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Determination of essential oil and phenolic compounds of Berberis vulgaris grown in Şavşat, Artvin; revealing its antioxidant and antimicrobial activities

  • Hatice Iskender ORCID logo EMAIL logo , Sule Ceylan , Eda Dokumacioglu , Aysegul Saral Sariyer , Ali Karagoz and Hayal Akyildirim Begen
Published/Copyright: December 31, 2024
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Abstract

This study is to determine the antioxidant capacities, fatty acids and phenolic compounds, and antimicrobial activity in Berberis vulgaris. The antioxidant contents of the plants were determined using the CUPRAC, FRAP, and DPPH methods. The total phenolic content was determined using the Folin–Ciocalteu method, the phenolic compound profile was analyzed by HPLC, and the antimicrobial activity was assessed using the liquid microdilution method. The total polyphenol content was found to be 27.65 ± 1.12 mg GAE/g sample in the fruit of the plant with the highest amount, while the total flavonoid content was 15.13 ± 0.92 mg quercetin/g sample in the leaf part of the plant with the highest amount. Chlorogenic acid was identified as the major component in the fruit and leaf of B. vulgaris with concentrations of 1,195.56 ppm and 1,113.46 ppm, respectively. Catechin was identified as the major component in the seed part with a concentration of 130.29 ppm. B. vulgaris is a valuable source of bioactive phytochemicals with high functional quality.


Corresponding author: Hatice Iskender, Department of Nutrition and Dietetics, Faculty of Healthy Sciences, Artvin Coruh University, Artvin, 08000, Türkiye, E-mail:

  1. Research ethics: Not applicable.

  2. Informed consent: Not applicable.

  3. Author contributions: IH and KA designed the experiments; CS, IH, and DE analyzed and interpreted the data. SSA and CS performed the experiments. ABH and CS prepared and characterized the extract. All authors read and approved the final manuscript.

  4. Use of Large Language Models, AI and Machine Learning Tools: None declared.

  5. Conflict of interests: The authors show no conflict of interests.

  6. Research funding: None declared.

  7. Data availability: The raw data can be obtained on request from the corresponding author.

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Received: 2024-07-13
Accepted: 2024-12-13
Published Online: 2024-12-31
Published in Print: 2025-07-28

© 2024 Walter de Gruyter GmbH, Berlin/Boston

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