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Biotransformation of pertechnetate by Clostridia

  • Arokiasamy J. Francis , Cleveland J. Dodge and G. E. Meinken
Published/Copyright: September 25, 2009
Radiochimica Acta
From the journal Radiochimica Acta Volume 90 Issue 9-11

Summary

Clostridia are strict anaerobic, spore-forming, fermentative bacteria commonly present in soils, sediments, and wastes; and, they play a major role in the decomposition of a wide variety of organic compounds. They also are involved in the reduction of iron, manganese, and uranium, thereby affecting their solubility. However, little is known of the ability of Clostridia to reduce technetium (Tc). We investigated the reduction and precipitation of pertechnetate by Clostridium sphenoides able to metabolize citrate as its sole carbon source, and Clostridium sp. capable of fermenting glucose but not citric acid. Both species reduced Tc(VII) to Tc(IV), although C. sphenoides did so at a greater rate and extent than Clostridium sp. The reduced Tc was predominantly associated with the cell biomass. It also was present in solution complexed with bacterial metabolic products (MW>5000). Adding diethylenetriaminepentaacetic acid (DTPA) to Clostridium sp. resulted in the formation of a soluble Tc(IV)-DTPA complex, whereas with C. sphenoides only a small amount of Tc was present in solution, indicating that insoluble Tc species were formed. These results suggest that Clostridia may play a major role in regulating the mobility of Tc under anaerobic conditions in wastes and subsurface environments.

Received: 2001-9-4
Accepted: 2002-4-11
Published Online: 2009-9-25
Published in Print: 2002-9-1

© Oldenbourg Wissenschaftsverlag, München

Articles in the same Issue

  1. Solubility of amorphous Th(IV) hydroxide – application of LIBD to determine the solubility product and EXAFS for aqueous speciation
  2. Thermodynamics of the U(VI)-Ca2+-Cl −-OH −-H2O system: Solubility product of becquerelite
  3. Control of plutonium dioxide solubility by amorphous tetrahydroxide: A critical review of the model
  4. Technetium migration in fluoroapatite during thermal annealing
  5. Uranium release from boom clay in bicarbonate media
  6. Coprecipitation of thorium with UO2
  7. Formation of secondary phases after long-term corrosion of simulated HLW glass in brine solutions at 190 °C
  8. Sorption behaviour of Am on precorroded HLW glass in water and brines
  9. Molecular fluorescence spectroscopy and mixture analysis for the evaluation of the complexation between humic acid and UO22+
  10. Investigation on uranyl interaction with bioactive ligands. Synthesis and structural studies of the uranyl complexes with glycine and N-(2-mercaptopropionyl)glycine
  11. Complex formation of calcium with humic acid and polyacrylic acid
  12. Complexation of americium with humic, fulvic and citric acids at high ionic strength
  13. Complex formation of Eu(III) with humic acid and polyacrylic acid
  14. XAS study of technetium(IV) polymer formation in mixed sulphate/chloride media
  15. Complexation of uranium(VI) with malonate at variable temperatures
  16. The hydrolysis of uranium(VI)
  17. Corrosion and dissolution studies of UO2 containing α-emitters
  18. The effects of alpha-radiolysis on UO2 dissolution determined from electrochemical experiments with 238Pu-doped UO2
  19. Radiolytic formation of Tc(IV) oxide colloids
  20. Radiation-chemical effects in the near-field of a final disposal site: role of bromine on the radiolytic processes in NaCl-solutions
  21. The reduction of U(VI) on corroded iron under anoxic conditions
  22. New selenium solution speciation method by ion chromatography + gamma counting and its application to FeS2-controlled reducing conditions
  23. Uptake of oxo-anions by cements through solid-solution formation: experimental evidence and modelling
  24. Iodine species uptake by cement and CSH studied by I K-edge X-ray absorption spectroscopy
  25. Structure of uranium sorption complexes at montmorillonite edge sites
  26. Sorption of several safety relevant radionuclides on granite and diorite – a potential repository host rock in the Taiwan area
  27. Sorption and reduction of neptunium(V) on the surface of iron oxides
  28. Uptake mechanism of IO3− to NO3−-hydrotalcite
  29. Structure alteration of C-S-H (calcium silicate hydrated phases) caused by sorption of caesium
  30. The effect of α-isosaccharinic acid on the stability of and Th(IV) uptake by hardened cement paste
  31. Complexation studies of Eu(III) with polyacrylic acid either free in solution or adsorbed onto alumina
  32. Sorption behaviour of caesium on a bentonite sample
  33. Specific interaction of cesium with the surface of calcium silicate hydrates
  34. Relationship of quantitative X-ray diffraction measurements of geologic materials to cesium sorption
  35. Instrumental development for spectroscopic speciation of f-elements in hydrothermal solutions: luminescence properties of lanthanide(III) ions
  36. Application of microprecipitation on membrane filters for Alpha Spectrometry, liquid scintillation counting and Mass Spectrometric determination of plutonium isotopes
  37. Characterization of aquatic colloids by a combination of LIBD and ICP-MS following the size fractionation
  38. Technetium behaviour in Boom Clay – a laboratory and field study
  39. The use of electromigration as a qualitative technique to study the migration behaviour and speciation of uranium in the Boom Clay
  40. Input contribution and vertical migration of plutonium, americium and cesium in lake sediments (Belham Tarn, Cumbria, UK)
  41. Diffusion of 22Na+, 85Sr2+, 134Cs+ and 57Co2+ in bentonite clay compacted to different densities: experiments and modeling
  42. U6+ phases in the weathering zone of the Bangombé U-deposit: observed and predicted mineralogy
  43. Precipitation of technetium by subsurface sulfate-reducing bacteria
  44. Spectroscopic studies on the interaction of U(VI) with Bacillus sphaericus
  45. Bio-sorption of neptunium(V) by Pseudomonas fluorescens
  46. Biotransformation of pertechnetate by Clostridia
  47. Solubility and hydrolysis of Zr oxides: a review and supplemental data
  48. Nagra/PSI Chemical Thermodynamic Data Base 01/01
  49. Sorption modelling by Gibbs energy minimisation: Towards a uniform thermodynamic database for surface complexes of radionuclides
https://doi.org/10.1524/ract.2002.90.9-11_2002.791

