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Coenzyme Q10 supplementation affects cellular ionic balance: relevance to aging

  • Parisha Srivastava , Sukanya Bhoumik , Arun K. Yadawa , Rashmi Kesherwani and Syed Ibrahim Rizvi ORCID logo EMAIL logo
Published/Copyright: July 5, 2024
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Zeitschrift für Naturforschung C
From the journal Zeitschrift für Naturforschung C Volume 80 Issue 3-4

Abstract

Aging results into disruptive physiological functioning and cellular processes that affect the composition and structure of the plasma membrane. The plasma membrane is the major regulator of ionic homeostasis that regulates the functioning of membrane transporters and exchangers. Coenzyme Q10 is a lipid-soluble antioxidant molecule that declines during aging and age-associated diseases. The present study aims to explore the role of Coenzyme Q10 supplementation to rats during aging on membrane transporters and redox biomarkers. The study was conducted on young and old male Wistar rats supplemented with 20 mg/kg b.w. of Coenzyme Q10 per day. After a period of 28 days, rats were sacrificed and erythrocyte membrane was isolated. The result exhibits significant decline in biomarkers of oxidative stress in old control rats when compared with young control. The effect of Coenzyme Q10 supplementation was more pronounced in old rats. The functioning of membrane transporters and Na+/H+ exchanger showed potential return to normal levels in the Coenzyme Q10 treated rats. Overall, the results demonstrate that Coenzyme Q10 plays an important role in maintaining redox balance in cells which interconnects with membrane integrity. Thus, Coenzyme Q10 supplementation may play an important role in protecting age related alterations in erythrocyte membrane physiology.

Keywords: aging; Coenzyme Q10; ionic balance; membrane transporters; oxidative stress
Corresponding author: Syed Ibrahim Rizvi, Department of Biochemistry, University of Allahabad, Allahabad, Uttar Pradesh 211002, India, E-mail:

Acknowledgments

Authors gratefully acknowledge for the funding and facilities received by Department of Biochemistry, University of Allahabad, India and Department of Biotechonology, New Delhi.

  1. Research ethics: The protocol of the study was approved by the Animal Ethical Committee of University of Allahabad.

  2. Author contributions: PS and SIR conceived and designed the experiments. SB, AKY and RK performed the experiments. PS and SIR analyzed the data and wrote the paper. The authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: The authors declare no conflicts of interest regarding this article.

  4. Research funding: The research is funded by UGC-SAP grant of Department of Biochemistry, University of Allahabad, India and Department of Biotechnology, Govt of India.

  5. Data availability: Data generated or analyzed during this study is available online at https://doi.org/10.17632/xcjc3wpzkr.1.

References

1. da Costa, JP, Vitorino, R, Silva, GM, Vogel, C, Duarte, AC, Rocha-Santos, T. A synopsis on aging-theories, mechanisms and future prospects. Ageing Res Rev 2016;29:90–112. https://doi.org/10.1016/j.arr.2016.06.005.Search in Google Scholar PubMed PubMed Central

2. Warraich, U-A, Hussain, F, Kayani, HUR. Aging – oxidative stress, antioxidants and computational modeling. Heliyon 2020;6:e04107. https://doi.org/10.1016/j.heliyon.2020.e04107.Search in Google Scholar PubMed PubMed Central

3. Guo, J, Huang, X, Dou, L, Yan, M, Shen, T, Tang, W, et al.. Aging and aging-related diseases: from molecular mechanisms to interventions and treatments. Signal Transduct Targeted Ther 2022;7:1–40. https://doi.org/10.1038/s41392-022-01251-0.Search in Google Scholar PubMed PubMed Central

4. Cho, I, Jackson, MR, Swift, J. Roles of cross-membrane transport and signaling in the maintenance of cellular homeostasis. Cell Mol Bioeng 2016;9:234–46. https://doi.org/10.1007/s12195-016-0439-6.Search in Google Scholar PubMed PubMed Central

