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Endothelial cell adhesion on polyelectrolyte multilayer films functionalised with fibronectin and collagen

  • Nahla Zanina EMAIL logo , Soumaya Haddad , Ali Othmane , Thierry Jouenne , David Vaudry , Mina Souiri and Laurence Mora
Published/Copyright: April 5, 2012
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Chemical Papers
From the journal Chemical Papers Volume 66 Issue 5

Abstract

The seeding of endothelial cells on biomaterial surfaces has become a major challenge to achieve better haemocompatibility of these surfaces. Multilayers of polyelectrolytes formed by the layerby-layer method are promising in this respect. In this study, the interactions of endothelial cells with multilayered polyelectrolytes films were investigated. The build-ups were prepared by selfassembled alternatively adsorbed polyanions and polycations functionalised with fibronectin and collagen. Anionic poly(sodium 4-styrenesulfonate) and cationic poly(allylamine hydrochloride) polyelectrolytes were chosen as a model system. Elaborated surfaces were characterised by electrochemical impedance spectroscopy and cyclic voltammetry. The modified electrode showed good reversible electrochemical properties and high stability in an electrolyte solution. The film ohmic resistance was highest when the film was coated with fibronectin; the parameters so determined were correlated with atomic force microscopy images. Cell colorimetric assay (WST-1) and immunofluorescence were used to quantify the cell viability and evaluate the adhesion properties. When cultured on a surface where proteins were deposited, cells adhered and proliferated better with fibronectin than with collagen. In addition, a high surface free energy was favourable to adhesion and proliferation (48.8 mJ m−2 for fibronectin and 39.7 mJ m−2 for collagen, respectively). Endothelial cells seeded on functionalised-polyelectrolyte multilayer films showed a good morphology and adhesion necessary for the development of a new endothelium.

Keywords: polyelectrolyte multilayers; endothelial cells; cell adhesion; proliferation; atomic force microscopy; wettability; electrochemical impedance spectroscopy

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Published Online: 2012-4-5
Published in Print: 2012-5-1

© 2012 Institute of Chemistry, Slovak Academy of Sciences

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https://doi.org/10.2478/s11696-012-0141-7
Keywords for this article
polyelectrolyte multilayers; endothelial cells; cell adhesion; proliferation; atomic force microscopy; wettability; electrochemical impedance spectroscopy

Articles in the same Issue

  1. Ultrathin organic, inorganic, hybrid, and living cell coatings — Topical Issue
  2. Functional polymer thin films designed for antifouling materials and biosensors
  3. In-situ polymerized molecularly imprinted polymeric thin films used as sensing layers in surface plasmon resonance sensors: Mini-review focused on 2010–2011
  4. Design of polyglycidol-containing microspheres for biomedical applications
  5. On the interfacial chemistry of aryl diazonium compounds in polymer science
  6. Polypyrrole coating of inorganic and organic materials by chemical oxidative polymerisation
  7. Spectroscopy of thin polyaniline films deposited during chemical oxidation of aniline
  8. Ultrathin functional films of titanium(IV) oxide
  9. Sol-gel thin films with anti-reflective and self-cleaning properties
  10. Nanostructured electrocatalysts immobilised on electrode surfaces and organic film templates
  11. Influence of adsorbed oxygen on charge transport and chlorine gas-sensing characteristics of thin cobalt phthalocyanine films
  12. Ni-W alloy coatings deposited from a citrate electrolyte
  13. Role of reactive species in processing materials at laboratory temperature by spray plasma devices
  14. Electrodeposition of hafnium and hafnium-based coatings in molten salts
  15. Role of interfacial chemistry on the rheology and thermo-mechanical properties of clay-polymer nanocomposites for building applications
  16. Endothelial cell adhesion on polyelectrolyte multilayer films functionalised with fibronectin and collagen
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