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Determination of curcuminoids in substances and dosage forms by cyclodextrin-mediated capillary electrophoresis with diode array detection

  • Katarína Maráková EMAIL logo , Peter Mikuš , Juraj Piešťanský and Emil Havránek
Published/Copyright: May 21, 2011
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Chemical Papers
From the journal Chemical Papers Volume 65 Issue 4

Abstract

The present work illustrates the potential of the capillary zone electrophoresis (CZE) separation technique coupled with the on-capillary diode array detector (DAD) for highly reliable determination of curcuminoids (curcumin, CUR, demethoxycurcumin, DCUR, and bisdemethoxycurcumin, BCUR) in substances (commercially available plant extract) and pharmaceutical preparation (commercial pharmaceutical capsules) with minimal sample preparation; (2-hydroxypropyl)-β-cyclodextrin (HP-β-CD) was chosen for an anionic separation of CUR and its structural analogues (DCUR and BCUR) as an appropriate complexing agent (i) providing complete resolution of the curcuminoids and (ii) reducing adsorption of these hydrophobic analytes onto the capillary wall. DAD detection was utilised for characterisation of the composition of the separated zones via differences in the corresponding UV-VIS spectra (scanned at interval of 200–800 nm). Reference and real spectra of the analytes demonstrated that the proposed separation method was sufficiently selective to produce well-separated (i.e. spectrally homogeneous) analyte zones with no interfering compounds present. Successful validation and application of the CZE-DAD method proposed here suggest its routine use in highly effective and reliable analysis of curcuminoids in pharmaceutical samples.

Keywords: capillary zone electrophoresis; diode array detection; curcuminoids; cyclodextrin; pharmaceutical analysis

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Published Online: 2011-5-21
Published in Print: 2011-8-1

© 2011 Institute of Chemistry, Slovak Academy of Sciences

Articles in the same Issue

  1. Lipid retention of novel pressurized extraction vessels as a function of the number of static and flushing cycles, flush volume, and flow rate
  2. Determination of curcuminoids in substances and dosage forms by cyclodextrin-mediated capillary electrophoresis with diode array detection
  3. Interaction of Moringa oleifera seed lectin with humic acid
  4. Hybrid process scheme for the synthesis of ethyl lactate: conceptual design and analysis
  5. Zinc catalyst recycling in the preparation of (all-rac)-α-tocopherol from trimethylhydroquinone and isophytol
  6. Denitrification of simulated nitrate-rich wastewater using sulfamic acid and zinc scrap
  7. Anaerobic treatment of biodiesel by-products in a pilot scale reactor
  8. Preparation of magnesium hydroxide from nitrate aqueous solution
  9. Impact of the type of anodic film formed and deposition time on the characteristics of porous anodic aluminium oxide films containing Ni metal
  10. Synthesis and crystal and molecular structures of N,N′-methylenedipyridinium tetrachlorozincate(II) and N,N′-methylenedipyridinium tetrachlorocadmate(II)
  11. Effects of denaturing acid on the self-association behaviour of poly(ethylene glycol)-block-poly(γ-benzyl l-glutamate)-graft-poly(ethylene glycol) copolymer in ethanol
  12. Properties of poly(γ-benzyl l-glutamate) membrane modified by polyurethane containing carboxyl group
  13. Theoretical thermo-optical patterns relevant to glass crystallisation
  14. Morphology dependence of 1,2-diphenylethylenediamine-derived organogelator templates in solvents and their influence in the production of nanostructured silica
  15. Ferric hydrogensulphate as a recyclable catalyst for the synthesis of fluorescein derivatives
  16. An alternative synthetic process of p-acetaminobenzenesulfonyl chloride through combined chlorosulfonation by HClSO3 and PCl5
  17. An efficient and novel one-pot synthesis of 2,4,5-triaryl-1H-imidazoles catalyzed by UO2(NO3)2·6H2O under heterogeneous conditions
  18. Stereoselective synthesis of the polar part of mycestericins E and G
  19. A regio- and stereoselective three-component synthesis of 5-(trifluoromethyl)-4,5,6,7-tetrahydro-[1,2,4]triazolo[1,5-a]pyrimidine derivatives under solvent-free conditions
  20. Precautions in using global kinetic and thermodynamic models for characterization of drug release from multivalent supports
  21. A sandwich anion receptor by a BODIPY dye bearing two calix[4]pyrrole units
  22. What causes iron-sulphur bonds in active sites of one-iron superoxide reductase and two-iron superoxide reductase to differ?
  23. MTD-PLS and docking study for a series of substituted 2-phenylindole derivatives with oestrogenic activity
https://doi.org/10.2478/s11696-011-0043-0
Keywords for this article
capillary zone electrophoresis; diode array detection; curcuminoids; cyclodextrin; pharmaceutical analysis

Articles in the same Issue

  1. Lipid retention of novel pressurized extraction vessels as a function of the number of static and flushing cycles, flush volume, and flow rate
  2. Determination of curcuminoids in substances and dosage forms by cyclodextrin-mediated capillary electrophoresis with diode array detection
  3. Interaction of Moringa oleifera seed lectin with humic acid
  4. Hybrid process scheme for the synthesis of ethyl lactate: conceptual design and analysis
  5. Zinc catalyst recycling in the preparation of (all-rac)-α-tocopherol from trimethylhydroquinone and isophytol
  6. Denitrification of simulated nitrate-rich wastewater using sulfamic acid and zinc scrap
  7. Anaerobic treatment of biodiesel by-products in a pilot scale reactor
  8. Preparation of magnesium hydroxide from nitrate aqueous solution
  9. Impact of the type of anodic film formed and deposition time on the characteristics of porous anodic aluminium oxide films containing Ni metal
  10. Synthesis and crystal and molecular structures of N,N′-methylenedipyridinium tetrachlorozincate(II) and N,N′-methylenedipyridinium tetrachlorocadmate(II)
  11. Effects of denaturing acid on the self-association behaviour of poly(ethylene glycol)-block-poly(γ-benzyl l-glutamate)-graft-poly(ethylene glycol) copolymer in ethanol
  12. Properties of poly(γ-benzyl l-glutamate) membrane modified by polyurethane containing carboxyl group
  13. Theoretical thermo-optical patterns relevant to glass crystallisation
  14. Morphology dependence of 1,2-diphenylethylenediamine-derived organogelator templates in solvents and their influence in the production of nanostructured silica
  15. Ferric hydrogensulphate as a recyclable catalyst for the synthesis of fluorescein derivatives
  16. An alternative synthetic process of p-acetaminobenzenesulfonyl chloride through combined chlorosulfonation by HClSO3 and PCl5
  17. An efficient and novel one-pot synthesis of 2,4,5-triaryl-1H-imidazoles catalyzed by UO2(NO3)2·6H2O under heterogeneous conditions
  18. Stereoselective synthesis of the polar part of mycestericins E and G
  19. A regio- and stereoselective three-component synthesis of 5-(trifluoromethyl)-4,5,6,7-tetrahydro-[1,2,4]triazolo[1,5-a]pyrimidine derivatives under solvent-free conditions
  20. Precautions in using global kinetic and thermodynamic models for characterization of drug release from multivalent supports
  21. A sandwich anion receptor by a BODIPY dye bearing two calix[4]pyrrole units
  22. What causes iron-sulphur bonds in active sites of one-iron superoxide reductase and two-iron superoxide reductase to differ?
  23. MTD-PLS and docking study for a series of substituted 2-phenylindole derivatives with oestrogenic activity
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