5. Ward, PS, Thompson, CB. Signaling in control of cell growth and metabolism. Cold Spring Harbor Perspect Biol 2012;4:a006783. https://doi.org/10.1101/cshperspect.a006783.Search in Google Scholar PubMed PubMed Central

6. Lombard, J. Once upon a time the cell membranes: 175 years of cell boundary research. Biol Direct 2014;9:32. https://doi.org/10.1186/s13062-014-0032-7.Search in Google Scholar PubMed PubMed Central

7. Pizzagalli, MD, Bensimon, A, Superti-Furga, G. A guide to plasma membrane solute carrier proteins. FEBS J 2021;288:2784–835. https://doi.org/10.1111/febs.15531.Search in Google Scholar PubMed PubMed Central

8. Pallotti, F, Bergamini, C, Lamperti, C, Fato, R. The roles of coenzyme Q in disease: direct and indirect involvement in cellular functions. Int J Mol Sci 2021;23:128. https://doi.org/10.3390/ijms23010128.Search in Google Scholar PubMed PubMed Central

9. Hernández-Camacho, JD, Bernier, M, López-Lluch, G, Navas, P. Coenzyme Q10 supplementation in aging and disease. Front Physiol 2018;9. https://doi.org/10.3389/fphys.2018.00044.Search in Google Scholar PubMed PubMed Central

10. Aaseth, J, Alexander, J, Alehagen, U. Coenzyme Q10 supplementation – in ageing and disease. Mech Ageing Dev 2021;197:111521. https://doi.org/10.1016/j.mad.2021.111521.Search in Google Scholar PubMed

11. Srivastava, P, Verma, AK, Arya, JK, Rizvi, SI. Modulatory effect of exogenous Coenzyme Q10 on redox and inflammatory biomarkers during aging in rats. Biol Futura 2022;73:473–81. https://doi.org/10.1007/s42977-022-00140-5.Search in Google Scholar PubMed

12. Luo, K, Yu, JH, Quan, Y, Shin, YJ, Lee, KE, Kim, HL, et al.. Therapeutic potential of Coenzyme Q10 in mitochondrial dysfunction during tacrolimus-induced beta cell injury. Sci Rep 2019;9:7995. https://doi.org/10.1038/s41598-019-44475-x.Search in Google Scholar PubMed PubMed Central

13. Kumar, VA, Singh, S, Srivastava, P, Ibrahim Rizvi, S. Melatonin stabilizes age-dependent alterations in erythrocyte membrane induced by ‘artificial light at night’ in a chronodisrupted model of rat. Gen Comp Endocrinol 2022;316:113960. https://doi.org/10.1016/j.ygcen.2021.113960.Search in Google Scholar PubMed

14. Spyridaki, M-HE, Siskos, PA. An improved spectrophotometric method for the determination of free, bound and total N-acetylneuraminic acid in biological fluids. Anal Chim Acta 1996;327:277–85. https://doi.org/10.1016/0003-2670(96)00073-6.Search in Google Scholar

15. Kitajima, H, Yamaguchi, T, Kimoto, E. Hemolysis of human erythrocytes under hydrostatic pressure is suppressed by cross-linking of membrane proteins. J Biochem 1990;108:1057–62. https://doi.org/10.1093/oxfordjournals.jbchem.a123305.Search in Google Scholar PubMed

16. Firuzi, O, Mladenka, P, Riccieri, V, Spadaro, A, Petrucci, R, Marrosu, G, et al.. Parameters of oxidative stress status in healthy subjects: their correlations and stability after sample collection. J Clin Lab Anal 2006;20:139–48. https://doi.org/10.1002/jcla.20122.Search in Google Scholar PubMed PubMed Central

17. Singh, S, Pandey, KB, Rizvi, SI. Erythrocyte senescence and membrane transporters in young and old rats. Arch Physiol Biochem 2016;122:228–34. https://doi.org/10.1080/13813455.2016.1190761.Search in Google Scholar PubMed

