Separation and purification of no-carrier-added arsenic from bulk amounts of germanium for use in radiopharmaceutical labelling
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M. Jahn
Abstract
Radioarsenic labelled radiopharmaceuticals could add special features to molecular imaging with positron emission tomography (PET). For example the long physical half-lives of 72As (T1/2=26 h) and 74As (T1/2=17.8 d) in conjunction with their high positron branching rates of 88% and 29%, respectively, allow the investigation of slow physiological or metabolical processes, like the enrichment and biodistribution of monoclonal antibodies in tumour tissue or the characterization of stem cell trafficking. A method for separation and purification of no-carrier-added (nca) arsenic from irradiated metallic germanium targets based on distillation and anion exchange is developed. It finally converts the arsenic into an *As(III) synthon in PBS buffer and pH 7 suitable for labelling of proteins via As-S bond formations. The method delivers radioarsenic in high purity with separation factors of 106 from germanium and an overall yield from target to labelling synthon of >40%. In a proof-of-principle experiment, the monoclonal antibody Bevacizumab, directed against the human VEGF receptor, was labelled with a radiochemical yield >90% within 1 h at room temperature with nca 72/74/77As.
© by Oldenbourg Wissenschaftsverlag, Mainz, Germany
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- Effect of solvent on the extraction of lanthanides with picrolonic acid
- Separation and purification of no-carrier-added arsenic from bulk amounts of germanium for use in radiopharmaceutical labelling
- Labelling and optimization of PHOTOFRIN® with 99mTc
- Appreciation to Referees
Artikel in diesem Heft
- New cross section measurements for the production of the Auger electron emitters 77Br and 80mBr
- Development of a SISAK extraction system for chemical studies of element 108, hassium
- Spectroscopic study on the mononuclear hydrolysis species of Pu(VI) under oxidation conditions
- Origin assessment of uranium ore concentrates based on their rare-earth elemental impurity pattern
- Performance of the mesh-type liquid cadmium cathode structure for the electrodeposition of uranium from the molten salt
- Temperature effect on U(VI) sorption onto Na-bentonite
- Rapid separation method for actinides in emergency soil samples
- Effect of solvent on the extraction of lanthanides with picrolonic acid
- Separation and purification of no-carrier-added arsenic from bulk amounts of germanium for use in radiopharmaceutical labelling
- Labelling and optimization of PHOTOFRIN® with 99mTc
- Appreciation to Referees
