Invertase adsorption with polymers functionalized by aspartic acid
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Kadir Erol
and
Şenol Yavuz
Abstract
Today, the separation and purification processes are highly preferred over the affinity interactions in the scientific world. Among the materials used for this purpose, magnetic particles and cryogels are very popular. Both polymeric structures have their advantages and disadvantages. In this study, poly(2-Hydroxyethyl methacrylate-N-methacryloyl-L-aspartic acid), poly(HEMA-MAsp), magnetic microparticles, and cryogels were synthesized, and adsorption performances of both polymeric structures were investigated by using invertase from aqueous systems. Invertase (β-fructofuranoside fructohydrolase, EC 3.2.1.26) is a commercially important enzyme used in the food industry to obtain the product called invert sugar, which consists of a mixture of equivalent amounts of glucose and fructose. Therefore, it was preferred as a model enzyme in adsorption studies of polymeric structures. According to the results, 104.1 mg g−1 and 135.5 mg g−1 of adsorption capacity values were obtained for cryogel and magnetic microparticle forms, respectively. Increasing temperature slightly reduced the adsorption capacity of both polymeric structures. In the adsorption/desorption cycle studies performed five times with poly(HEMA-MAsp) polymers, both forms were found to have high reusable properties. It was determined that the activity of invertase immobilized on polymeric structures was preserved at a rate of 83.6% for the particle form and 89.2% for the cryogel form.
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Author contribution: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
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Research funding: This study was supported by the Hitit University Scientific Research Projects Coordination Unit (grant no. ODMYO19001.18.001).
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Conflict of interest statement: The authors declare no conflicts of interest regarding this article.
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© 2022 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Material Properties
- Advancement in hemp fibre polymer composites: a comprehensive review
- Effects and mechanism of filler content on thermal conductivity of composites: a case study on plasticized polyvinyl chloride/graphite composites
- Effects of Eucommia ulmoides gum content and processing conditions on damping properties of E. ulmoides gum/nitrile-butadiene rubber nanocomposites
- Preparation and Assembly
- Preparation of flame retardant glass fiber via emulsion impregnation and application in polyamide 6
- Invertase adsorption with polymers functionalized by aspartic acid
- Engineering and Processing
- External field alignment of nickel-coated carbon fiber/PDMS composite for biological monitoring with high sensitivity
- Development of a cavity pressure control for injection moulding by adjusting the cross-section in the hot runner
- Process optimization for extraction of avian eggshell membrane derived collagen for tissue engineering applications
- Joint formation mechanism of different laser transmission welding paths
Articles in the same Issue
- Frontmatter
- Material Properties
- Advancement in hemp fibre polymer composites: a comprehensive review
- Effects and mechanism of filler content on thermal conductivity of composites: a case study on plasticized polyvinyl chloride/graphite composites
- Effects of Eucommia ulmoides gum content and processing conditions on damping properties of E. ulmoides gum/nitrile-butadiene rubber nanocomposites
- Preparation and Assembly
- Preparation of flame retardant glass fiber via emulsion impregnation and application in polyamide 6
- Invertase adsorption with polymers functionalized by aspartic acid
- Engineering and Processing
- External field alignment of nickel-coated carbon fiber/PDMS composite for biological monitoring with high sensitivity
- Development of a cavity pressure control for injection moulding by adjusting the cross-section in the hot runner
- Process optimization for extraction of avian eggshell membrane derived collagen for tissue engineering applications
- Joint formation mechanism of different laser transmission welding paths
