Startseite Effect of Dehydrated Trehalose Matrix on the Kinetics of Forward Electron Transfer Reactions in Photosystem I
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Effect of Dehydrated Trehalose Matrix on the Kinetics of Forward Electron Transfer Reactions in Photosystem I

  • Ivan Shelaev , Michael Gorka , Anton Savitsky EMAIL logo , Vasily Kurashov , Mahir Mamedov , Fedor Gostev , Klaus Möbius EMAIL logo , Victor Nadtochenko EMAIL logo , John Golbeck EMAIL logo und Alexey Semenov EMAIL logo
Veröffentlicht/Copyright: 3. November 2016
Zeitschrift für Physikalische Chemie
Aus der Zeitschrift Zeitschrift für Physikalische Chemie Band 231 Heft 2

Abstract

The effect of dehydration on the kinetics of forward electron transfer (ET) has been studied in cyanobacterial photosystem I (PS I) complexes in a trehalose glassy matrix by time-resolved optical and EPR spectroscopies in the 100 fs to 1 ms time domain. The kinetics of the flash-induced absorption changes in the subnanosecond time domain due to primary and secondary charge separation steps were monitored by pump–probe laser spectroscopy with 20-fs low-energy pump pulses centered at 720 nm. The back-reaction kinetics of P700 were measured by high-field time-resolved EPR spectroscopy and the forward kinetics of A1A•−/A1B•−→FX by time-resolved optical spectroscopy at 480 nm. The kinetics of the primary ET reactions to form the primary P700•+A0•− and the secondary P700•+A1•− ion radical pairs were not affected by dehydration in the trehalose matrix, while the yield of the P700•+A1•− was decreased by ~20%. Forward ET from the phylloquinone molecules in the A1A•− and A1B•− sites to the iron–sulfur cluster FX slowed from ~220 ns and ~20 ns in solution to ~13 μs and ~80 ns, respectively. However, as shown by EPR spectroscopy, the ~15 μs kinetic phase also contains a small contribution from the recombination between A1B•− and P700•+. These data reveal that the initial ET reactions from P700 to secondary phylloquinone acceptors in the A- and B-branches of cofactors (A1A and A1B) remain unaffected whereas ET beyond A1A and A1B is slowed or prevented by constrained protein dynamics due to the dry trehalose glass matrix.

Keywords: electron transfer; EPR; optical spectroscopy; photosystem I; trehalose matrix

Dedicated to: Kev Salikhov on the occasion of his 80th birthday.

Funding source: Russian Foundation for Basic Research

Award Identifier / Grant number: 15-04-04252

Funding source: Russian Science Foundation

Award Identifier / Grant number: 14-14-00789

Funding statement: This work was financially supported by the Russian Foundation for Basic Research, grant 15-04-04252 (to A.Yu.S.) and Russian Science Foundation, grant 14-14-00789 (to A.Yu.S. and V.N.), by the President of RF grant MK-6515.2015.4 (to I.S.), by the US National Science Foundation, grant MCB-1613022 (to J.G.) and by the Cluster of Excellence RESOLV (EXC 1069) funded by the Deutsche Forschungsgemeinschaft (to A.S.) and the Max Planck Society (to A.S. and K.M.). K.M. acknowledges sustaining support by the Free University of Berlin. We want to thank Giovanni Venturoli (University of Bologna) for stimulating and fruitful discussions, Dmitry Cherepanov and Georgy Milanovsky for their assistance in application of CONTIN software, and Anastasia Petrova for preparation of PS I complexes (all Moscow State University).

Acknowledgments

This work was financially supported by the Russian Foundation for Basic Research, grant 15-04-04252 (to A.Yu.S.) and Russian Science Foundation, grant 14-14-00789 (to A.Yu.S. and V.N.), by the President of RF grant MK-6515.2015.4 (to I.S.), by the US National Science Foundation, grant MCB-1613022 (to J.G.) and by the Cluster of Excellence RESOLV (EXC 1069) funded by the Deutsche Forschungsgemeinschaft (to A.S.) and the Max Planck Society (to A.S. and K.M.). K.M. acknowledges sustaining support by the Free University of Berlin. We want to thank Giovanni Venturoli (University of Bologna) for stimulating and fruitful discussions, Dmitry Cherepanov and Georgy Milanovsky for their assistance in application of CONTIN software, and Anastasia Petrova for preparation of PS I complexes (all Moscow State University).

