Process optimization for extraction of avian eggshell membrane derived collagen for tissue engineering applications

Aakriti Aggarwal; Mahesh Kumar Sah

doi:10.1515/polyeng-2021-0315

Article

Process optimization for extraction of avian eggshell membrane derived collagen for tissue engineering applications

Aakriti Aggarwal and Mahesh Kumar Sah

Published/Copyright: May 9, 2022

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From the journal Journal of Polymer Engineering Volume 42 Issue 7

Abstract

The avian eggshell membranes’ composition depicts close resemblance with the extracellular matrix of the cells, and therefore being widely employed as potential biomaterials for tissue engineering applications. However, the optimization of process conditions for collagen extraction, the main constituent of eggshell membranes is still challenging. In the present study, extraction of collagen was performed by an enzymatic method optimized through the one-factor-at-a-time (OFAT) technique for three parameters viz. pepsin concentration, treatment time and pH. The process optimization resulted in the maximum yield of 56% collagen with 350 U/mg pepsin concentration at pH 3 treated for 9 days, not reported yet. The collagen extraction was confirmed by OD at 232 nm; and its viscoelasticity behaviour at pH 5. The physico–chemical characterization of extracted collagen with FESEM, ATR-FTIR, surface roughness analysis and contact angle measurement revealed the morphological and topological alteration during the collagen extraction. The process optimization and characterization of eggshell membrane derived collagen can aid in the significant biomaterials development for tissue regeneration.

Keywords: biopolymer; collagen; eggshell membrane; OFAT; protein yield

Corresponding author: Mahesh Kumar Sah, Department of Biotechnology, Dr. B. R. Ambedkar National Institute of Technology, Jalandhar, Punjab 144011, India, E-mail: sahmk@nitj.ac.in

Acknowledgments

We are also thankful to Dr. Giri Babu (Dept. of Chemical Engineering) for carrying out viscosity studies, Dr. Vickramjeet Singh (Dept. of Chemistry) for contact angle measurements, and Dr. A. Chatterjee (Dept. of Textile Technology) for ATR-FTIR measurements.

Author contribution: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: We acknowledge the support from MHRD (Govt. of India) to Aakriti Aggarwal for completion of this research work.
Conflict of interest statement: The authors declare that they have no conflicts of interest regarding this article.

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Received: 2021-11-12

Revised: 2022-02-26

Accepted: 2022-03-23

Published Online: 2022-05-09

Published in Print: 2022-08-26

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Articles in the same Issue

https://doi.org/10.1515/polyeng-2021-0315

Keywords for this article

biopolymer; collagen; eggshell membrane; OFAT; protein yield