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Novel sialidase from non-pathogenic bacterium Oerskovia paurometabola strain O129

  • Rumyana T. Eneva ORCID logo EMAIL logo , Stephan A. Engibarov , Yana G. Gocheva ORCID logo , Simona L. Mitova and Penka M. Petrova
Published/Copyright: November 10, 2022
Zeitschrift für Naturforschung C
From the journal Zeitschrift für Naturforschung C Volume 78 Issue 1-2

Abstract

Bacterial sialidases are enzymes that are involved in a number of vital processes in microorganisms and in their interaction with the host or the environment. Their wide application for scientific and applied purposes requires the search for highly effective and non-pathogenic producers. Here, we report the first description of sialidase from Oerskovia paurometabola. The extracellular enzyme preparation was partially purified. The presence of sialidase was confirmed in native PAGE treated with the fluorogenic substrate 4MU-Neu5Ac. Maximum enzyme activity was registered at 37 °C and in the pH range of 4.0–5.5. The influence of metal ions and EDTA was examined. It was demonstrated that EDTA, Mn2+ and Ba2+ ions inhibit the sialidase activity to different extent, while Cd2+, Fe2+ and Fe3+ have stimulating effect on it. These features are studied for the first time concerning sialidase of Oerskovia representative. Cell bound sialidase and sialate aldolase were also established.

Keywords: Oerskovia ; pH optimum; sialate aldolase; sialidase; temperature optimum
Corresponding author: Rumyana T. Eneva, The Stephan Angeloff Institute of Microbiology, 1113 Sofia, Bulgaria, E-mail:

  1. Author contribution: All authors contributed to the study conception and design. All authors read and approved the final manuscript.

  2. Research funding: No funding was received for conducting this study.

  3. Conflict of interest statement: The authors declare that they have no conflict of interests.

  4. Compliance with ethical standards: This article does not contain any studies involving animal or human participants performed by any of the authors.

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/znc-2022-0051).

Received: 2022-03-10
Revised: 2022-06-14
Accepted: 2022-10-20
Published Online: 2022-11-10
Published in Print: 2023-01-27

© 2022 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/znc-2022-0051
Keywords for this article
Oerskovia; pH optimum; sialate aldolase; sialidase; temperature optimum

Articles in the same Issue

  1. Frontmatter
  2. Review Articles
  3. Covid-19 vaccines and neurological complications: a systematic review
  4. Santalum Genus: phytochemical constituents, biological activities and health promoting-effects
  5. Sakuranetin and its therapeutic potentials – a comprehensive review
  6. Novel sialidase from non-pathogenic bacterium Oerskovia paurometabola strain O129
  7. Do Colletotrichum gloeosporioides and Rhizopus stolonifer induce alkaloidal and antifungal responses in Annona muricata seedlings?
  8. Sesquiterpenoids from Tithonia diversifolia (Hemsl.) A. Gray induce apoptosis and inhibit the cell cycle progression of acute myeloid leukemia cells
  9. Isolation and purification of 12 flavonoid glycosides from Ginkgo biloba extract using sephadex LH-20 and preparative high-performance liquid chromatography
  10. Insecticidal activities of the essential oil of Rhynchanthus beesianus rhizomes and its constituents against two species of grain storage insects
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