect of viscosity of a liquid membrane containing oleyl alcohol on the pertraction of butyric acid
  11. Anaerobic treatment of rapeseed meal
  12. Anoxic granulated biomass and its storage
  13. Removal of selected chlorinated micropollutants by ozonation
  14. Degradation and toxicity changes in aqueous solutions of chloroacetic acids by Fenton-like treatment using zero-valent iron
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