Labelling and optimization of PHOTOFRIN® with 99mTc
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M. Fakhar-e-Alam
Abstract
PHOTOFRIN® was labelled with 99mTc using SnCl2·2H2O as reducing agent. Instant thin layer chromatography (ITLC-SG) in 0.05 M NaOH was used for evaluation of radiochemical purity. Labelling efficiency was dependent on various factors that include the ligand/reductant ratio, pH and time of incubation. Therefore, optimum conditions of labelling were also determined. The stability of 99mTc-PHOTOFRIN® in serum was checked by using fresh human serum. Tissue distribution of 99mTc-PHOTOFRIN® was evaluated in Sprague Dawley rats.
PHOTOFRIN® was labelled with an efficiency of >95% under optimum conditions, which were PHOTOFRIN®: 200 μg, pH: 3–4, SnCl2·2H2O: 15 μg and 30 min incubation at room temperature. The 99mTc-labelled PHOTOFRIN® remained stable in human serum for 24 h. Biodistribution study in rats revealed maximum concentration of the labelled compound in liver, lungs and spleen at 0.5 h, and significant activity was also seen in the bladder and urine, indicating the mode of urinary excretion of PHOTOFRIN®.
© by Oldenbourg Wissenschaftsverlag, Islamabad 45650, Germany
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- Labelling and optimization of PHOTOFRIN® with 99mTc
- Appreciation to Referees
Articles in the same Issue
- New cross section measurements for the production of the Auger electron emitters 77Br and 80mBr
- Development of a SISAK extraction system for chemical studies of element 108, hassium
- Spectroscopic study on the mononuclear hydrolysis species of Pu(VI) under oxidation conditions
- Origin assessment of uranium ore concentrates based on their rare-earth elemental impurity pattern
- Performance of the mesh-type liquid cadmium cathode structure for the electrodeposition of uranium from the molten salt
- Temperature effect on U(VI) sorption onto Na-bentonite
- Rapid separation method for actinides in emergency soil samples
- Effect of solvent on the extraction of lanthanides with picrolonic acid
- Separation and purification of no-carrier-added arsenic from bulk amounts of germanium for use in radiopharmaceutical labelling
- Labelling and optimization of PHOTOFRIN® with 99mTc
- Appreciation to Referees