Articles in the same Issue

  1. Solubility of amorphous Th(IV) hydroxide – application of LIBD to determine the solubility product and EXAFS for aqueous speciation
  2. Thermodynamics of the U(VI)-Ca2+-Cl −-OH −-H2O system: Solubility product of becquerelite
  3. Control of plutonium dioxide solubility by amorphous tetrahydroxide: A critical review of the model
  4. Technetium migration in fluoroapatite during thermal annealing
  5. Uranium release from boom clay in bicarbonate media
  6. Coprecipitation of thorium with UO2
  7. Formation of secondary phases after long-term corrosion of simulated HLW glass in brine solutions at 190 °C
  8. Sorption behaviour of Am on precorroded HLW glass in water and brines
  9. Molecular fluorescence spectroscopy and mixture analysis for the evaluation of the complexation between humic acid and UO22+
  10. Investigation on uranyl interaction with bioactive ligands. Synthesis and structural studies of the uranyl complexes with glycine and N-(2-mercaptopropionyl)glycine
  11. Complex formation of calcium with humic acid and polyacrylic acid
  12. Complexation of americium with humic, fulvic and citric acids at high ionic strength
  13. Complex formation of Eu(III) with humic acid and polyacrylic acid
  14. XAS study of technetium(IV) polymer formation in mixed sulphate/chloride media
  15. Complexation of uranium(VI) with malonate at variable temperatures
  16. The hydrolysis of uranium(VI)
  17. Corrosion and dissolution studies of UO2 containing α-emitters
  18. The effects of alpha-radiolysis on UO2 dissolution determined from electrochemical experiments with 238Pu-doped UO2
  19. Radiolytic formation of Tc(IV) oxide colloids
  20. Radiation-chemical effects in the near-field of a final disposal site: role of bromine on the radiolytic processes in NaCl-solutions
  21. The reduction of U(VI) on corroded iron under anoxic conditions
  22. New selenium solution speciation method by ion chromatography + gamma counting and its application to FeS2-controlled reducing conditions
  23. Uptake of oxo-anions by cements through solid-solution formation: experimental evidence and modelling
  24. Iodine species uptake by cement and CSH studied by I K-edge X-ray absorption spectroscopy
  25. Structure of uranium sorption complexes at montmorillonite edge sites
  26. Sorption of several safety relevant radionuclides on granite and diorite – a potential repository host rock in the Taiwan area
  27. Sorption and reduction of neptunium(V) on the surface of iron oxides
  28. Uptake mechanism of IO3− to NO3−-hydrotalcite
  29. Structure alteration of C-S-H (calcium silicate hydrated phases) caused by sorption of caesium
  30. The effect of α-isosaccharinic acid on the stability of and Th(IV) uptake by hardened cement paste
  31. Complexation studies of Eu(III) with polyacrylic acid either free in solution or adsorbed onto alumina
  32. Sorption behaviour of caesium on a bentonite sample
  33. Specific interaction of cesium with the surface of calcium silicate hydrates
  34. Relationship of quantitative X-ray diffraction measurements of geologic materials to cesium sorption
  35. Instrumental development for spectroscopic speciation of f-elements in hydrothermal solutions: luminescence properties of lanthanide(III) ions
  36. Application of microprecipitation on membrane filters for Alpha Spectrometry, liquid scintillation counting and Mass Spectrometric determination of plutonium isotopes
  37. Characterization of aquatic colloids by a combination of LIBD and ICP-MS following the size fractionation
  38. Technetium behaviour in Boom Clay – a laboratory and field study
  39. The use of electromigration as a qualitative technique to study the migration behaviour and speciation of uranium in the Boom Clay
  40. Input contribution and vertical migration of plutonium, americium and cesium in lake sediments (Belham Tarn, Cumbria, UK)
  41. Diffusion of 22Na+, 85Sr2+, 134Cs+ and 57Co2+ in bentonite clay compacted to different densities: experiments and modeling
  42. U6+ phases in the weathering zone of the Bangombé U-deposit: observed and predicted mineralogy
  43. Precipitation of technetium by subsurface sulfate-reducing bacteria
  44. Spectroscopic studies on the interaction of U(VI) with Bacillus sphaericus
  45. Bio-sorption of neptunium(V) by Pseudomonas fluorescens
  46. Biotransformation of pertechnetate by Clostridia
  47. Solubility and hydrolysis of Zr oxides: a review and supplemental data
  48. Nagra/PSI Chemical Thermodynamic Data Base 01/01
  49. Sorption modelling by Gibbs energy minimisation: Towards a uniform thermodynamic database for surface complexes of radionuclides
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