Poly-acrylic Acid Brushes and Adsorbed Proteins
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Matthias Reinhardt
Abstract
Planar polyelectrolyte brushes are prepared by Langmuir–Schaefer based grafting of perdeuterated (styrene)49-b-(acrylic acid)222 block copolymer (dPS-b-PAA) to dPS pre-coated silicon supports with grafting density σPAA from 0.07 to 0.11 nm–2. The structure of the solvent-swollen brushes, i. e. the volume fraction profile of polymer segments, ϕPAA, as a function of altitude z from the grafting plane into the liquid phase is extracted from neutron reflectivity measurements. We find that for all cases investigated ϕPAA(z) resembles a Gaussian profile. Although very condensed, the PAA brushes can be loaded with bovine serum albumin (BSA). The integral amount of adsorbed BSA scales linearly with grafting density. We compare our z-resolved volume fraction profile ϕBSA(z) of BSA on PAA brushes with existing literature on that system. It is found that a cross-over takes place in the adsorption scheme from ternary compressive, where proteins can approach the grafting surface only by compressing the brush, to ternary insertive, where proteins enter the brush with only local perturbation of the concentration profile, as a function of RP/Hmax, where RP is the Stokes-Radius of the protein, and Hmax is the experimentally determined maximum height of the brush.
Acknowledgement
This work is based on experiments performed at the Swiss spallation neutron source SINQ, Paul Scherrer Institut (PSI), Villigen, Switzerland. The authors thank Helmholtz-Zentrum Berlin and Paul Scherrer Institut for financial support.
©2015 Walter de Gruyter Berlin/Boston
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Articles in the same Issue
- Frontmatter
- Preface
- Gerhard Findenegg: A Scientific Life in Soft Matter at Interfaces
- Nanoparticles via Oil-in-Water Microemulsions: a Solvent-Reduced, Energy-Efficient Approach
- Formation of Anisometric Fumed Silica Supraparticles – Mechanism and Application Potential
- Multidirectional, Multicomponent Electric Field Driven Assembly of Complex Colloidal Chains
- Polymer Brush/Metal Nanoparticle Hybrids for Optical Sensor Applications: from Self-Assembly to Tailored Functions and Nanoengineering
- Poly-acrylic Acid Brushes and Adsorbed Proteins
- Interactions of Two Fragments of the Human Antimicrobial Peptide LL-37 with Zwitterionic and Anionic Lipid Monolayers
- Depletion Interaction Mediated by fd-Virus: on the Limit of Low Density and Derjaguin Approximation
- Transport Properties of Polyelectrolyte Solutions. Effect of Confinement in Thin Liquid Films
- Relationship Between Pore Structure and Sorption-Induced Deformation in Hierarchical Silica-Based Monoliths
- Ammonia Dissociation on Graphene Oxide: An Ab Initio Density Functional Theory Calculation
- Responsive Microgels at Surfaces and Interfaces
- Buckled Topography to Enhance Light Absorption in Thin Film Organic Photovoltaics Comprising CuPc/C60 Bilayer Laminates