18. Lowry, OH, Rosebrough, NJ, Farr, AL, Randall, R. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265–75. https://doi.org/10.1016/s0021-9258(19)52451-6.Search in Google Scholar

19. Arya, JK, Kumar, R, Singh, A, Srivastava, P, Yadawa, AK, Rizvi, SI. Acarbose, an α-glucosidase inhibitor, maintains altered redox homeostasis during aging by targeting glucose metabolism in rat erythrocytes. Rejuvenation Res 2023;26:21–31. https://doi.org/10.1089/rej.2022.0032.Search in Google Scholar PubMed

20. Fiske, CH, Subbarow, Y. The colorimetric determination of phosphorus. J Biol Chem 1925;66:375–400. https://doi.org/10.1016/s0021-9258(18)84756-1.Search in Google Scholar

21. Zaidi, A. Plasma membrane Ca2+-ATPases: targets of oxidative stress in brain aging and neurodegeneration. World J Biol Chem 2010;1:271–80. https://doi.org/10.4331/wjbc.v1.i9.271.Search in Google Scholar PubMed PubMed Central

22. Pandey, KB, Rizvi, SI. Role of resveratrol in regulation of membrane transporters and integrity of human erythrocytes. Biochem Biophys Res Commun 2014;453:521–6. https://doi.org/10.1016/j.bbrc.2014.09.117.Search in Google Scholar PubMed

23. Verma, AK, Garg, G, Singh, S, Rizvi, SI. Melatonin protects against membrane alterations affected by ‘artificial light at night’ in a circadian-disrupted model of rat. Biol Rhythm Res 2022;53:317–28. https://doi.org/10.1080/09291016.2020.1741265.Search in Google Scholar

24. Antonelou, MH, Kriebardis, AG, Papassideri, IS. Aging and death signalling in mature red cells: from basic science to transfusion practice. Blood Transfus 2010;8:s39. https://doi.org/10.2450/2010.007S.Search in Google Scholar PubMed PubMed Central

25. Massaccesi, L, Galliera, E, Corsi Romanelli, MM. Erythrocytes as markers of oxidative stress related pathologies. Mech Ageing Dev 2020;191:111333. https://doi.org/10.1016/j.mad.2020.111333.Search in Google Scholar PubMed

26. Singh, S, Garg, G, Singh, AK, Tripathi, SS, Rizvi, SI. Fisetin, a potential caloric restriction mimetic, modulates ionic homeostasis in senescence induced and naturally aged rats. Arch Physiol Biochem 2022;128:51–8. https://doi.org/10.1080/13813455.2019.1662452.Search in Google Scholar PubMed

27. Zhao, Y, Hu, X, Liu, Y, Dong, S, Wen, Z, He, W, et al.. ROS signaling under metabolic stress: cross-talk between AMPK and AKT pathway. Mol Cancer 2017;16:79. https://doi.org/10.1186/s12943-017-0648-1.Search in Google Scholar PubMed PubMed Central

28. Qin, X, Li, H, Zhao, H, Fang, L, Wang, X. Enhancing healthy aging with small molecules: a mitochondrial perspective. Med Res Rev 2024;44:1904–22. https://doi.org/10.1002/med.22034.Search in Google Scholar PubMed

29. Gasmi, A, Bjørklund, G, Mujawdiya, PK, Semenova, Y, Piscopo, S, Peana, M. Coenzyme Q10 in aging and disease. Crit Rev Food Sci Nutr 2024;64:3907–19. https://doi.org/10.1080/10408398.2022.2137724.Search in Google Scholar PubMed

30. Silva, SVE, Gallia, MC, Luz, JRD da, Rezende, AAd, Bongiovanni, GA, Araujo-Silva, G, et al.. Antioxidant effect of coenzyme Q10 in the prevention of oxidative stress in arsenic-treated CHO-K1 cells and possible participation of zinc as a pro-oxidant agent. Nutrients 2022;14:3265. https://doi.org/10.3390/nu14163265.Search in Google Scholar PubMed PubMed Central