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Received: 2016-7-18
Accepted: 2016-10-5
Published Online: 2016-11-3
Published in Print: 2017-2-1

©2017 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Preface
  3. Editorial Review
  4. From Free Radicals and Spin-Chemistry Over Spin-Dynamics and Hyperpolarization to Biology and Materials Science
  5. On Relations Between Singlet and Triplet Recombination Yields for Singlet and Triplet Precursors
  6. The Quantum Dynamical Basis of a Classical Kinetic Scheme Describing Coherent and Incoherent Regimes of Radical Pair Recombination
  7. Reaction Operators for Radical Pairs. The Exactly Solvable Model
  8. Estimation of the Fraction of Spin-Correlated Radical Ion Pairs in Irradiated Alkanes using Magnetosensitive Recombination Luminescence from Exciplexes Generated upon Recombination of a Probe Pair
  9. Theoretical Description of Pulsed RYDMR: Refocusing Zero-Quantum and Single Quantum Coherences
  10. A Transient EPR Study of Electron Transfer in Tetrathiafulvalene-Aluminum(III) Porphyrin-Anthraquinone Supramolecular Triads
  11. Solvent Radical Anions in Irradiated Aliphatic Ketones and Esters as Observed Using Time-Resolved Magnetic Field Effects in the Recombination Fluorescence
  12. Effect of Dehydrated Trehalose Matrix on the Kinetics of Forward Electron Transfer Reactions in Photosystem I
  13. Photo-CIDNP in the Reaction Center of the Diatom Cyclotella meneghiniana Observed by 13C MAS NMR
  14. Light-Stimulated Generation of Free Radicals by Quinones-Chelators
  15. Influence of C2-Methylation of Imidazolium Based Ionic Liquids on Photoinduced Spin Dynamics of the Dissolved ZnTPP Studied by Time-Resolved EPR
  16. ESR Study of Electron States in Ge/Si Heterostructures with Nanodisc Shaped Quantum Dots
  17. Novel Anthrathiophene-Based Small Molecules as Donor Material for Organic Photovoltaics: Synthesis and Light-Induced EPR Study
  18. Structural Determination of a DNA Oligomer for a Molecular Spin Qubit Lloyd Model of Quantum Computers
https://doi.org/10.1515/zpch-2016-0860
Schlagwörter für diesen Artikel
electron transfer; EPR; optical spectroscopy; photosystem I; trehalose matrix

Artikel in diesem Heft

  1. Frontmatter
  2. Preface
  3. Editorial Review
  4. From Free Radicals and Spin-Chemistry Over Spin-Dynamics and Hyperpolarization to Biology and Materials Science
  5. On Relations Between Singlet and Triplet Recombination Yields for Singlet and Triplet Precursors
  6. The Quantum Dynamical Basis of a Classical Kinetic Scheme Describing Coherent and Incoherent Regimes of Radical Pair Recombination
  7. Reaction Operators for Radical Pairs. The Exactly Solvable Model
  8. Estimation of the Fraction of Spin-Correlated Radical Ion Pairs in Irradiated Alkanes using Magnetosensitive Recombination Luminescence from Exciplexes Generated upon Recombination of a Probe Pair
  9. Theoretical Description of Pulsed RYDMR: Refocusing Zero-Quantum and Single Quantum Coherences
  10. A Transient EPR Study of Electron Transfer in Tetrathiafulvalene-Aluminum(III) Porphyrin-Anthraquinone Supramolecular Triads
  11. Solvent Radical Anions in Irradiated Aliphatic Ketones and Esters as Observed Using Time-Resolved Magnetic Field Effects in the Recombination Fluorescence
  12. Effect of Dehydrated Trehalose Matrix on the Kinetics of Forward Electron Transfer Reactions in Photosystem I
  13. Photo-CIDNP in the Reaction Center of the Diatom Cyclotella meneghiniana Observed by 13C MAS NMR
  14. Light-Stimulated Generation of Free Radicals by Quinones-Chelators
  15. Influence of C2-Methylation of Imidazolium Based Ionic Liquids on Photoinduced Spin Dynamics of the Dissolved ZnTPP Studied by Time-Resolved EPR
  16. ESR Study of Electron States in Ge/Si Heterostructures with Nanodisc Shaped Quantum Dots
  17. Novel Anthrathiophene-Based Small Molecules as Donor Material for Organic Photovoltaics: Synthesis and Light-Induced EPR Study
  18. Structural Determination of a DNA Oligomer for a Molecular Spin Qubit Lloyd Model of Quantum Computers
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