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Spectroscopic studies on the interaction of U(VI) with Bacillus sphaericus
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Petra J. Panak
Veröffentlicht/Copyright:
25. September 2009
Summary
We studied the interaction of U(VI) with vegetative cells, heat killed cells, spores, and decomposed cells of Bacillus sphaericus. The characterization of the formed complexes was performed by time-resolved laser fluorescence spectroscopy (TRLFS) and extended X-ray absorption fine structure spectroscopy (EXAFS). We observed no significant differences in the sorption behavior of vegetative and heat killed cells, whereas the spores showed a higher sorption of U(VI) (related to their dry weight). Regardless of the higher relative sorption of the spores of B. sphaericus, the fluorescence and EXAFS spectra of the vegetative cells, heat killed cells and spores were almost identical. Analysis of the data proved that U(VI) forms inner sphere complexes with organic bound phosphate groups on the cell surface. We observed no significant differences in the coordination numbers and the distances of the oxygen and phosphorus atoms in the inner coordination sphere.
After eight weeks, the vegetative cells of B. sphaericus were completely decomposed. Lysing of the cell walls and activity of enzymes led to a release of various decomposition products. We found that large amounts of H2PO4− were released which caused a quantitative precipitation of bacterial U(VI) as UO2(H2PO4)2. The H2PO4− was detected by Raman spectroscopy. The decomposed bacterial suspension showed the same fluorescence spectrum as UO2(H2PO4)2 which differed significantly from those of the bacterial U(VI) surface complexes.
Received: 2001-9-11
Accepted: 2002-4-11
Published Online: 2009-9-25
Published in Print: 2002-9-1
© Oldenbourg Wissenschaftsverlag, München
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Artikel in diesem Heft
- Solubility of amorphous Th(IV) hydroxide – application of LIBD to determine the solubility product and EXAFS for aqueous speciation
- Thermodynamics of the U(VI)-Ca2+-Cl −-OH −-H2O system: Solubility product of becquerelite
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- Coprecipitation of thorium with UO2
- Formation of secondary phases after long-term corrosion of simulated HLW glass in brine solutions at 190 °C
- Sorption behaviour of Am on precorroded HLW glass in water and brines
- Molecular fluorescence spectroscopy and mixture analysis for the evaluation of the complexation between humic acid and UO22+
- Investigation on uranyl interaction with bioactive ligands. Synthesis and structural studies of the uranyl complexes with glycine and N-(2-mercaptopropionyl)glycine
- Complex formation of calcium with humic acid and polyacrylic acid
- Complexation of americium with humic, fulvic and citric acids at high ionic strength
- Complex formation of Eu(III) with humic acid and polyacrylic acid
- XAS study of technetium(IV) polymer formation in mixed sulphate/chloride media
- Complexation of uranium(VI) with malonate at variable temperatures
- The hydrolysis of uranium(VI)
- Corrosion and dissolution studies of UO2 containing α-emitters
- The effects of alpha-radiolysis on UO2 dissolution determined from electrochemical experiments with 238Pu-doped UO2
- Radiolytic formation of Tc(IV) oxide colloids
- Radiation-chemical effects in the near-field of a final disposal site: role of bromine on the radiolytic processes in NaCl-solutions
- The reduction of U(VI) on corroded iron under anoxic conditions
- New selenium solution speciation method by ion chromatography + gamma counting and its application to FeS2-controlled reducing conditions
- Uptake of oxo-anions by cements through solid-solution formation: experimental evidence and modelling
- Iodine species uptake by cement and CSH studied by I K-edge X-ray absorption spectroscopy
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- Uptake mechanism of IO3− to NO3−-hydrotalcite
- Structure alteration of C-S-H (calcium silicate hydrated phases) caused by sorption of caesium
- The effect of α-isosaccharinic acid on the stability of and Th(IV) uptake by hardened cement paste
- Complexation studies of Eu(III) with polyacrylic acid either free in solution or adsorbed onto alumina
- Sorption behaviour of caesium on a bentonite sample
- Specific interaction of cesium with the surface of calcium silicate hydrates
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- Instrumental development for spectroscopic speciation of f-elements in hydrothermal solutions: luminescence properties of lanthanide(III) ions
- Application of microprecipitation on membrane filters for Alpha Spectrometry, liquid scintillation counting and Mass Spectrometric determination of plutonium isotopes
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- Input contribution and vertical migration of plutonium, americium and cesium in lake sediments (Belham Tarn, Cumbria, UK)
- Diffusion of 22Na+, 85Sr2+, 134Cs+ and 57Co2+ in bentonite clay compacted to different densities: experiments and modeling
- U6+ phases in the weathering zone of the Bangombé U-deposit: observed and predicted mineralogy
- Precipitation of technetium by subsurface sulfate-reducing bacteria
- Spectroscopic studies on the interaction of U(VI) with Bacillus sphaericus
- Bio-sorption of neptunium(V) by Pseudomonas fluorescens
- Biotransformation of pertechnetate by Clostridia
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