31. Liu, J-K. Antiaging agents: safe interventions to slow aging and healthy life span extension. Nat Prod Bioprospect 2022;12:18. https://doi.org/10.1007/s13659-022-00339-y.Search in Google Scholar PubMed PubMed Central

32. Graczyk, P, Dach, A, Dyrka, K, Pawlik, A. Pathophysiology and advances in the therapy of cardiomyopathy in patients with diabetes mellitus. Int J Mol Sci 2024;25:5027. https://doi.org/10.3390/ijms25095027.Search in Google Scholar PubMed PubMed Central

33. Liao, M, He, X, Zhou, Y, Peng, W, Zhao, XM, Jiang, M. Coenzyme Q10 in atherosclerosis. Eur J Pharmacol 2024;970:176481. https://doi.org/10.1016/j.ejphar.2024.176481.Search in Google Scholar PubMed

34. Liaghat, M, Yaghoubzad-Maleki, M, Nabi-Afjadi, M, Fathi, Z, Zalpoor, H, Heidari, N, et al.. A review of the potential role of CoQ10 in the treatment of hepatocellular carcinoma. Biochem Genet 2024;62:575–93. https://doi.org/10.1007/s10528-023-10490-x.Search in Google Scholar PubMed

35. Ghosh, S. Sialic acid and biology of life: an introduction. In: Sialic acids and sialoglycoconjugates in the biology of life, health and disease. Amsterdam, Netherlands: Elsevier Science; 2020:1–61 pp.10.1016/B978-0-12-816126-5.00001-9Search in Google Scholar

36. Cho, A, Christine, M, Malicdan, V, Miyakawa, M, Nonaka, I, Nishino, I, et al.. Sialic acid deficiency is associated with oxidative stress leading to muscle atrophy and weakness in GNE myopathy. Hum Mol Genet 2017;26:3081–93. https://doi.org/10.1093/hmg/ddx192.Search in Google Scholar PubMed PubMed Central

37. Shahvali, S, Shahesmaeili, A, Sanjari, M, Karami-Mohajeri, S. The correlation between blood oxidative stress and sialic acid content in diabetic patients with nephropathy, hypertension, and hyperlipidemia. Diabetol Int 2019;11:19–26. https://doi.org/10.1007/s13340-019-00395-9.Search in Google Scholar PubMed PubMed Central

38. Solakhan, M, Çiçek, H, Orhan, N, Yildirim, M. Role of native thiol, total thiol and dynamic disulphide in diagnosis of patient with prostate cancer and prostatitis. Int Braz J Urol 2019;45:495–502. https://doi.org/10.1590/s1677-5538.ibju.2018.0469.Search in Google Scholar

39. Samimi, F, Baazm, M, Eftekhar, E, Rajabi, S, Goodarzi, M. Possible antioxidant mechanism of Coenzyme Q10 in diabetes: impact on Sirt1/Nrf2 signaling pathways. Res Pharm Sci 2019;14:524–33. https://doi.org/10.4103/1735-5362.272561.Search in Google Scholar PubMed PubMed Central

40. Bogdanova, A, Petrushanko, IY, Hernansanz-Agustín, P, Martínez-Ruiz, A. “Oxygen sensing” by Na, K-ATPase: these miraculous thiols. Front Physiol 2016;7:314. https://doi.org/10.3389/fphys.2016.00314 [Epub ahead of print].Search in Google Scholar PubMed PubMed Central

41. Kherd, AA, Helmi, N, Balamash, KS, Kumosani, TA, AL-Ghamdi, SA, Qari, M, et al.. Changes in erythrocyte ATPase activity under different pathological conditions. Afr Health Sci 2017;17:1204–10. https://doi.org/10.4314/ahs.v17i4.31.Search in Google Scholar PubMed PubMed Central

42. Maurya, PK, Prakash, S. Decreased activity of Ca(++)-ATPase and Na(+)/K(+)-ATPase during aging in humans. Appl Biochem Biotechnol 2013;170:131–7. https://doi.org/10.1007/s12010-013-0172-8.Search in Google Scholar PubMed

43. de, LAGR, Ordieres, MGL. Brain Na+, K+-ATPase activity in aging and disease. Int J Biomed Sci 2014;10:85–102. https://doi.org/10.59566/ijbs.2014.10085.Search in Google Scholar

44. Gutierrez-Mariscal, FM, Arenas-de Larriva, AP, Limia-Perez, L, Romero-Cabrera, JL, Yubero-Serrano, EM, López-Miranda, J. Coenzyme Q10 supplementation for the reduction of oxidative stress: clinical implications in the treatment of chronic diseases. Int J Mol Sci 2020;21:7870. https://doi.org/10.3390/ijms21217870.Search in Google Scholar PubMed PubMed Central

45. Zozina, VI, Covantev, S, Goroshko, OA, Krasnykh, LM, Kukes, VG. Coenzyme Q10 in cardiovascular and metabolic diseases: current state of the problem. Curr Cardiol Rev 2018;14:164–74. https://doi.org/10.2174/1573403x14666180416115428.Search in Google Scholar PubMed PubMed Central

46. Liu, J, Yan, Y, Nie, Y, Shapiro, J. Na/K-ATPase signaling and salt sensitivity: the role of oxidative stress. Antioxidants 2017;6:18. https://doi.org/10.3390/antiox6010018.Search in Google Scholar PubMed PubMed Central

47. Díaz-Casado, ME, Quiles, JL, Barriocanal-Casado, E, González-García, P, Battino, M, López, LC, et al.. The paradox of coenzyme Q10 in aging. Nutrients 2019;11:2221. https://doi.org/10.3390/nu11092221.Search in Google Scholar PubMed PubMed Central

48. Freye, ECK, Strobel, HP. Coenzyme Q10 supplements which increase ATP synthesis within mitochondria and protect against toxic sodium azide. Act Sci Nutr Health 2018;2:02–09.Search in Google Scholar

49. Kandhare, AD, Ghosh, P, Ghule, AE, Bodhankar, SL. Elucidation of molecular mechanism involved in neuroprotective effect of Coenzyme Q10 in alcohol-induced neuropathic pain. Fundam Clin Pharmacol 2013;27:603–22. https://doi.org/10.1111/fcp.12003.Search in Google Scholar PubMed

50. Fliegel, L. Sodium-hydrogen exchangers, structure and function in human health and disease. In: Kretsinger, RH, Uversky, VN, Permyakov, EA, editors. Encyclopedia of metalloproteins. New York, NY: Springer New York; 2013:2125–9 pp.10.1007/978-1-4614-1533-6_249Search in Google Scholar

51. Kant, R, Hu, Z, Malhotra, JK, Krogh-Madsen, T, Christini, DJ, Heerdt, PM, et al.. NHE isoform switching and KChIP2 upregulation in aging porcine atria. PLoS One 2013;8:e82951. https://doi.org/10.1371/journal.pone.0082951.Search in Google Scholar PubMed PubMed Central

52. Kumar, P, Chand, S, Maurya, PK. Quercetin-modulated erythrocyte membrane sodium-hydrogen exchanger during human aging: correlation with ATPase’s. Arch Physiol Biochem 2016;122:141–7. https://doi.org/10.3109/13813455.2016.1150299.Search in Google Scholar PubMed

53. Roberts, EL, Sick, TJ. Aging impairs regulation of intracellular pH in rat hippocampal slices. Brain Res 1996;735:339–42. https://doi.org/10.1016/0006-8993(96)00925-0.Search in Google Scholar PubMed

54. Heaton, RA, Heales, S, Rahman, K, Sexton, DW, Hargreaves, I. The effect of cellular coenzyme Q10 deficiency on lysosomal acidification. J Clin Med 2020;9:1923. https://doi.org/10.3390/jcm9061923.Search in Google Scholar PubMed PubMed Central

55. Ayala, A, Muñoz, MF, Argüelles, S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longevity 2014;2014:360438. https://doi.org/10.1155/2014/360438.Search in Google Scholar PubMed PubMed Central

56. Yavuzer, H, Yavuzer, S, Cengiz, M, Erman, H, Doventas, A, Balci, H, et al.. Biomarkers of lipid peroxidation related to hypertension in aging. Hypertens Res 2016;39:342–8. https://doi.org/10.1038/hr.2015.156.Search in Google Scholar PubMed

57. Iuchi, K, Takai, T, Hisatomi, H. Cell death via lipid peroxidation and protein aggregation diseases. Biology 2021;10:399. https://doi.org/10.3390/biology10050399.Search in Google Scholar PubMed PubMed Central

58. He, L, He, T, Farrar, S, Ji, L, Liu, T, Ma, X. Antioxidants maintain cellular redox homeostasis by elimination of reactive oxygen species. Cell Physiol Biochem 2017;44:532–53. https://doi.org/10.1159/000485089.Search in Google Scholar PubMed

59. Orbach, A, Zelig, O, Yedgar, S, Barshtein, G. Biophysical and biochemical markers of red blood cell fragility. Transfus Med Hemother 2017;44:183–7. https://doi.org/10.1159/000452106.Search in Google Scholar PubMed PubMed Central

60. Araki, K, Rifkind, JM. Age dependent changes in osmotic hemolysis of human erythrocytes. J Gerontol 1980;35:499–505. https://doi.org/10.1093/geronj/35.4.499.Search in Google Scholar PubMed

61. Ohls, RK. Developmental erythropoiesis. In: Fetal and neonatal physiology. Amsterdam, Netherlands: Elsevier; 2017, vol 2:1112–34.e4 pp.10.1016/B978-0-323-35214-7.00116-5Search in Google Scholar

62. Dariyerli, N, Toplan, S, Akyolcu, MC, Hatemi, H, Yigit, G. Erythrocyte osmotic fragility and oxidative stress in experimental hypothyroidism. Endocrine 2004;25:1–5. https://doi.org/10.1385/endo:25:1:01.10.1385/ENDO:25:1:01Search in Google Scholar PubMed

Received: 2024-06-02
Accepted: 2024-06-20
Published Online: 2024-07-05
Published in Print: 2025-03-26

© 2024 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/znc-2024-0129
Keywords for this article
aging; Coenzyme Q10; ionic balance; membrane transporters; oxidative stress

Articles in the same Issue

  1. Frontmatter
  2. Review Articles
  3. Advancing psoriasis drug delivery through topical liposomes
  4. Hepatoprotective activity of medicinal plants, their phytochemistry, and safety concerns: a systematic review
  5. Research Articles
  6. Phytochemical profile and antioxidant capacity of the endemic species Bellevalia sasonii Fidan
  7. In silico molecular modeling and in vitro biological screening of novel benzimidazole-based piperazine derivatives as potential acetylcholinesterase and butyrylcholinesterase inhibitors
  8. Coenzyme Q10 supplementation affects cellular ionic balance: relevance to aging
  9. Revolutionizing the probiotic functionality, biochemical activity, antibiotic resistance and specialty genes of Pediococcus acidilactici BCB1H via in-vitro and in-silico approaches
  10. Synthesis of modified Schiff base appended 1,2,4-triazole hybrids scaffolds: elucidating the in vitro and in silico α-amylase and α-glucosidase inhibitors potential
  11. Redefining a new frontier in alkaptonuria therapy with AI-driven drug candidate design via in-silico innovation
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